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Comparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers

SIMPLE SUMMARY: Equine atypical myopathy is a muscular disease caused by a plant intoxication, which seems to affect only certain horses sharing a pasture, and the role of the intestinal bacteria (microbiota) in this selective impairment is still poorly understood. The aim of this study was to descr...

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Autores principales: Wimmer-Scherr, Christina, Taminiau, Bernard, Renaud, Benoît, van Loon, Gunther, Palmers, Katrien, Votion, Dominique, Amory, Hélène, Daube, Georges, Cesarini, Carla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919468/
https://www.ncbi.nlm.nih.gov/pubmed/33672034
http://dx.doi.org/10.3390/ani11020506
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author Wimmer-Scherr, Christina
Taminiau, Bernard
Renaud, Benoît
van Loon, Gunther
Palmers, Katrien
Votion, Dominique
Amory, Hélène
Daube, Georges
Cesarini, Carla
author_facet Wimmer-Scherr, Christina
Taminiau, Bernard
Renaud, Benoît
van Loon, Gunther
Palmers, Katrien
Votion, Dominique
Amory, Hélène
Daube, Georges
Cesarini, Carla
author_sort Wimmer-Scherr, Christina
collection PubMed
description SIMPLE SUMMARY: Equine atypical myopathy is a muscular disease caused by a plant intoxication, which seems to affect only certain horses sharing a pasture, and the role of the intestinal bacteria (microbiota) in this selective impairment is still poorly understood. The aim of this study was to describe and compare fecal microbiota of horses suffering from atypical myopathy and healthy co-grazers. We concluded that fecal microbiota of horses suffering from atypical myopathy is different from their co-grazers and changes are more severe in horses that do not survive the disease. Understanding those changes may help in developing therapeutic and/or preventive strategies for horses at risk for atypical myopathy. ABSTRACT: Equine atypical myopathy (AM) is caused by hypoglycin A (HGA) and methylenecyclopropylglycine (MCPG) intoxication resulting from the ingestion of seeds or seedlings of some Acer tree species. Interestingly, not all horses pasturing in the same toxic environment develop signs of the disease. In other species, it has been shown that the intestinal microbiota has an impact on digestion, metabolism, immune stimulation and protection from disease. The objective of this study was to characterize and compare fecal microbiota of horses suffering from AM and healthy co-grazers. Furthermore, potential differences in fecal microbiota regarding the outcome of diseased animals were assessed. This prospective observational study included 59 horses with AM (29 survivors and 30 non-survivors) referred to three Belgian equine hospitals and 26 clinically healthy co-grazers simultaneously sharing contaminated pastures during spring and autumn outbreak periods. Fresh fecal samples (rectal or within 30 min of defecation) were obtained from all horses and bacterial taxonomy profiling obtained by 16S amplicon sequencing was used to identify differentially distributed bacterial taxa between AM-affected horses and healthy co-grazers. Fecal microbial diversity and evenness were significantly (p < 0.001) higher in AM-affected horses as compared with their non-affected co-grazers. The relative abundance of families Ruminococcaceae, Christensenellaceae and Akkermansiaceae were higher (p ≤ 0.001) whereas those of the Lachnospiraceae (p = 0.0053), Bacteroidales (p < 0.0001) and Clostridiales (p = 0.0402) were lower in horses with AM, especially in those with a poor prognosis. While significant shifts were observed, it is still unclear whether they result from the disease or might be involved in the onset of disease pathogenesis.
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spelling pubmed-79194682021-03-02 Comparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers Wimmer-Scherr, Christina Taminiau, Bernard Renaud, Benoît van Loon, Gunther Palmers, Katrien Votion, Dominique Amory, Hélène Daube, Georges Cesarini, Carla Animals (Basel) Article SIMPLE SUMMARY: Equine atypical myopathy is a muscular disease caused by a plant intoxication, which seems to affect only certain horses sharing a pasture, and the role of the intestinal bacteria (microbiota) in this selective impairment is still poorly understood. The aim of this study was to describe and compare fecal microbiota of horses suffering from atypical myopathy and healthy co-grazers. We concluded that fecal microbiota of horses suffering from atypical myopathy is different from their co-grazers and changes are more severe in horses that do not survive the disease. Understanding those changes may help in developing therapeutic and/or preventive strategies for horses at risk for atypical myopathy. ABSTRACT: Equine atypical myopathy (AM) is caused by hypoglycin A (HGA) and methylenecyclopropylglycine (MCPG) intoxication resulting from the ingestion of seeds or seedlings of some Acer tree species. Interestingly, not all horses pasturing in the same toxic environment develop signs of the disease. In other species, it has been shown that the intestinal microbiota has an impact on digestion, metabolism, immune stimulation and protection from disease. The objective of this study was to characterize and compare fecal microbiota of horses suffering from AM and healthy co-grazers. Furthermore, potential differences in fecal microbiota regarding the outcome of diseased animals were assessed. This prospective observational study included 59 horses with AM (29 survivors and 30 non-survivors) referred to three Belgian equine hospitals and 26 clinically healthy co-grazers simultaneously sharing contaminated pastures during spring and autumn outbreak periods. Fresh fecal samples (rectal or within 30 min of defecation) were obtained from all horses and bacterial taxonomy profiling obtained by 16S amplicon sequencing was used to identify differentially distributed bacterial taxa between AM-affected horses and healthy co-grazers. Fecal microbial diversity and evenness were significantly (p < 0.001) higher in AM-affected horses as compared with their non-affected co-grazers. The relative abundance of families Ruminococcaceae, Christensenellaceae and Akkermansiaceae were higher (p ≤ 0.001) whereas those of the Lachnospiraceae (p = 0.0053), Bacteroidales (p < 0.0001) and Clostridiales (p = 0.0402) were lower in horses with AM, especially in those with a poor prognosis. While significant shifts were observed, it is still unclear whether they result from the disease or might be involved in the onset of disease pathogenesis. MDPI 2021-02-15 /pmc/articles/PMC7919468/ /pubmed/33672034 http://dx.doi.org/10.3390/ani11020506 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wimmer-Scherr, Christina
Taminiau, Bernard
Renaud, Benoît
van Loon, Gunther
Palmers, Katrien
Votion, Dominique
Amory, Hélène
Daube, Georges
Cesarini, Carla
Comparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers
title Comparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers
title_full Comparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers
title_fullStr Comparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers
title_full_unstemmed Comparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers
title_short Comparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers
title_sort comparison of fecal microbiota of horses suffering from atypical myopathy and healthy co-grazers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919468/
https://www.ncbi.nlm.nih.gov/pubmed/33672034
http://dx.doi.org/10.3390/ani11020506
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