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An innovative and user-friendly smartphone-assisted molecular diagnostic approach for rapid detection of canine vector-borne diseases
Present-day diagnostic tools and technologies for canine diseases and other vector-borne parasitic diseases hardly meet the requirements of an efficient and rapid diagnostic tool, which can be suitable for use at the point-of-care in resource-limited settings. Loop-mediated isothermal amplification...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920752/ https://www.ncbi.nlm.nih.gov/pubmed/33649963 http://dx.doi.org/10.1007/s00436-021-07077-z |
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author | Upadhyay, Archana Waleed, Raza Muhammad Wang, Jinhua Zhao, Jianguo Guan, Qingfeng Liao, Chenghong Han, Qian |
author_facet | Upadhyay, Archana Waleed, Raza Muhammad Wang, Jinhua Zhao, Jianguo Guan, Qingfeng Liao, Chenghong Han, Qian |
author_sort | Upadhyay, Archana |
collection | PubMed |
description | Present-day diagnostic tools and technologies for canine diseases and other vector-borne parasitic diseases hardly meet the requirements of an efficient and rapid diagnostic tool, which can be suitable for use at the point-of-care in resource-limited settings. Loop-mediated isothermal amplification (LAMP) technique has been always a method of choice in the development and validation of quick, precise, and sensitive diagnostic assays for pathogen detection and to reorganize point-of-care (POC) molecular diagnostics. In this study, we have demonstrated an efficient detection system for parasitic vector-borne pathogens like Ehrlichia canis and Hepatozoon canis by linking the LAMP assay to a smartphone via a simple, inexpensive, and a portable “LAMP box,” All the components of the LAMP box were connected to each other wirelessly. This LAMP box was made up of an isothermal heating pad mounted below an aluminum base which served as a platform for the reaction tubes and LAMP assay. The entire setup could be connected to a smartphone via an inbuilt Wi-Fi that allowed the user to establish the connection to control the LAMP box. A 5 V USB power source was used as a power supply. The sensitivity of the LAMP assay was estimated to be up to 10(−6) dilution limit using the amplified, purified, and quantified specific DNA templates. It can also serve as an efficient diagnostic platform for many other veterinary infectious or parasitic diseases of zoonotic origin majorly towards field-based diagnostics. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00436-021-07077-z. |
format | Online Article Text |
id | pubmed-7920752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-79207522021-03-02 An innovative and user-friendly smartphone-assisted molecular diagnostic approach for rapid detection of canine vector-borne diseases Upadhyay, Archana Waleed, Raza Muhammad Wang, Jinhua Zhao, Jianguo Guan, Qingfeng Liao, Chenghong Han, Qian Parasitol Res Protozoology - Original Paper Present-day diagnostic tools and technologies for canine diseases and other vector-borne parasitic diseases hardly meet the requirements of an efficient and rapid diagnostic tool, which can be suitable for use at the point-of-care in resource-limited settings. Loop-mediated isothermal amplification (LAMP) technique has been always a method of choice in the development and validation of quick, precise, and sensitive diagnostic assays for pathogen detection and to reorganize point-of-care (POC) molecular diagnostics. In this study, we have demonstrated an efficient detection system for parasitic vector-borne pathogens like Ehrlichia canis and Hepatozoon canis by linking the LAMP assay to a smartphone via a simple, inexpensive, and a portable “LAMP box,” All the components of the LAMP box were connected to each other wirelessly. This LAMP box was made up of an isothermal heating pad mounted below an aluminum base which served as a platform for the reaction tubes and LAMP assay. The entire setup could be connected to a smartphone via an inbuilt Wi-Fi that allowed the user to establish the connection to control the LAMP box. A 5 V USB power source was used as a power supply. The sensitivity of the LAMP assay was estimated to be up to 10(−6) dilution limit using the amplified, purified, and quantified specific DNA templates. It can also serve as an efficient diagnostic platform for many other veterinary infectious or parasitic diseases of zoonotic origin majorly towards field-based diagnostics. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00436-021-07077-z. Springer Berlin Heidelberg 2021-03-02 2021 /pmc/articles/PMC7920752/ /pubmed/33649963 http://dx.doi.org/10.1007/s00436-021-07077-z Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Protozoology - Original Paper Upadhyay, Archana Waleed, Raza Muhammad Wang, Jinhua Zhao, Jianguo Guan, Qingfeng Liao, Chenghong Han, Qian An innovative and user-friendly smartphone-assisted molecular diagnostic approach for rapid detection of canine vector-borne diseases |
title | An innovative and user-friendly smartphone-assisted molecular diagnostic approach for rapid detection of canine vector-borne diseases |
title_full | An innovative and user-friendly smartphone-assisted molecular diagnostic approach for rapid detection of canine vector-borne diseases |
title_fullStr | An innovative and user-friendly smartphone-assisted molecular diagnostic approach for rapid detection of canine vector-borne diseases |
title_full_unstemmed | An innovative and user-friendly smartphone-assisted molecular diagnostic approach for rapid detection of canine vector-borne diseases |
title_short | An innovative and user-friendly smartphone-assisted molecular diagnostic approach for rapid detection of canine vector-borne diseases |
title_sort | innovative and user-friendly smartphone-assisted molecular diagnostic approach for rapid detection of canine vector-borne diseases |
topic | Protozoology - Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920752/ https://www.ncbi.nlm.nih.gov/pubmed/33649963 http://dx.doi.org/10.1007/s00436-021-07077-z |
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