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Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats

Caseous lymphadenitis (CLA) is a chronic disease that affects small ruminants and causes economic losses in the associated breeding system. The causative agent of CLA is Corynebacterium pseudotuberculosis, a Gram-positive bacterium that exhibits tropism for external and internal lymph nodes and indu...

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Autores principales: de Sá, Maria Conceição Aquino, da Silva, Wanderson Marques, Rodrigues, Carla Catarine Santos, Rezende, Cristiana Perdigão, Marchioro, Silvana Beutinger, Rocha Filho, José Tadeu Raynal, Sousa, Thiago de Jesus, de Oliveira, Helinando Pequeno, da Costa, Mateus Matiuzzi, Figueiredo, Henrique César Pereira, Portela, Ricardo Dias, Castro, Thiago Luiz de Paula, Azevedo, Vasco, Seyffert, Nubia, Meyer, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921313/
https://www.ncbi.nlm.nih.gov/pubmed/33665217
http://dx.doi.org/10.3389/fvets.2021.614011
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author de Sá, Maria Conceição Aquino
da Silva, Wanderson Marques
Rodrigues, Carla Catarine Santos
Rezende, Cristiana Perdigão
Marchioro, Silvana Beutinger
Rocha Filho, José Tadeu Raynal
Sousa, Thiago de Jesus
de Oliveira, Helinando Pequeno
da Costa, Mateus Matiuzzi
Figueiredo, Henrique César Pereira
Portela, Ricardo Dias
Castro, Thiago Luiz de Paula
Azevedo, Vasco
Seyffert, Nubia
Meyer, Roberto
author_facet de Sá, Maria Conceição Aquino
da Silva, Wanderson Marques
Rodrigues, Carla Catarine Santos
Rezende, Cristiana Perdigão
Marchioro, Silvana Beutinger
Rocha Filho, José Tadeu Raynal
Sousa, Thiago de Jesus
de Oliveira, Helinando Pequeno
da Costa, Mateus Matiuzzi
Figueiredo, Henrique César Pereira
Portela, Ricardo Dias
Castro, Thiago Luiz de Paula
Azevedo, Vasco
Seyffert, Nubia
Meyer, Roberto
author_sort de Sá, Maria Conceição Aquino
collection PubMed
description Caseous lymphadenitis (CLA) is a chronic disease that affects small ruminants and causes economic losses in the associated breeding system. The causative agent of CLA is Corynebacterium pseudotuberculosis, a Gram-positive bacterium that exhibits tropism for external and internal lymph nodes and induces abscess formation in the host. Bacterial communities often produce a biofilm matrix that serves various functions, including protection against hostile environmental conditions, antibiotics, and the host immune response. Although biofilm formation has been reported for C. pseudotuberculosis, not all strains demonstrate this property in culture. In this work, we report the first comparative proteomic analysis of one biofilm-forming (CAPJ4) and one biofilm-non-forming strain (CAP3W) of C. pseudotuberculosis isolated from goats. Bacterial whole cell protein extracts were obtained for mass spectrometry analyses. Using LC-MS/MS, our studies reveal three and four proteins exclusively found in the CAPJ4 and CAP3W proteome, respectively. In addition, label-free quantitative analysis identified 40 proteins showing at-least 2-fold higher values in CAPJ4 compared CAP3W proteome Notably, CAPJ4 differentially synthesized the penicillin-binding protein, which participates in the formation of peptidoglycans. CAPJ4 also exhibited upregulation of N-acetylmuramoyl-L-alanine amidase and galactose-1-phosphate uridylyltransferase, which are involved in biofilm formation and exopolysaccharide biosynthesis. Here, we demonstrate that biofilm formation in C. pseudotuberculosis is likely associated with specific proteins, some of which were previously shown to be associated with virulence and biofilm formation in other organisms. Our findings may drive studies related to the bacterial mechanisms involved in the biofilm formation, in addition to providing targets for the treatment of CLA.
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spelling pubmed-79213132021-03-03 Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats de Sá, Maria Conceição Aquino da Silva, Wanderson Marques Rodrigues, Carla Catarine Santos Rezende, Cristiana Perdigão Marchioro, Silvana Beutinger Rocha Filho, José Tadeu Raynal Sousa, Thiago de Jesus de Oliveira, Helinando Pequeno da Costa, Mateus Matiuzzi Figueiredo, Henrique César Pereira Portela, Ricardo Dias Castro, Thiago Luiz de Paula Azevedo, Vasco Seyffert, Nubia Meyer, Roberto Front Vet Sci Veterinary Science Caseous lymphadenitis (CLA) is a chronic disease that affects small ruminants and causes economic losses in the associated breeding system. The causative agent of CLA is Corynebacterium pseudotuberculosis, a Gram-positive bacterium that exhibits tropism for external and internal lymph nodes and induces abscess formation in the host. Bacterial communities often produce a biofilm matrix that serves various functions, including protection against hostile environmental conditions, antibiotics, and the host immune response. Although biofilm formation has been reported for C. pseudotuberculosis, not all strains demonstrate this property in culture. In this work, we report the first comparative proteomic analysis of one biofilm-forming (CAPJ4) and one biofilm-non-forming strain (CAP3W) of C. pseudotuberculosis isolated from goats. Bacterial whole cell protein extracts were obtained for mass spectrometry analyses. Using LC-MS/MS, our studies reveal three and four proteins exclusively found in the CAPJ4 and CAP3W proteome, respectively. In addition, label-free quantitative analysis identified 40 proteins showing at-least 2-fold higher values in CAPJ4 compared CAP3W proteome Notably, CAPJ4 differentially synthesized the penicillin-binding protein, which participates in the formation of peptidoglycans. CAPJ4 also exhibited upregulation of N-acetylmuramoyl-L-alanine amidase and galactose-1-phosphate uridylyltransferase, which are involved in biofilm formation and exopolysaccharide biosynthesis. Here, we demonstrate that biofilm formation in C. pseudotuberculosis is likely associated with specific proteins, some of which were previously shown to be associated with virulence and biofilm formation in other organisms. Our findings may drive studies related to the bacterial mechanisms involved in the biofilm formation, in addition to providing targets for the treatment of CLA. Frontiers Media S.A. 2021-02-16 /pmc/articles/PMC7921313/ /pubmed/33665217 http://dx.doi.org/10.3389/fvets.2021.614011 Text en Copyright © 2021 Sá, Silva, Rodrigues, Rezende, Marchioro, Rocha Filho, Sousa, de Oliveira, Costa, Figueiredo, Portela, Castro, Azevedo, Seyffert and Meyer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
de Sá, Maria Conceição Aquino
da Silva, Wanderson Marques
Rodrigues, Carla Catarine Santos
Rezende, Cristiana Perdigão
Marchioro, Silvana Beutinger
Rocha Filho, José Tadeu Raynal
Sousa, Thiago de Jesus
de Oliveira, Helinando Pequeno
da Costa, Mateus Matiuzzi
Figueiredo, Henrique César Pereira
Portela, Ricardo Dias
Castro, Thiago Luiz de Paula
Azevedo, Vasco
Seyffert, Nubia
Meyer, Roberto
Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats
title Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats
title_full Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats
title_fullStr Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats
title_full_unstemmed Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats
title_short Comparative Proteomic Analyses Between Biofilm-Forming and Non-biofilm-Forming Strains of Corynebacterium pseudotuberculosis Isolated From Goats
title_sort comparative proteomic analyses between biofilm-forming and non-biofilm-forming strains of corynebacterium pseudotuberculosis isolated from goats
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921313/
https://www.ncbi.nlm.nih.gov/pubmed/33665217
http://dx.doi.org/10.3389/fvets.2021.614011
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