Cargando…
Therapeutic Potentials of Syzygium fruticosum Fruit (Seed) Reflected into an Array of Pharmacological Assays and Prospective Receptors-Mediated Pathways
Syzygium fruticosum (SF), a valuable Bangladeshi fruit, is considered an alternative therapeutic agent. Mainly, seeds are used as nutritional phytotherapy to ease physical and mental status by preventing chronic diseases. Here, we scrutinized the S. fruticosum seed’s fundamental importance in tradit...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921944/ https://www.ncbi.nlm.nih.gov/pubmed/33671381 http://dx.doi.org/10.3390/life11020155 |
_version_ | 1783658574917926912 |
---|---|
author | Moni, Jannatul Nasma Rupa Adnan, Md. Tareq, Abu Montakim Kabir, Md. Imtiazul Reza, A.S.M. Ali Nasrin, Mst. Samima Chowdhury, Kamrul Hasan Sayem, Syed Al Jawad Rahman, Md Atiar Alam, AHM Khurshid Alam, Seema Binte Sakib, Mahfuz Ahmed Oh, Ki Kwang Cho, Dong Ha Capasso, Raffaele |
author_facet | Moni, Jannatul Nasma Rupa Adnan, Md. Tareq, Abu Montakim Kabir, Md. Imtiazul Reza, A.S.M. Ali Nasrin, Mst. Samima Chowdhury, Kamrul Hasan Sayem, Syed Al Jawad Rahman, Md Atiar Alam, AHM Khurshid Alam, Seema Binte Sakib, Mahfuz Ahmed Oh, Ki Kwang Cho, Dong Ha Capasso, Raffaele |
author_sort | Moni, Jannatul Nasma Rupa |
collection | PubMed |
description | Syzygium fruticosum (SF), a valuable Bangladeshi fruit, is considered an alternative therapeutic agent. Mainly, seeds are used as nutritional phytotherapy to ease physical and mental status by preventing chronic diseases. Here, we scrutinized the S. fruticosum seed’s fundamental importance in traditional medicine by following an integrated approach combining in vivo, in vitro, and in silico studies. The SF was fractionated with different solvents, and the ethyl acetate fraction of SF (EaF-SF) was further studied. Mice treated with EaF-SF (200 and 400 mg/kg) manifested anxiolysis evidenced by higher exploration in elevated plus maze and hole board tests. Similarly, a dose-dependent drop of immobility time in a forced swimming test ensured significant anti-depressant activity. Moreover, higher dose treatment exposed reduced exploratory behaviour resembling decreased movement and prolonged sleeping latency with a quick onset of sleep during the open field and thiopental-induced sleeping tests, respectively. In parallel, EaF-SF significantly (p < 0.001) and dose-dependently suppressed acetic acid and formalin-induced pain in mice. Also, a noteworthy anti-inflammatory activity and a substantial (p < 0.01) clot lysis activity (thrombolytic) was observed. Gas chromatography-mass spectrometry (GC–MS) analysis resulted in 49 bioactive compounds. Among them, 12 bioactive compounds with Lipinski’s rule and safety confirmation showed strong binding affinity (molecular docking) against the receptors of each model used. To conclude, the S. fruticosum seed is a prospective source of health-promoting effects that can be an excellent candidate for preventing degenerative diseases. |
format | Online Article Text |
id | pubmed-7921944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79219442021-03-03 Therapeutic Potentials of Syzygium fruticosum Fruit (Seed) Reflected into an Array of Pharmacological Assays and Prospective Receptors-Mediated Pathways Moni, Jannatul Nasma Rupa Adnan, Md. Tareq, Abu Montakim Kabir, Md. Imtiazul Reza, A.S.M. Ali Nasrin, Mst. Samima Chowdhury, Kamrul Hasan Sayem, Syed Al Jawad Rahman, Md Atiar Alam, AHM Khurshid Alam, Seema Binte Sakib, Mahfuz Ahmed Oh, Ki Kwang Cho, Dong Ha Capasso, Raffaele Life (Basel) Article Syzygium fruticosum (SF), a valuable Bangladeshi fruit, is considered an alternative therapeutic agent. Mainly, seeds are used as nutritional phytotherapy to ease physical and mental status by preventing chronic diseases. Here, we scrutinized the S. fruticosum seed’s fundamental importance in traditional medicine by following an integrated approach combining in vivo, in vitro, and in silico studies. The SF was fractionated with different solvents, and the ethyl acetate fraction of SF (EaF-SF) was further studied. Mice treated with EaF-SF (200 and 400 mg/kg) manifested anxiolysis evidenced by higher exploration in elevated plus maze and hole board tests. Similarly, a dose-dependent drop of immobility time in a forced swimming test ensured significant anti-depressant activity. Moreover, higher dose treatment exposed reduced exploratory behaviour resembling decreased movement and prolonged sleeping latency with a quick onset of sleep during the open field and thiopental-induced sleeping tests, respectively. In parallel, EaF-SF significantly (p < 0.001) and dose-dependently suppressed acetic acid and formalin-induced pain in mice. Also, a noteworthy anti-inflammatory activity and a substantial (p < 0.01) clot lysis activity (thrombolytic) was observed. Gas chromatography-mass spectrometry (GC–MS) analysis resulted in 49 bioactive compounds. Among them, 12 bioactive compounds with Lipinski’s rule and safety confirmation showed strong binding affinity (molecular docking) against the receptors of each model used. To conclude, the S. fruticosum seed is a prospective source of health-promoting effects that can be an excellent candidate for preventing degenerative diseases. MDPI 2021-02-17 /pmc/articles/PMC7921944/ /pubmed/33671381 http://dx.doi.org/10.3390/life11020155 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Moni, Jannatul Nasma Rupa Adnan, Md. Tareq, Abu Montakim Kabir, Md. Imtiazul Reza, A.S.M. Ali Nasrin, Mst. Samima Chowdhury, Kamrul Hasan Sayem, Syed Al Jawad Rahman, Md Atiar Alam, AHM Khurshid Alam, Seema Binte Sakib, Mahfuz Ahmed Oh, Ki Kwang Cho, Dong Ha Capasso, Raffaele Therapeutic Potentials of Syzygium fruticosum Fruit (Seed) Reflected into an Array of Pharmacological Assays and Prospective Receptors-Mediated Pathways |
title | Therapeutic Potentials of Syzygium fruticosum Fruit (Seed) Reflected into an Array of Pharmacological Assays and Prospective Receptors-Mediated Pathways |
title_full | Therapeutic Potentials of Syzygium fruticosum Fruit (Seed) Reflected into an Array of Pharmacological Assays and Prospective Receptors-Mediated Pathways |
title_fullStr | Therapeutic Potentials of Syzygium fruticosum Fruit (Seed) Reflected into an Array of Pharmacological Assays and Prospective Receptors-Mediated Pathways |
title_full_unstemmed | Therapeutic Potentials of Syzygium fruticosum Fruit (Seed) Reflected into an Array of Pharmacological Assays and Prospective Receptors-Mediated Pathways |
title_short | Therapeutic Potentials of Syzygium fruticosum Fruit (Seed) Reflected into an Array of Pharmacological Assays and Prospective Receptors-Mediated Pathways |
title_sort | therapeutic potentials of syzygium fruticosum fruit (seed) reflected into an array of pharmacological assays and prospective receptors-mediated pathways |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7921944/ https://www.ncbi.nlm.nih.gov/pubmed/33671381 http://dx.doi.org/10.3390/life11020155 |
work_keys_str_mv | AT monijannatulnasmarupa therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT adnanmd therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT tareqabumontakim therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT kabirmdimtiazul therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT rezaasmali therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT nasrinmstsamima therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT chowdhurykamrulhasan therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT sayemsyedaljawad therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT rahmanmdatiar therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT alamahmkhurshid therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT alamseemabinte therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT sakibmahfuzahmed therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT ohkikwang therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT chodongha therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways AT capassoraffaele therapeuticpotentialsofsyzygiumfruticosumfruitseedreflectedintoanarrayofpharmacologicalassaysandprospectivereceptorsmediatedpathways |