Cargando…

Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation

Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in the mouse...

Descripción completa

Detalles Bibliográficos
Autores principales: Hamabe-Horiike, Toshihide, Kawasaki, Kanji, Sakashita, Masataka, Ishizu, Chihiro, Yoshizaki, Tomokazu, Harada, Shin-ichi, Ogawa-Ochiai, Keiko, Shinmyo, Yohei, Kawasaki, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921133/
https://www.ncbi.nlm.nih.gov/pubmed/33649472
http://dx.doi.org/10.1038/s41598-021-84210-z
_version_ 1783658414345289728
author Hamabe-Horiike, Toshihide
Kawasaki, Kanji
Sakashita, Masataka
Ishizu, Chihiro
Yoshizaki, Tomokazu
Harada, Shin-ichi
Ogawa-Ochiai, Keiko
Shinmyo, Yohei
Kawasaki, Hiroshi
author_facet Hamabe-Horiike, Toshihide
Kawasaki, Kanji
Sakashita, Masataka
Ishizu, Chihiro
Yoshizaki, Tomokazu
Harada, Shin-ichi
Ogawa-Ochiai, Keiko
Shinmyo, Yohei
Kawasaki, Hiroshi
author_sort Hamabe-Horiike, Toshihide
collection PubMed
description Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in the mouse cerebrum would be valuable. Here we uncovered that the Gfa2 promoter is suitable for selective gene expression in astrocytes when used with the piggyBac system and in utero electroporation. In contrast, the Blbp promoter, which has been used to induce astrocyte-specific gene expression in transgenic mice, did not result in astrocyte-specific gene expression. We also identified the Plp1 and Mbp promoters could be used with the piggyBac system and in utero electroporation to induce selective gene expression in oligodendrocytes. Furthermore, using our technique, neuron-astrocyte or neuron-oligodendrocyte interactions can be visualized by labeling neurons, astrocytes and oligodendrocytes differentially. Our study provides a fundamental basis for specific transgene expression in astrocytes and/or oligodendrocytes in the mouse cerebrum.
format Online
Article
Text
id pubmed-7921133
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-79211332021-03-02 Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation Hamabe-Horiike, Toshihide Kawasaki, Kanji Sakashita, Masataka Ishizu, Chihiro Yoshizaki, Tomokazu Harada, Shin-ichi Ogawa-Ochiai, Keiko Shinmyo, Yohei Kawasaki, Hiroshi Sci Rep Article Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in the mouse cerebrum would be valuable. Here we uncovered that the Gfa2 promoter is suitable for selective gene expression in astrocytes when used with the piggyBac system and in utero electroporation. In contrast, the Blbp promoter, which has been used to induce astrocyte-specific gene expression in transgenic mice, did not result in astrocyte-specific gene expression. We also identified the Plp1 and Mbp promoters could be used with the piggyBac system and in utero electroporation to induce selective gene expression in oligodendrocytes. Furthermore, using our technique, neuron-astrocyte or neuron-oligodendrocyte interactions can be visualized by labeling neurons, astrocytes and oligodendrocytes differentially. Our study provides a fundamental basis for specific transgene expression in astrocytes and/or oligodendrocytes in the mouse cerebrum. Nature Publishing Group UK 2021-03-01 /pmc/articles/PMC7921133/ /pubmed/33649472 http://dx.doi.org/10.1038/s41598-021-84210-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hamabe-Horiike, Toshihide
Kawasaki, Kanji
Sakashita, Masataka
Ishizu, Chihiro
Yoshizaki, Tomokazu
Harada, Shin-ichi
Ogawa-Ochiai, Keiko
Shinmyo, Yohei
Kawasaki, Hiroshi
Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
title Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
title_full Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
title_fullStr Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
title_full_unstemmed Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
title_short Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation
title_sort glial cell type-specific gene expression in the mouse cerebrum using the piggybac system and in utero electroporation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921133/
https://www.ncbi.nlm.nih.gov/pubmed/33649472
http://dx.doi.org/10.1038/s41598-021-84210-z
work_keys_str_mv AT hamabehoriiketoshihide glialcelltypespecificgeneexpressioninthemousecerebrumusingthepiggybacsystemandinuteroelectroporation
AT kawasakikanji glialcelltypespecificgeneexpressioninthemousecerebrumusingthepiggybacsystemandinuteroelectroporation
AT sakashitamasataka glialcelltypespecificgeneexpressioninthemousecerebrumusingthepiggybacsystemandinuteroelectroporation
AT ishizuchihiro glialcelltypespecificgeneexpressioninthemousecerebrumusingthepiggybacsystemandinuteroelectroporation
AT yoshizakitomokazu glialcelltypespecificgeneexpressioninthemousecerebrumusingthepiggybacsystemandinuteroelectroporation
AT haradashinichi glialcelltypespecificgeneexpressioninthemousecerebrumusingthepiggybacsystemandinuteroelectroporation
AT ogawaochiaikeiko glialcelltypespecificgeneexpressioninthemousecerebrumusingthepiggybacsystemandinuteroelectroporation
AT shinmyoyohei glialcelltypespecificgeneexpressioninthemousecerebrumusingthepiggybacsystemandinuteroelectroporation
AT kawasakihiroshi glialcelltypespecificgeneexpressioninthemousecerebrumusingthepiggybacsystemandinuteroelectroporation