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Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae

Glanders, caused by Burkholderia (B.) mallei is a notifiable zoonotic disease in equidae. For international trade and movement of equids, certificates of negative serological test results for antibodies against B. mallei are required. To date, the complement fixation test (CFT) is the mandatory test...

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Autores principales: Elschner, Mandy Carolina, Melzer, Falk, Singha, Harisankar, Muhammad, Saqib, Gardner, Ian, Neubauer, Heinrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920956/
https://www.ncbi.nlm.nih.gov/pubmed/33665218
http://dx.doi.org/10.3389/fvets.2021.628389
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author Elschner, Mandy Carolina
Melzer, Falk
Singha, Harisankar
Muhammad, Saqib
Gardner, Ian
Neubauer, Heinrich
author_facet Elschner, Mandy Carolina
Melzer, Falk
Singha, Harisankar
Muhammad, Saqib
Gardner, Ian
Neubauer, Heinrich
author_sort Elschner, Mandy Carolina
collection PubMed
description Glanders, caused by Burkholderia (B.) mallei is a notifiable zoonotic disease in equidae. For international trade and movement of equids, certificates of negative serological test results for antibodies against B. mallei are required. To date, the complement fixation test (CFT) is the mandatory test to issue these health certificates. The CFT is difficult to standardize and, due to its poor specificity, often leads to false-positive reactions resulting in trade restrictions with considerable financial consequences. In the present study, the new ID Screen Glanders Double Antigen Multispecies ELISA (GLANDA- ELISA) (IDvet, Grabels, France) was evaluated using 400 negative and 370 glanders positive field samples of equidae. The GLANDA-ELISA was significantly more specific (99.8%) than the CFT (97.0%). Considering the comparable sensitivities of CFT (96.5%) and ELISA (98.1%), this new GLANDA-ELISA test appears a suitable confirmatory test and a realistic alternative for serological testing of horses for trade or movement.
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spelling pubmed-79209562021-03-03 Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae Elschner, Mandy Carolina Melzer, Falk Singha, Harisankar Muhammad, Saqib Gardner, Ian Neubauer, Heinrich Front Vet Sci Veterinary Science Glanders, caused by Burkholderia (B.) mallei is a notifiable zoonotic disease in equidae. For international trade and movement of equids, certificates of negative serological test results for antibodies against B. mallei are required. To date, the complement fixation test (CFT) is the mandatory test to issue these health certificates. The CFT is difficult to standardize and, due to its poor specificity, often leads to false-positive reactions resulting in trade restrictions with considerable financial consequences. In the present study, the new ID Screen Glanders Double Antigen Multispecies ELISA (GLANDA- ELISA) (IDvet, Grabels, France) was evaluated using 400 negative and 370 glanders positive field samples of equidae. The GLANDA-ELISA was significantly more specific (99.8%) than the CFT (97.0%). Considering the comparable sensitivities of CFT (96.5%) and ELISA (98.1%), this new GLANDA-ELISA test appears a suitable confirmatory test and a realistic alternative for serological testing of horses for trade or movement. Frontiers Media S.A. 2021-02-16 /pmc/articles/PMC7920956/ /pubmed/33665218 http://dx.doi.org/10.3389/fvets.2021.628389 Text en Copyright © 2021 Elschner, Melzer, Singha, Muhammad, Gardner and Neubauer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Elschner, Mandy Carolina
Melzer, Falk
Singha, Harisankar
Muhammad, Saqib
Gardner, Ian
Neubauer, Heinrich
Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae
title Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae
title_full Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae
title_fullStr Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae
title_full_unstemmed Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae
title_short Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae
title_sort validation of a commercial glanders elisa as an alternative to the cft in international trade of equidae
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7920956/
https://www.ncbi.nlm.nih.gov/pubmed/33665218
http://dx.doi.org/10.3389/fvets.2021.628389
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