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Prevalence of Strongyloidiasis in a Cohort of Migrants in Italy and Accuracy of a Novel ELISA Assay for S. stercoralis Infection, a Cross-Sectional Study

Strongyloides stercoralis infection is a life-threatening neglected tropical disease. Diagnostic issues have caused an underestimation of its global burden. The choice of appropriate diagnostic tests for the screening of populations at risk of the infection, such as migrants from endemic countries,...

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Autores principales: Buonfrate, Dora, Marrone, Rosalia, Silva, Ronaldo, Mirisola, Concetta, Ragusa, Andrea, Mistretta, Manuela, Perandin, Francesca, Bisoffi, Zeno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919371/
https://www.ncbi.nlm.nih.gov/pubmed/33671980
http://dx.doi.org/10.3390/microorganisms9020401
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author Buonfrate, Dora
Marrone, Rosalia
Silva, Ronaldo
Mirisola, Concetta
Ragusa, Andrea
Mistretta, Manuela
Perandin, Francesca
Bisoffi, Zeno
author_facet Buonfrate, Dora
Marrone, Rosalia
Silva, Ronaldo
Mirisola, Concetta
Ragusa, Andrea
Mistretta, Manuela
Perandin, Francesca
Bisoffi, Zeno
author_sort Buonfrate, Dora
collection PubMed
description Strongyloides stercoralis infection is a life-threatening neglected tropical disease. Diagnostic issues have caused an underestimation of its global burden. The choice of appropriate diagnostic tests for the screening of populations at risk of the infection, such as migrants from endemic countries, is of paramount importance. From November 2017 to July 2018, all migrants presenting to the National Institute for Health Migration and Poverty (INMP) in Rome, Italy were offered screening tests for S. stercoralis infection. The study objective was to estimate the prevalence of strongyloidiasis in the study population and the accuracy of a novel ELISA assay. The following tests were carried out at the IRCCS Sacro Cuore Don Calabria hospital in Negrar, Verona: stool microscopy, real-time PCR for S. stercoralis, in-house immunofluorescence test (IFAT), a commercial ELISA assay (Bordier ELISA), and a novel ELISA assay (Euroimmun ELISA). A latent class analysis (LCA) model set up with test results, clinical variables, and eosinophilia indicated a prevalence around 7.5%, in line with previous findings. The sensitivity and the specificity of Euroimmun ELISA were 90.6% (95% CI 80.5–100) and 87.7% (95CI 84.5–91.0); these results indicate that the novel ELISA assay would be suitable for screening of migrants from endemic countries.
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spelling pubmed-79193712021-03-02 Prevalence of Strongyloidiasis in a Cohort of Migrants in Italy and Accuracy of a Novel ELISA Assay for S. stercoralis Infection, a Cross-Sectional Study Buonfrate, Dora Marrone, Rosalia Silva, Ronaldo Mirisola, Concetta Ragusa, Andrea Mistretta, Manuela Perandin, Francesca Bisoffi, Zeno Microorganisms Communication Strongyloides stercoralis infection is a life-threatening neglected tropical disease. Diagnostic issues have caused an underestimation of its global burden. The choice of appropriate diagnostic tests for the screening of populations at risk of the infection, such as migrants from endemic countries, is of paramount importance. From November 2017 to July 2018, all migrants presenting to the National Institute for Health Migration and Poverty (INMP) in Rome, Italy were offered screening tests for S. stercoralis infection. The study objective was to estimate the prevalence of strongyloidiasis in the study population and the accuracy of a novel ELISA assay. The following tests were carried out at the IRCCS Sacro Cuore Don Calabria hospital in Negrar, Verona: stool microscopy, real-time PCR for S. stercoralis, in-house immunofluorescence test (IFAT), a commercial ELISA assay (Bordier ELISA), and a novel ELISA assay (Euroimmun ELISA). A latent class analysis (LCA) model set up with test results, clinical variables, and eosinophilia indicated a prevalence around 7.5%, in line with previous findings. The sensitivity and the specificity of Euroimmun ELISA were 90.6% (95% CI 80.5–100) and 87.7% (95CI 84.5–91.0); these results indicate that the novel ELISA assay would be suitable for screening of migrants from endemic countries. MDPI 2021-02-15 /pmc/articles/PMC7919371/ /pubmed/33671980 http://dx.doi.org/10.3390/microorganisms9020401 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Buonfrate, Dora
Marrone, Rosalia
Silva, Ronaldo
Mirisola, Concetta
Ragusa, Andrea
Mistretta, Manuela
Perandin, Francesca
Bisoffi, Zeno
Prevalence of Strongyloidiasis in a Cohort of Migrants in Italy and Accuracy of a Novel ELISA Assay for S. stercoralis Infection, a Cross-Sectional Study
title Prevalence of Strongyloidiasis in a Cohort of Migrants in Italy and Accuracy of a Novel ELISA Assay for S. stercoralis Infection, a Cross-Sectional Study
title_full Prevalence of Strongyloidiasis in a Cohort of Migrants in Italy and Accuracy of a Novel ELISA Assay for S. stercoralis Infection, a Cross-Sectional Study
title_fullStr Prevalence of Strongyloidiasis in a Cohort of Migrants in Italy and Accuracy of a Novel ELISA Assay for S. stercoralis Infection, a Cross-Sectional Study
title_full_unstemmed Prevalence of Strongyloidiasis in a Cohort of Migrants in Italy and Accuracy of a Novel ELISA Assay for S. stercoralis Infection, a Cross-Sectional Study
title_short Prevalence of Strongyloidiasis in a Cohort of Migrants in Italy and Accuracy of a Novel ELISA Assay for S. stercoralis Infection, a Cross-Sectional Study
title_sort prevalence of strongyloidiasis in a cohort of migrants in italy and accuracy of a novel elisa assay for s. stercoralis infection, a cross-sectional study
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919371/
https://www.ncbi.nlm.nih.gov/pubmed/33671980
http://dx.doi.org/10.3390/microorganisms9020401
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