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Endogenous 2μ Plasmid Editing for Pathway Engineering in Saccharomyces cerevisiae

In Saccharomyces cerevisiae, conventional 2μ-plasmid based plasmid (pC2μ, such as pRS425) have been widely adopted in pathway engineering for multi-copy overexpression of key genes. However, the loss of partition and copy number control elements of yeast endogenous 2μ plasmid (pE2μ) brings the issue...

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Detalles Bibliográficos
Autores principales:	Zeng, Bo-Xuan, Yao, Ming-Dong, Xiao, Wen-Hai, Luo, Yun-Zi, Wang, Ying, Yuan, Ying-Jin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921170/ https://www.ncbi.nlm.nih.gov/pubmed/33664720 http://dx.doi.org/10.3389/fmicb.2021.631462

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