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Sex Differences in Photoprotective Responses to 1,25-Dihydroxyvitamin D3 in Mice Are Modulated by the Estrogen Receptor-β

Susceptibility to photoimmune suppression and photocarcinogenesis is greater in male than in female humans and mice and is exacerbated in female estrogen receptor-beta knockout (ER-β−/−) mice. We previously reported that the active vitamin D hormone, 1,25-dihydroxyvitamin D3 (1,25(OH)2D), applied to...

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Autores principales: Tongkao-on, Wannit, Yang, Chen, McCarthy, Bianca Y., De Silva, Warusavithana G. Manori, Rybchyn, Mark S., Gordon-Thomson, Clare, Dixon, Katie M., Halliday, Gary M., Reeve, Vivienne E., Mason, Rebecca S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920427/
https://www.ncbi.nlm.nih.gov/pubmed/33669452
http://dx.doi.org/10.3390/ijms22041962
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author Tongkao-on, Wannit
Yang, Chen
McCarthy, Bianca Y.
De Silva, Warusavithana G. Manori
Rybchyn, Mark S.
Gordon-Thomson, Clare
Dixon, Katie M.
Halliday, Gary M.
Reeve, Vivienne E.
Mason, Rebecca S.
author_facet Tongkao-on, Wannit
Yang, Chen
McCarthy, Bianca Y.
De Silva, Warusavithana G. Manori
Rybchyn, Mark S.
Gordon-Thomson, Clare
Dixon, Katie M.
Halliday, Gary M.
Reeve, Vivienne E.
Mason, Rebecca S.
author_sort Tongkao-on, Wannit
collection PubMed
description Susceptibility to photoimmune suppression and photocarcinogenesis is greater in male than in female humans and mice and is exacerbated in female estrogen receptor-beta knockout (ER-β−/−) mice. We previously reported that the active vitamin D hormone, 1,25-dihydroxyvitamin D3 (1,25(OH)2D), applied topically protects against the ultraviolet radiation (UV) induction of cutaneous cyclobutane pyrimidine dimers (CPDs) and the suppression of contact hypersensitivity (CHS) in female mice. Here, we compare these responses in female versus male Skh:hr1 mice, in ER-β−/−/−− versus wild-type C57BL/6 mice, and in female ER-blockaded Skh:hr1 mice. The induction of CPDs was significantly greater in male than female Skh:hr1 mice and was more effectively reduced by 1,25(OH)2D in female Skh:hr1 and C57BL/6 mice than in male Skh:hr1 or ER-β−/− mice, respectively. This correlated with the reduced sunburn inflammation due to 1,25(OH)2D in female but not male Skh:hr1 mice. Furthermore, although 1,25(OH)2D alone dose-dependently suppressed basal CHS responses in male Skh:hr1 and ER-β−/− mice, UV-induced immunosuppression was universally observed. In female Skh:hr1 and C57BL/6 mice, the immunosuppression was decreased by 1,25(OH)2D dose-dependently, but not in male Skh:hr1, ER-β−/−, or ER-blockaded mice. These results reveal a sex bias in genetic, inflammatory, and immune photoprotection by 1,25(OH)2D favoring female mice that is dependent on the presence of ER-β.
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spelling pubmed-79204272021-03-02 Sex Differences in Photoprotective Responses to 1,25-Dihydroxyvitamin D3 in Mice Are Modulated by the Estrogen Receptor-β Tongkao-on, Wannit Yang, Chen McCarthy, Bianca Y. De Silva, Warusavithana G. Manori Rybchyn, Mark S. Gordon-Thomson, Clare Dixon, Katie M. Halliday, Gary M. Reeve, Vivienne E. Mason, Rebecca S. Int J Mol Sci Article Susceptibility to photoimmune suppression and photocarcinogenesis is greater in male than in female humans and mice and is exacerbated in female estrogen receptor-beta knockout (ER-β−/−) mice. We previously reported that the active vitamin D hormone, 1,25-dihydroxyvitamin D3 (1,25(OH)2D), applied topically protects against the ultraviolet radiation (UV) induction of cutaneous cyclobutane pyrimidine dimers (CPDs) and the suppression of contact hypersensitivity (CHS) in female mice. Here, we compare these responses in female versus male Skh:hr1 mice, in ER-β−/−/−− versus wild-type C57BL/6 mice, and in female ER-blockaded Skh:hr1 mice. The induction of CPDs was significantly greater in male than female Skh:hr1 mice and was more effectively reduced by 1,25(OH)2D in female Skh:hr1 and C57BL/6 mice than in male Skh:hr1 or ER-β−/− mice, respectively. This correlated with the reduced sunburn inflammation due to 1,25(OH)2D in female but not male Skh:hr1 mice. Furthermore, although 1,25(OH)2D alone dose-dependently suppressed basal CHS responses in male Skh:hr1 and ER-β−/− mice, UV-induced immunosuppression was universally observed. In female Skh:hr1 and C57BL/6 mice, the immunosuppression was decreased by 1,25(OH)2D dose-dependently, but not in male Skh:hr1, ER-β−/−, or ER-blockaded mice. These results reveal a sex bias in genetic, inflammatory, and immune photoprotection by 1,25(OH)2D favoring female mice that is dependent on the presence of ER-β. MDPI 2021-02-16 /pmc/articles/PMC7920427/ /pubmed/33669452 http://dx.doi.org/10.3390/ijms22041962 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tongkao-on, Wannit
Yang, Chen
McCarthy, Bianca Y.
De Silva, Warusavithana G. Manori
Rybchyn, Mark S.
Gordon-Thomson, Clare
Dixon, Katie M.
Halliday, Gary M.
Reeve, Vivienne E.
Mason, Rebecca S.
Sex Differences in Photoprotective Responses to 1,25-Dihydroxyvitamin D3 in Mice Are Modulated by the Estrogen Receptor-β
title Sex Differences in Photoprotective Responses to 1,25-Dihydroxyvitamin D3 in Mice Are Modulated by the Estrogen Receptor-β
title_full Sex Differences in Photoprotective Responses to 1,25-Dihydroxyvitamin D3 in Mice Are Modulated by the Estrogen Receptor-β
title_fullStr Sex Differences in Photoprotective Responses to 1,25-Dihydroxyvitamin D3 in Mice Are Modulated by the Estrogen Receptor-β
title_full_unstemmed Sex Differences in Photoprotective Responses to 1,25-Dihydroxyvitamin D3 in Mice Are Modulated by the Estrogen Receptor-β
title_short Sex Differences in Photoprotective Responses to 1,25-Dihydroxyvitamin D3 in Mice Are Modulated by the Estrogen Receptor-β
title_sort sex differences in photoprotective responses to 1,25-dihydroxyvitamin d3 in mice are modulated by the estrogen receptor-β
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920427/
https://www.ncbi.nlm.nih.gov/pubmed/33669452
http://dx.doi.org/10.3390/ijms22041962
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