Cargando…
The Acute Effects of Swimming Exercise on PGC-1α-FNDC5/Irisin-UCP1 Expression in Male C57BL/6J Mice
Irisin is a myokine primarily secreted by skeletal muscles and is known as an exercise-induced hormone. The purpose of this study was to determine whether the PGC-1α -FNDC5 /Irisin-UCP1 expression which is an irisin-related signaling pathway, is activated by an acute swimming exercise. Fourteen to s...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919784/ https://www.ncbi.nlm.nih.gov/pubmed/33669194 http://dx.doi.org/10.3390/metabo11020111 |
_version_ | 1783658180928077824 |
---|---|
author | Cho, Eunhee Jeong, Da Yeon Kim, Jae Geun Lee, Sewon |
author_facet | Cho, Eunhee Jeong, Da Yeon Kim, Jae Geun Lee, Sewon |
author_sort | Cho, Eunhee |
collection | PubMed |
description | Irisin is a myokine primarily secreted by skeletal muscles and is known as an exercise-induced hormone. The purpose of this study was to determine whether the PGC-1α -FNDC5 /Irisin-UCP1 expression which is an irisin-related signaling pathway, is activated by an acute swimming exercise. Fourteen to sixteen weeks old male C57BL/6J mice (n = 20) were divided into control (CON, n = 10) and swimming exercise groups (SEG, n = 10). The SEG mice performed 90 min of acute swimming exercise, while control (non-exercised) mice were exposed to shallow water (2 cm of depth) for 90 min. The mRNA and protein expression of PGC-1α, FNDC5 and browning markers including UCP1 were evaluated by quantitative real-time PCR and western blotting. Serum irisin concentration was measured by enzyme-linked immunosorbent assay. An acute swimming exercise did not lead to alterations in the mRNA and protein expression of PGC-1α in both soleus and gastrocnemius muscles, the mRNA and protein expression of UCP1 in brown adipose tissue, mRNA browning markers in visceral adipose tissue and circulating irisin when compared with the control group. On the other hand, an acute swimming exercise led to increases in the mRNA and protein expressions of FNDC5 in the soleus muscle, the protein expression of FNDC5 in the gastrocnemius muscles and the protein expression of UCP1 in subcutaneous adipose tissue. |
format | Online Article Text |
id | pubmed-7919784 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79197842021-03-02 The Acute Effects of Swimming Exercise on PGC-1α-FNDC5/Irisin-UCP1 Expression in Male C57BL/6J Mice Cho, Eunhee Jeong, Da Yeon Kim, Jae Geun Lee, Sewon Metabolites Article Irisin is a myokine primarily secreted by skeletal muscles and is known as an exercise-induced hormone. The purpose of this study was to determine whether the PGC-1α -FNDC5 /Irisin-UCP1 expression which is an irisin-related signaling pathway, is activated by an acute swimming exercise. Fourteen to sixteen weeks old male C57BL/6J mice (n = 20) were divided into control (CON, n = 10) and swimming exercise groups (SEG, n = 10). The SEG mice performed 90 min of acute swimming exercise, while control (non-exercised) mice were exposed to shallow water (2 cm of depth) for 90 min. The mRNA and protein expression of PGC-1α, FNDC5 and browning markers including UCP1 were evaluated by quantitative real-time PCR and western blotting. Serum irisin concentration was measured by enzyme-linked immunosorbent assay. An acute swimming exercise did not lead to alterations in the mRNA and protein expression of PGC-1α in both soleus and gastrocnemius muscles, the mRNA and protein expression of UCP1 in brown adipose tissue, mRNA browning markers in visceral adipose tissue and circulating irisin when compared with the control group. On the other hand, an acute swimming exercise led to increases in the mRNA and protein expressions of FNDC5 in the soleus muscle, the protein expression of FNDC5 in the gastrocnemius muscles and the protein expression of UCP1 in subcutaneous adipose tissue. MDPI 2021-02-16 /pmc/articles/PMC7919784/ /pubmed/33669194 http://dx.doi.org/10.3390/metabo11020111 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cho, Eunhee Jeong, Da Yeon Kim, Jae Geun Lee, Sewon The Acute Effects of Swimming Exercise on PGC-1α-FNDC5/Irisin-UCP1 Expression in Male C57BL/6J Mice |
title | The Acute Effects of Swimming Exercise on PGC-1α-FNDC5/Irisin-UCP1 Expression in Male C57BL/6J Mice |
title_full | The Acute Effects of Swimming Exercise on PGC-1α-FNDC5/Irisin-UCP1 Expression in Male C57BL/6J Mice |
title_fullStr | The Acute Effects of Swimming Exercise on PGC-1α-FNDC5/Irisin-UCP1 Expression in Male C57BL/6J Mice |
title_full_unstemmed | The Acute Effects of Swimming Exercise on PGC-1α-FNDC5/Irisin-UCP1 Expression in Male C57BL/6J Mice |
title_short | The Acute Effects of Swimming Exercise on PGC-1α-FNDC5/Irisin-UCP1 Expression in Male C57BL/6J Mice |
title_sort | acute effects of swimming exercise on pgc-1α-fndc5/irisin-ucp1 expression in male c57bl/6j mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919784/ https://www.ncbi.nlm.nih.gov/pubmed/33669194 http://dx.doi.org/10.3390/metabo11020111 |
work_keys_str_mv | AT choeunhee theacuteeffectsofswimmingexerciseonpgc1afndc5irisinucp1expressioninmalec57bl6jmice AT jeongdayeon theacuteeffectsofswimmingexerciseonpgc1afndc5irisinucp1expressioninmalec57bl6jmice AT kimjaegeun theacuteeffectsofswimmingexerciseonpgc1afndc5irisinucp1expressioninmalec57bl6jmice AT leesewon theacuteeffectsofswimmingexerciseonpgc1afndc5irisinucp1expressioninmalec57bl6jmice AT choeunhee acuteeffectsofswimmingexerciseonpgc1afndc5irisinucp1expressioninmalec57bl6jmice AT jeongdayeon acuteeffectsofswimmingexerciseonpgc1afndc5irisinucp1expressioninmalec57bl6jmice AT kimjaegeun acuteeffectsofswimmingexerciseonpgc1afndc5irisinucp1expressioninmalec57bl6jmice AT leesewon acuteeffectsofswimmingexerciseonpgc1afndc5irisinucp1expressioninmalec57bl6jmice |