The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer

tRNA-derived fragments (tRFs) are a new classification of small non-coding RNAs (sncRNAs) derived from the specific cleavage of precursors and mature tRNAs. Accumulating recent evidence has shown that tRFs are frequently abnormal in several cancers. Nevertheless, the role of tRFs in gastric cancer a...

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Autores principales: Tong, Linhao, Zhang, Weixu, Qu, Bicheng, Zhang, Fei, Wu, Zhonghua, Shi, Jinxin, Chen, Xiaowan, Song, Yongxi, Wang, Zhenning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Oncology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921707/
https://www.ncbi.nlm.nih.gov/pubmed/33665159
http://dx.doi.org/10.3389/fonc.2020.570916
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author Tong, Linhao
Zhang, Weixu
Qu, Bicheng
Zhang, Fei
Wu, Zhonghua
Shi, Jinxin
Chen, Xiaowan
Song, Yongxi
Wang, Zhenning
author_facet Tong, Linhao
Zhang, Weixu
Qu, Bicheng
Zhang, Fei
Wu, Zhonghua
Shi, Jinxin
Chen, Xiaowan
Song, Yongxi
Wang, Zhenning
author_sort Tong, Linhao
collection PubMed
description tRNA-derived fragments (tRFs) are a new classification of small non-coding RNAs (sncRNAs) derived from the specific cleavage of precursors and mature tRNAs. Accumulating recent evidence has shown that tRFs are frequently abnormal in several cancers. Nevertheless, the role of tRFs in gastric cancer and its mechanism remain unclear. In this study, we found abnormal expression of tRF-3017A (derived from tRNA-Val-TAC) in gastric cancer tissues and cell lines and confirmed its effect on promoting the invasion and migration of gastric cancer cells through functional experiments in vitro. Analysis of clinicopathologic data showed patients with higher tRF-3017A were associated with significantly higher lymph node metastasis. Mechanistic investigation implies that tRF-3017A regulates the tumor suppressor gene NELL2 through forming the RNA-induced silencing complex (RISC) with Argonaute (AGO) proteins. In this study, we found that higher tRF-3017A were associated with significantly higher lymph node metastasis in gastric cancer patients and the tRF-3017A may play a role in promoting the migration and invasion of gastric cancer cells by silencing tumor suppressor NELL2.
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spelling pubmed-79217072021-03-03 The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer Tong, Linhao Zhang, Weixu Qu, Bicheng Zhang, Fei Wu, Zhonghua Shi, Jinxin Chen, Xiaowan Song, Yongxi Wang, Zhenning Front Oncol Oncology tRNA-derived fragments (tRFs) are a new classification of small non-coding RNAs (sncRNAs) derived from the specific cleavage of precursors and mature tRNAs. Accumulating recent evidence has shown that tRFs are frequently abnormal in several cancers. Nevertheless, the role of tRFs in gastric cancer and its mechanism remain unclear. In this study, we found abnormal expression of tRF-3017A (derived from tRNA-Val-TAC) in gastric cancer tissues and cell lines and confirmed its effect on promoting the invasion and migration of gastric cancer cells through functional experiments in vitro. Analysis of clinicopathologic data showed patients with higher tRF-3017A were associated with significantly higher lymph node metastasis. Mechanistic investigation implies that tRF-3017A regulates the tumor suppressor gene NELL2 through forming the RNA-induced silencing complex (RISC) with Argonaute (AGO) proteins. In this study, we found that higher tRF-3017A were associated with significantly higher lymph node metastasis in gastric cancer patients and the tRF-3017A may play a role in promoting the migration and invasion of gastric cancer cells by silencing tumor suppressor NELL2. Frontiers Media S.A. 2021-02-16 /pmc/articles/PMC7921707/ /pubmed/33665159 http://dx.doi.org/10.3389/fonc.2020.570916 Text en Copyright © 2021 Tong, Zhang, Qu, Zhang, Wu, Shi, Chen, Song and Wang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Tong, Linhao
Zhang, Weixu
Qu, Bicheng
Zhang, Fei
Wu, Zhonghua
Shi, Jinxin
Chen, Xiaowan
Song, Yongxi
Wang, Zhenning
The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer
title The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer
title_full The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer
title_fullStr The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer
title_full_unstemmed The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer
title_short The tRNA-Derived Fragment-3017A Promotes Metastasis by Inhibiting NELL2 in Human Gastric Cancer
title_sort trna-derived fragment-3017a promotes metastasis by inhibiting nell2 in human gastric cancer
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921707/
https://www.ncbi.nlm.nih.gov/pubmed/33665159
http://dx.doi.org/10.3389/fonc.2020.570916
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