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Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae
Glanders, caused by Burkholderia (B.) mallei is a notifiable zoonotic disease in equidae. For international trade and movement of equids, certificates of negative serological test results for antibodies against B. mallei are required. To date, the complement fixation test (CFT) is the mandatory test...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920956/ https://www.ncbi.nlm.nih.gov/pubmed/33665218 http://dx.doi.org/10.3389/fvets.2021.628389 |
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author | Elschner, Mandy Carolina Melzer, Falk Singha, Harisankar Muhammad, Saqib Gardner, Ian Neubauer, Heinrich |
author_facet | Elschner, Mandy Carolina Melzer, Falk Singha, Harisankar Muhammad, Saqib Gardner, Ian Neubauer, Heinrich |
author_sort | Elschner, Mandy Carolina |
collection | PubMed |
description | Glanders, caused by Burkholderia (B.) mallei is a notifiable zoonotic disease in equidae. For international trade and movement of equids, certificates of negative serological test results for antibodies against B. mallei are required. To date, the complement fixation test (CFT) is the mandatory test to issue these health certificates. The CFT is difficult to standardize and, due to its poor specificity, often leads to false-positive reactions resulting in trade restrictions with considerable financial consequences. In the present study, the new ID Screen Glanders Double Antigen Multispecies ELISA (GLANDA- ELISA) (IDvet, Grabels, France) was evaluated using 400 negative and 370 glanders positive field samples of equidae. The GLANDA-ELISA was significantly more specific (99.8%) than the CFT (97.0%). Considering the comparable sensitivities of CFT (96.5%) and ELISA (98.1%), this new GLANDA-ELISA test appears a suitable confirmatory test and a realistic alternative for serological testing of horses for trade or movement. |
format | Online Article Text |
id | pubmed-7920956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79209562021-03-03 Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae Elschner, Mandy Carolina Melzer, Falk Singha, Harisankar Muhammad, Saqib Gardner, Ian Neubauer, Heinrich Front Vet Sci Veterinary Science Glanders, caused by Burkholderia (B.) mallei is a notifiable zoonotic disease in equidae. For international trade and movement of equids, certificates of negative serological test results for antibodies against B. mallei are required. To date, the complement fixation test (CFT) is the mandatory test to issue these health certificates. The CFT is difficult to standardize and, due to its poor specificity, often leads to false-positive reactions resulting in trade restrictions with considerable financial consequences. In the present study, the new ID Screen Glanders Double Antigen Multispecies ELISA (GLANDA- ELISA) (IDvet, Grabels, France) was evaluated using 400 negative and 370 glanders positive field samples of equidae. The GLANDA-ELISA was significantly more specific (99.8%) than the CFT (97.0%). Considering the comparable sensitivities of CFT (96.5%) and ELISA (98.1%), this new GLANDA-ELISA test appears a suitable confirmatory test and a realistic alternative for serological testing of horses for trade or movement. Frontiers Media S.A. 2021-02-16 /pmc/articles/PMC7920956/ /pubmed/33665218 http://dx.doi.org/10.3389/fvets.2021.628389 Text en Copyright © 2021 Elschner, Melzer, Singha, Muhammad, Gardner and Neubauer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Elschner, Mandy Carolina Melzer, Falk Singha, Harisankar Muhammad, Saqib Gardner, Ian Neubauer, Heinrich Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae |
title | Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae |
title_full | Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae |
title_fullStr | Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae |
title_full_unstemmed | Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae |
title_short | Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae |
title_sort | validation of a commercial glanders elisa as an alternative to the cft in international trade of equidae |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920956/ https://www.ncbi.nlm.nih.gov/pubmed/33665218 http://dx.doi.org/10.3389/fvets.2021.628389 |
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