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Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in the mouse...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921133/ https://www.ncbi.nlm.nih.gov/pubmed/33649472 http://dx.doi.org/10.1038/s41598-021-84210-z |
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author | Hamabe-Horiike, Toshihide Kawasaki, Kanji Sakashita, Masataka Ishizu, Chihiro Yoshizaki, Tomokazu Harada, Shin-ichi Ogawa-Ochiai, Keiko Shinmyo, Yohei Kawasaki, Hiroshi |
author_facet | Hamabe-Horiike, Toshihide Kawasaki, Kanji Sakashita, Masataka Ishizu, Chihiro Yoshizaki, Tomokazu Harada, Shin-ichi Ogawa-Ochiai, Keiko Shinmyo, Yohei Kawasaki, Hiroshi |
author_sort | Hamabe-Horiike, Toshihide |
collection | PubMed |
description | Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in the mouse cerebrum would be valuable. Here we uncovered that the Gfa2 promoter is suitable for selective gene expression in astrocytes when used with the piggyBac system and in utero electroporation. In contrast, the Blbp promoter, which has been used to induce astrocyte-specific gene expression in transgenic mice, did not result in astrocyte-specific gene expression. We also identified the Plp1 and Mbp promoters could be used with the piggyBac system and in utero electroporation to induce selective gene expression in oligodendrocytes. Furthermore, using our technique, neuron-astrocyte or neuron-oligodendrocyte interactions can be visualized by labeling neurons, astrocytes and oligodendrocytes differentially. Our study provides a fundamental basis for specific transgene expression in astrocytes and/or oligodendrocytes in the mouse cerebrum. |
format | Online Article Text |
id | pubmed-7921133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79211332021-03-02 Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation Hamabe-Horiike, Toshihide Kawasaki, Kanji Sakashita, Masataka Ishizu, Chihiro Yoshizaki, Tomokazu Harada, Shin-ichi Ogawa-Ochiai, Keiko Shinmyo, Yohei Kawasaki, Hiroshi Sci Rep Article Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in the mouse cerebrum would be valuable. Here we uncovered that the Gfa2 promoter is suitable for selective gene expression in astrocytes when used with the piggyBac system and in utero electroporation. In contrast, the Blbp promoter, which has been used to induce astrocyte-specific gene expression in transgenic mice, did not result in astrocyte-specific gene expression. We also identified the Plp1 and Mbp promoters could be used with the piggyBac system and in utero electroporation to induce selective gene expression in oligodendrocytes. Furthermore, using our technique, neuron-astrocyte or neuron-oligodendrocyte interactions can be visualized by labeling neurons, astrocytes and oligodendrocytes differentially. Our study provides a fundamental basis for specific transgene expression in astrocytes and/or oligodendrocytes in the mouse cerebrum. Nature Publishing Group UK 2021-03-01 /pmc/articles/PMC7921133/ /pubmed/33649472 http://dx.doi.org/10.1038/s41598-021-84210-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Hamabe-Horiike, Toshihide Kawasaki, Kanji Sakashita, Masataka Ishizu, Chihiro Yoshizaki, Tomokazu Harada, Shin-ichi Ogawa-Ochiai, Keiko Shinmyo, Yohei Kawasaki, Hiroshi Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation |
title | Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation |
title_full | Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation |
title_fullStr | Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation |
title_full_unstemmed | Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation |
title_short | Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation |
title_sort | glial cell type-specific gene expression in the mouse cerebrum using the piggybac system and in utero electroporation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921133/ https://www.ncbi.nlm.nih.gov/pubmed/33649472 http://dx.doi.org/10.1038/s41598-021-84210-z |
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