Cargando…

Maturity onset diabetes of the young and fibrin-related thrombosis risk

BACKGROUND: Fibrin network characteristics determine predisposition to cardiovascular disease (CVD). Individuals with type 1 (T1DM) and type 2 diabetes mellitus (T2DM) have higher risk of CVD and display deranged fibrin network structure. Those with maturity onset diabetes of the young (MODY) may al...

Descripción completa

Detalles Bibliográficos
Autores principales: Sagar, RC, Phoenix, F, Thanabalasingham, G, Naseem, K, Ajjan, RA, Owen, KR
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919224/
https://www.ncbi.nlm.nih.gov/pubmed/33334146
http://dx.doi.org/10.1177/1479164120963048
_version_ 1783658094113325056
author Sagar, RC
Phoenix, F
Thanabalasingham, G
Naseem, K
Ajjan, RA
Owen, KR
author_facet Sagar, RC
Phoenix, F
Thanabalasingham, G
Naseem, K
Ajjan, RA
Owen, KR
author_sort Sagar, RC
collection PubMed
description BACKGROUND: Fibrin network characteristics determine predisposition to cardiovascular disease (CVD). Individuals with type 1 (T1DM) and type 2 diabetes mellitus (T2DM) have higher risk of CVD and display deranged fibrin network structure. Those with maturity onset diabetes of the young (MODY) may also be at increased risk but their fibrin clot properties have not been studied. METHODS: Plasma clots properties from 13 individuals with HNF1A-MODY, 12 matched-individuals with T2DM and 12 with T1DM were studied using a validated turbidimetric assay and confocal microscopy. Plasma levels of fibrinogen, plasminogen activator inhibitor-1, complement C3 and C-reactive protein were also measured. RESULTS: MODY clot maximum absorbance was 0.37 ± 0.03 AU, similar to T1DM (0.32 ± 0.03 AU; p = 0.26), but lower than T2DM (0.49 ± 0.03 AU; p = 0.02), with confocal microscopy confirming structural differences. Clot lysis time in MODY was similar to T1DM (456 ± 50 and 402 ± 20 s, respectively; p = 0.09) but shorter than T2DM (588 ± 58 s; p = 0.006). Comparing inflammatory/thrombotic proteins in HNF1A-MODY and T2DM, C3 levels were lower in MODY than T2DM (0.58 ± 0.09 and 0.80 ± 0.1 mg/ml, respectively; p < 0.01). CONCLUSIONS: HNF1A-MODY fibrin network alterations are at least as pronounced as in T1DM but less thrombotic than T2DM clots. Differences in fibrin clot characteristics comparing HNF1A-MODY and T2DM may, in part, relate to lower C3 levels.
format Online
Article
Text
id pubmed-7919224
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-79192242021-03-02 Maturity onset diabetes of the young and fibrin-related thrombosis risk Sagar, RC Phoenix, F Thanabalasingham, G Naseem, K Ajjan, RA Owen, KR Diab Vasc Dis Res Brief Report BACKGROUND: Fibrin network characteristics determine predisposition to cardiovascular disease (CVD). Individuals with type 1 (T1DM) and type 2 diabetes mellitus (T2DM) have higher risk of CVD and display deranged fibrin network structure. Those with maturity onset diabetes of the young (MODY) may also be at increased risk but their fibrin clot properties have not been studied. METHODS: Plasma clots properties from 13 individuals with HNF1A-MODY, 12 matched-individuals with T2DM and 12 with T1DM were studied using a validated turbidimetric assay and confocal microscopy. Plasma levels of fibrinogen, plasminogen activator inhibitor-1, complement C3 and C-reactive protein were also measured. RESULTS: MODY clot maximum absorbance was 0.37 ± 0.03 AU, similar to T1DM (0.32 ± 0.03 AU; p = 0.26), but lower than T2DM (0.49 ± 0.03 AU; p = 0.02), with confocal microscopy confirming structural differences. Clot lysis time in MODY was similar to T1DM (456 ± 50 and 402 ± 20 s, respectively; p = 0.09) but shorter than T2DM (588 ± 58 s; p = 0.006). Comparing inflammatory/thrombotic proteins in HNF1A-MODY and T2DM, C3 levels were lower in MODY than T2DM (0.58 ± 0.09 and 0.80 ± 0.1 mg/ml, respectively; p < 0.01). CONCLUSIONS: HNF1A-MODY fibrin network alterations are at least as pronounced as in T1DM but less thrombotic than T2DM clots. Differences in fibrin clot characteristics comparing HNF1A-MODY and T2DM may, in part, relate to lower C3 levels. SAGE Publications 2020-12-18 /pmc/articles/PMC7919224/ /pubmed/33334146 http://dx.doi.org/10.1177/1479164120963048 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Brief Report
Sagar, RC
Phoenix, F
Thanabalasingham, G
Naseem, K
Ajjan, RA
Owen, KR
Maturity onset diabetes of the young and fibrin-related thrombosis risk
title Maturity onset diabetes of the young and fibrin-related thrombosis risk
title_full Maturity onset diabetes of the young and fibrin-related thrombosis risk
title_fullStr Maturity onset diabetes of the young and fibrin-related thrombosis risk
title_full_unstemmed Maturity onset diabetes of the young and fibrin-related thrombosis risk
title_short Maturity onset diabetes of the young and fibrin-related thrombosis risk
title_sort maturity onset diabetes of the young and fibrin-related thrombosis risk
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919224/
https://www.ncbi.nlm.nih.gov/pubmed/33334146
http://dx.doi.org/10.1177/1479164120963048
work_keys_str_mv AT sagarrc maturityonsetdiabetesoftheyoungandfibrinrelatedthrombosisrisk
AT phoenixf maturityonsetdiabetesoftheyoungandfibrinrelatedthrombosisrisk
AT thanabalasinghamg maturityonsetdiabetesoftheyoungandfibrinrelatedthrombosisrisk
AT naseemk maturityonsetdiabetesoftheyoungandfibrinrelatedthrombosisrisk
AT ajjanra maturityonsetdiabetesoftheyoungandfibrinrelatedthrombosisrisk
AT owenkr maturityonsetdiabetesoftheyoungandfibrinrelatedthrombosisrisk