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Rutin-Loaded Solid Lipid Nanoparticles: Characterization and In Vitro Evaluation
This study was aimed at preparing and characterizing solid lipid nanoparticles loading rutin (RT-SLNs) for the treatment of oxidative stress-induced diseases. Phospholipon 80H(®) as a solid lipid and Polysorbate 80 as surfactant were used for the SLNs preparation, using the solvent emulsification/di...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920302/ https://www.ncbi.nlm.nih.gov/pubmed/33669321 http://dx.doi.org/10.3390/molecules26041039 |
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author | De Gaetano, Federica Cristiano, Maria Chiara Venuti, Valentina Crupi, Vincenza Majolino, Domenico Paladini, Giuseppe Acri, Giuseppe Testagrossa, Barbara Irrera, Alessia Paolino, Donatella Tommasini, Silvana Ventura, Cinzia Anna Stancanelli, Rosanna |
author_facet | De Gaetano, Federica Cristiano, Maria Chiara Venuti, Valentina Crupi, Vincenza Majolino, Domenico Paladini, Giuseppe Acri, Giuseppe Testagrossa, Barbara Irrera, Alessia Paolino, Donatella Tommasini, Silvana Ventura, Cinzia Anna Stancanelli, Rosanna |
author_sort | De Gaetano, Federica |
collection | PubMed |
description | This study was aimed at preparing and characterizing solid lipid nanoparticles loading rutin (RT-SLNs) for the treatment of oxidative stress-induced diseases. Phospholipon 80H(®) as a solid lipid and Polysorbate 80 as surfactant were used for the SLNs preparation, using the solvent emulsification/diffusion method. We obtained spherical RT-SLNs with low sizes, ranging from 40 to 60 nm (hydrodynamic radius) for the SLNs prepared starting from 2% and 5% (w/w) theoretical amount. All prepared formulations showed negative zeta-potential values. RT was efficiently encapsulated within SLNs, obtaining high encapsulation efficiency and drug content percentages, particularly for SLNs prepared with a 5% theoretical amount of RT. In vitro release profiles and analysis of the obtained data applying different kinetic models revealed Fickian diffusion as the main mechanism of RT release from the SLNs. The morphology of RT-SLNs was characterized by scanning electron microscopy (SEM), whereas the interactions between RT and the lipid matrix were investigated by Raman spectroscopy, evidencing spectral modifications of characteristic bands of RT due to the establishment of new interactions. Finally, antioxidant activity assay on human glioblastoma astrocytoma (U373) culture cells showed a dose-dependent activity for RT-SLNs, particularly at the highest assayed dose (50 μM), whereas the free drug showed the lesser activity. |
format | Online Article Text |
id | pubmed-7920302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79203022021-03-02 Rutin-Loaded Solid Lipid Nanoparticles: Characterization and In Vitro Evaluation De Gaetano, Federica Cristiano, Maria Chiara Venuti, Valentina Crupi, Vincenza Majolino, Domenico Paladini, Giuseppe Acri, Giuseppe Testagrossa, Barbara Irrera, Alessia Paolino, Donatella Tommasini, Silvana Ventura, Cinzia Anna Stancanelli, Rosanna Molecules Article This study was aimed at preparing and characterizing solid lipid nanoparticles loading rutin (RT-SLNs) for the treatment of oxidative stress-induced diseases. Phospholipon 80H(®) as a solid lipid and Polysorbate 80 as surfactant were used for the SLNs preparation, using the solvent emulsification/diffusion method. We obtained spherical RT-SLNs with low sizes, ranging from 40 to 60 nm (hydrodynamic radius) for the SLNs prepared starting from 2% and 5% (w/w) theoretical amount. All prepared formulations showed negative zeta-potential values. RT was efficiently encapsulated within SLNs, obtaining high encapsulation efficiency and drug content percentages, particularly for SLNs prepared with a 5% theoretical amount of RT. In vitro release profiles and analysis of the obtained data applying different kinetic models revealed Fickian diffusion as the main mechanism of RT release from the SLNs. The morphology of RT-SLNs was characterized by scanning electron microscopy (SEM), whereas the interactions between RT and the lipid matrix were investigated by Raman spectroscopy, evidencing spectral modifications of characteristic bands of RT due to the establishment of new interactions. Finally, antioxidant activity assay on human glioblastoma astrocytoma (U373) culture cells showed a dose-dependent activity for RT-SLNs, particularly at the highest assayed dose (50 μM), whereas the free drug showed the lesser activity. MDPI 2021-02-16 /pmc/articles/PMC7920302/ /pubmed/33669321 http://dx.doi.org/10.3390/molecules26041039 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article De Gaetano, Federica Cristiano, Maria Chiara Venuti, Valentina Crupi, Vincenza Majolino, Domenico Paladini, Giuseppe Acri, Giuseppe Testagrossa, Barbara Irrera, Alessia Paolino, Donatella Tommasini, Silvana Ventura, Cinzia Anna Stancanelli, Rosanna Rutin-Loaded Solid Lipid Nanoparticles: Characterization and In Vitro Evaluation |
title | Rutin-Loaded Solid Lipid Nanoparticles: Characterization and In Vitro Evaluation |
title_full | Rutin-Loaded Solid Lipid Nanoparticles: Characterization and In Vitro Evaluation |
title_fullStr | Rutin-Loaded Solid Lipid Nanoparticles: Characterization and In Vitro Evaluation |
title_full_unstemmed | Rutin-Loaded Solid Lipid Nanoparticles: Characterization and In Vitro Evaluation |
title_short | Rutin-Loaded Solid Lipid Nanoparticles: Characterization and In Vitro Evaluation |
title_sort | rutin-loaded solid lipid nanoparticles: characterization and in vitro evaluation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920302/ https://www.ncbi.nlm.nih.gov/pubmed/33669321 http://dx.doi.org/10.3390/molecules26041039 |
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