Genetic Diversity and Potential Paths of Transmission of Mycobacterium bovis in the Amazon: The Discovery of M. bovis Lineage Lb1 Circulating in South America

Bovine tuberculosis (bTB) has yet to be eradicated in Brazil. Herds of cattle and buffalo are important sources of revenue to people living in the banks of the Amazon River basin. A better understanding of Mycobacterium bovis (M. bovis) populational structure and transmission dynamics affecting thes...

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Autores principales: Carneiro, Paulo Alex, Zimpel, Cristina Kraemer, Pasquatti, Taynara Nunes, Silva-Pereira, Taiana T., Takatani, Haruo, Silva, Christian B. D. G., Abramovitch, Robert B., Sa Guimaraes, Ana Marcia, Davila, Alberto M. R., Araujo, Flabio R., Kaneene, John B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921743/
https://www.ncbi.nlm.nih.gov/pubmed/33665220
http://dx.doi.org/10.3389/fvets.2021.630989
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author Carneiro, Paulo Alex
Zimpel, Cristina Kraemer
Pasquatti, Taynara Nunes
Silva-Pereira, Taiana T.
Takatani, Haruo
Silva, Christian B. D. G.
Abramovitch, Robert B.
Sa Guimaraes, Ana Marcia
Davila, Alberto M. R.
Araujo, Flabio R.
Kaneene, John B.
author_facet Carneiro, Paulo Alex
Zimpel, Cristina Kraemer
Pasquatti, Taynara Nunes
Silva-Pereira, Taiana T.
Takatani, Haruo
Silva, Christian B. D. G.
Abramovitch, Robert B.
Sa Guimaraes, Ana Marcia
Davila, Alberto M. R.
Araujo, Flabio R.
Kaneene, John B.
author_sort Carneiro, Paulo Alex
collection PubMed
description Bovine tuberculosis (bTB) has yet to be eradicated in Brazil. Herds of cattle and buffalo are important sources of revenue to people living in the banks of the Amazon River basin. A better understanding of Mycobacterium bovis (M. bovis) populational structure and transmission dynamics affecting these animals can significantly contribute in efforts to improve their sanitary status. Herein, we sequenced the whole genome of 22 M. bovis isolates (15 from buffalo and 7 from cattle) from 10 municipalities in the region of the Lower Amazon River Basin in Brazil and performed phylogenomic analysis and Single Nucleotide Polymorphism (SNP)-based transmission inference to evaluate population structure and transmission networks. Additionally, we compared these genomes to others obtained in unrelated studies in the Marajó Island (n = 15) and worldwide (n = 128) to understand strain diversity in the Amazon and to infer M. bovis lineages. Our results show a higher genomic diversity of M. bovis genomes obtained in the Lower Amazon River region when compared to the Marajó Island, while no significant difference was observed between M. bovis genomes obtained from cattle and buffalo (p ≥ 0.05). This high genetic diversity is reflected by the weak phylogenetic clustering of M. bovis from the Lower Amazon River region based on geographic proximity and in the detection of only two putative transmission clusters in the region. One of these clusters is the first description of inter-species transmission between cattle and buffalo in the Amazon, bringing implications to the bTB control program. Surprisingly, two M. bovis lineages were detected in our dataset, namely Lb1 and Lb3, constituting the first description of Lb1 in South America. Most of the strains of this study (13/22) and all 15 strains of the Marajó Island carried no clonal complex marker, suggesting that the recent lineage classification better describe the diversity of M. bovis in the Amazon.
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spelling pubmed-79217432021-03-03 Genetic Diversity and Potential Paths of Transmission of Mycobacterium bovis in the Amazon: The Discovery of M. bovis Lineage Lb1 Circulating in South America Carneiro, Paulo Alex Zimpel, Cristina Kraemer Pasquatti, Taynara Nunes Silva-Pereira, Taiana T. Takatani, Haruo Silva, Christian B. D. G. Abramovitch, Robert B. Sa Guimaraes, Ana Marcia Davila, Alberto M. R. Araujo, Flabio R. Kaneene, John B. Front Vet Sci Veterinary Science Bovine tuberculosis (bTB) has yet to be eradicated in Brazil. Herds of cattle and buffalo are important sources of revenue to people living in the banks of the Amazon River basin. A better understanding of Mycobacterium bovis (M. bovis) populational structure and transmission dynamics affecting these animals can significantly contribute in efforts to improve their sanitary status. Herein, we sequenced the whole genome of 22 M. bovis isolates (15 from buffalo and 7 from cattle) from 10 municipalities in the region of the Lower Amazon River Basin in Brazil and performed phylogenomic analysis and Single Nucleotide Polymorphism (SNP)-based transmission inference to evaluate population structure and transmission networks. Additionally, we compared these genomes to others obtained in unrelated studies in the Marajó Island (n = 15) and worldwide (n = 128) to understand strain diversity in the Amazon and to infer M. bovis lineages. Our results show a higher genomic diversity of M. bovis genomes obtained in the Lower Amazon River region when compared to the Marajó Island, while no significant difference was observed between M. bovis genomes obtained from cattle and buffalo (p ≥ 0.05). This high genetic diversity is reflected by the weak phylogenetic clustering of M. bovis from the Lower Amazon River region based on geographic proximity and in the detection of only two putative transmission clusters in the region. One of these clusters is the first description of inter-species transmission between cattle and buffalo in the Amazon, bringing implications to the bTB control program. Surprisingly, two M. bovis lineages were detected in our dataset, namely Lb1 and Lb3, constituting the first description of Lb1 in South America. Most of the strains of this study (13/22) and all 15 strains of the Marajó Island carried no clonal complex marker, suggesting that the recent lineage classification better describe the diversity of M. bovis in the Amazon. Frontiers Media S.A. 2021-02-16 /pmc/articles/PMC7921743/ /pubmed/33665220 http://dx.doi.org/10.3389/fvets.2021.630989 Text en Copyright © 2021 Carneiro, Zimpel, Pasquatti, Silva-Pereira, Takatani, Silva, Abramovitch, Sa Guimaraes, Davila, Araujo and Kaneene. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Carneiro, Paulo Alex
Zimpel, Cristina Kraemer
Pasquatti, Taynara Nunes
Silva-Pereira, Taiana T.
Takatani, Haruo
Silva, Christian B. D. G.
Abramovitch, Robert B.
Sa Guimaraes, Ana Marcia
Davila, Alberto M. R.
Araujo, Flabio R.
Kaneene, John B.
Genetic Diversity and Potential Paths of Transmission of Mycobacterium bovis in the Amazon: The Discovery of M. bovis Lineage Lb1 Circulating in South America
title Genetic Diversity and Potential Paths of Transmission of Mycobacterium bovis in the Amazon: The Discovery of M. bovis Lineage Lb1 Circulating in South America
title_full Genetic Diversity and Potential Paths of Transmission of Mycobacterium bovis in the Amazon: The Discovery of M. bovis Lineage Lb1 Circulating in South America
title_fullStr Genetic Diversity and Potential Paths of Transmission of Mycobacterium bovis in the Amazon: The Discovery of M. bovis Lineage Lb1 Circulating in South America
title_full_unstemmed Genetic Diversity and Potential Paths of Transmission of Mycobacterium bovis in the Amazon: The Discovery of M. bovis Lineage Lb1 Circulating in South America
title_short Genetic Diversity and Potential Paths of Transmission of Mycobacterium bovis in the Amazon: The Discovery of M. bovis Lineage Lb1 Circulating in South America
title_sort genetic diversity and potential paths of transmission of mycobacterium bovis in the amazon: the discovery of m. bovis lineage lb1 circulating in south america
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921743/
https://www.ncbi.nlm.nih.gov/pubmed/33665220
http://dx.doi.org/10.3389/fvets.2021.630989
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