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Increased Osteogenic Potential of Pre-Osteoblasts on Three-Dimensional Printed Scaffolds Compared to Porous Scaffolds for Bone Regeneration
BACKGROUND: One of the main challenges with conventional scaffold fabrication methods is the inability to control scaffold architecture. Recently, scaffolds with controlled shape and architecture have been fabricated using 3D-printing. Herein, we aimed to determine whether the much tighter control o...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pasteur Institute of Iran
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921523/ https://www.ncbi.nlm.nih.gov/pubmed/33461289 http://dx.doi.org/10.29252/ibj.25.2.78 |
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author | Zamani, Yasaman Amoabediny, Ghassem Mohammadi, Javad Zandieh-Doulabi, Behrouz Klein-Nulend, Jenneke Helder, Marco N. |
author_facet | Zamani, Yasaman Amoabediny, Ghassem Mohammadi, Javad Zandieh-Doulabi, Behrouz Klein-Nulend, Jenneke Helder, Marco N. |
author_sort | Zamani, Yasaman |
collection | PubMed |
description | BACKGROUND: One of the main challenges with conventional scaffold fabrication methods is the inability to control scaffold architecture. Recently, scaffolds with controlled shape and architecture have been fabricated using 3D-printing. Herein, we aimed to determine whether the much tighter control of microstructure of 3DP PLGA/β-TCP scaffolds is more effective in promoting osteogenesis than porous scaffolds produced by solvent casting/porogen leaching. METHODS: Physical and mechanical properties of porous and 3DP scaffolds were studied. The response of pre-osteoblasts to the scaffolds was analyzed after 14 days. RESULTS: The 3DP scaffolds had a smoother surface (R(a): 22 ± 3 µm) relative to the highly rough surface of porous scaffolds (R(a): 110 ± 15 µm). Water contact angle was 112 ± 4° on porous and 76 ± 6° on 3DP scaffolds. Porous and 3DP scaffolds had the pore size of 408 ± 90 and 315 ± 17 µm and porosity of 85 ± 5% and 39 ± 7%, respectively. Compressive strength of 3DP scaffolds (4.0 ± 0.3 MPa) was higher than porous scaffolds (1.7 ± 0.2 MPa). Collagenous matrix deposition was similar on both scaffolds. Cells proliferated from day 1 to day 14 by fourfold in porous and by 3.8-fold in 3DP scaffolds. ALP activity was 21-fold higher in 3DP scaffolds than porous scaffolds. CONCLUSION: The 3DP scaffolds show enhanced mechanical properties and ALP activity compared to porous scaffolds in vitro, suggesting that 3DP PLGA/β-TCP scaffolds are possibly more favorable for bone formation. |
format | Online Article Text |
id | pubmed-7921523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Pasteur Institute of Iran |
record_format | MEDLINE/PubMed |
spelling | pubmed-79215232021-03-11 Increased Osteogenic Potential of Pre-Osteoblasts on Three-Dimensional Printed Scaffolds Compared to Porous Scaffolds for Bone Regeneration Zamani, Yasaman Amoabediny, Ghassem Mohammadi, Javad Zandieh-Doulabi, Behrouz Klein-Nulend, Jenneke Helder, Marco N. Iran Biomed J Full Length BACKGROUND: One of the main challenges with conventional scaffold fabrication methods is the inability to control scaffold architecture. Recently, scaffolds with controlled shape and architecture have been fabricated using 3D-printing. Herein, we aimed to determine whether the much tighter control of microstructure of 3DP PLGA/β-TCP scaffolds is more effective in promoting osteogenesis than porous scaffolds produced by solvent casting/porogen leaching. METHODS: Physical and mechanical properties of porous and 3DP scaffolds were studied. The response of pre-osteoblasts to the scaffolds was analyzed after 14 days. RESULTS: The 3DP scaffolds had a smoother surface (R(a): 22 ± 3 µm) relative to the highly rough surface of porous scaffolds (R(a): 110 ± 15 µm). Water contact angle was 112 ± 4° on porous and 76 ± 6° on 3DP scaffolds. Porous and 3DP scaffolds had the pore size of 408 ± 90 and 315 ± 17 µm and porosity of 85 ± 5% and 39 ± 7%, respectively. Compressive strength of 3DP scaffolds (4.0 ± 0.3 MPa) was higher than porous scaffolds (1.7 ± 0.2 MPa). Collagenous matrix deposition was similar on both scaffolds. Cells proliferated from day 1 to day 14 by fourfold in porous and by 3.8-fold in 3DP scaffolds. ALP activity was 21-fold higher in 3DP scaffolds than porous scaffolds. CONCLUSION: The 3DP scaffolds show enhanced mechanical properties and ALP activity compared to porous scaffolds in vitro, suggesting that 3DP PLGA/β-TCP scaffolds are possibly more favorable for bone formation. Pasteur Institute of Iran 2021-03 2021-01-13 /pmc/articles/PMC7921523/ /pubmed/33461289 http://dx.doi.org/10.29252/ibj.25.2.78 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Length Zamani, Yasaman Amoabediny, Ghassem Mohammadi, Javad Zandieh-Doulabi, Behrouz Klein-Nulend, Jenneke Helder, Marco N. Increased Osteogenic Potential of Pre-Osteoblasts on Three-Dimensional Printed Scaffolds Compared to Porous Scaffolds for Bone Regeneration |
title | Increased Osteogenic Potential of Pre-Osteoblasts on Three-Dimensional Printed Scaffolds Compared to Porous Scaffolds for Bone Regeneration |
title_full | Increased Osteogenic Potential of Pre-Osteoblasts on Three-Dimensional Printed Scaffolds Compared to Porous Scaffolds for Bone Regeneration |
title_fullStr | Increased Osteogenic Potential of Pre-Osteoblasts on Three-Dimensional Printed Scaffolds Compared to Porous Scaffolds for Bone Regeneration |
title_full_unstemmed | Increased Osteogenic Potential of Pre-Osteoblasts on Three-Dimensional Printed Scaffolds Compared to Porous Scaffolds for Bone Regeneration |
title_short | Increased Osteogenic Potential of Pre-Osteoblasts on Three-Dimensional Printed Scaffolds Compared to Porous Scaffolds for Bone Regeneration |
title_sort | increased osteogenic potential of pre-osteoblasts on three-dimensional printed scaffolds compared to porous scaffolds for bone regeneration |
topic | Full Length |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921523/ https://www.ncbi.nlm.nih.gov/pubmed/33461289 http://dx.doi.org/10.29252/ibj.25.2.78 |
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