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Analysis of epicardial genes in embryonic mouse hearts with flow cytometry

Genetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ hybridization for f...

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Detalles Bibliográficos
Autores principales: Redpath, Andia Nicole, Lupu, Irina-Elena, Smart, Nicola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921713/
https://www.ncbi.nlm.nih.gov/pubmed/33718887
http://dx.doi.org/10.1016/j.xpro.2021.100359
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author Redpath, Andia Nicole
Lupu, Irina-Elena
Smart, Nicola
author_facet Redpath, Andia Nicole
Lupu, Irina-Elena
Smart, Nicola
author_sort Redpath, Andia Nicole
collection PubMed
description Genetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ hybridization for flow cytometry to allow simultaneous analysis of multiple marker genes at the single whole-cell level, exemplified by application to the embryonic epicardium. The protocol facilitates multiplexed quantification of gene and protein expression and temporal changes across specific cell populations. For complete details on the use and execution of this protocol, please refer to Lupu et al. (2020).
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spelling pubmed-79217132021-03-12 Analysis of epicardial genes in embryonic mouse hearts with flow cytometry Redpath, Andia Nicole Lupu, Irina-Elena Smart, Nicola STAR Protoc Protocol Genetic markers used to define discrete cell populations are seldom expressed exclusively in the population of interest and are, thus, unsuitable when evaluated individually, especially in the absence of spatial and morphological information. Here, we present fluorescence in situ hybridization for flow cytometry to allow simultaneous analysis of multiple marker genes at the single whole-cell level, exemplified by application to the embryonic epicardium. The protocol facilitates multiplexed quantification of gene and protein expression and temporal changes across specific cell populations. For complete details on the use and execution of this protocol, please refer to Lupu et al. (2020). Elsevier 2021-02-24 /pmc/articles/PMC7921713/ /pubmed/33718887 http://dx.doi.org/10.1016/j.xpro.2021.100359 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Redpath, Andia Nicole
Lupu, Irina-Elena
Smart, Nicola
Analysis of epicardial genes in embryonic mouse hearts with flow cytometry
title Analysis of epicardial genes in embryonic mouse hearts with flow cytometry
title_full Analysis of epicardial genes in embryonic mouse hearts with flow cytometry
title_fullStr Analysis of epicardial genes in embryonic mouse hearts with flow cytometry
title_full_unstemmed Analysis of epicardial genes in embryonic mouse hearts with flow cytometry
title_short Analysis of epicardial genes in embryonic mouse hearts with flow cytometry
title_sort analysis of epicardial genes in embryonic mouse hearts with flow cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921713/
https://www.ncbi.nlm.nih.gov/pubmed/33718887
http://dx.doi.org/10.1016/j.xpro.2021.100359
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