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DNA Manipulation and Single-Molecule Imaging
DNA replication, repair, and recombination in the cell play a significant role in the regulation of the inheritance, maintenance, and transfer of genetic information. To elucidate the biomolecular mechanism in the cell, some molecular models of DNA replication, repair, and recombination have been pr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922115/ https://www.ncbi.nlm.nih.gov/pubmed/33671359 http://dx.doi.org/10.3390/molecules26041050 |
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author | Takahashi, Shunsuke Oshige, Masahiko Katsura, Shinji |
author_facet | Takahashi, Shunsuke Oshige, Masahiko Katsura, Shinji |
author_sort | Takahashi, Shunsuke |
collection | PubMed |
description | DNA replication, repair, and recombination in the cell play a significant role in the regulation of the inheritance, maintenance, and transfer of genetic information. To elucidate the biomolecular mechanism in the cell, some molecular models of DNA replication, repair, and recombination have been proposed. These biological studies have been conducted using bulk assays, such as gel electrophoresis. Because in bulk assays, several millions of biomolecules are subjected to analysis, the results of the biological analysis only reveal the average behavior of a large number of biomolecules. Therefore, revealing the elementary biological processes of a protein acting on DNA (e.g., the binding of protein to DNA, DNA synthesis, the pause of DNA synthesis, and the release of protein from DNA) is difficult. Single-molecule imaging allows the analysis of the dynamic behaviors of individual biomolecules that are hidden during bulk experiments. Thus, the methods for single-molecule imaging have provided new insights into almost all of the aspects of the elementary processes of DNA replication, repair, and recombination. However, in an aqueous solution, DNA molecules are in a randomly coiled state. Thus, the manipulation of the physical form of the single DNA molecules is important. In this review, we provide an overview of the unique studies on DNA manipulation and single-molecule imaging to analyze the dynamic interaction between DNA and protein. |
format | Online Article Text |
id | pubmed-7922115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79221152021-03-03 DNA Manipulation and Single-Molecule Imaging Takahashi, Shunsuke Oshige, Masahiko Katsura, Shinji Molecules Review DNA replication, repair, and recombination in the cell play a significant role in the regulation of the inheritance, maintenance, and transfer of genetic information. To elucidate the biomolecular mechanism in the cell, some molecular models of DNA replication, repair, and recombination have been proposed. These biological studies have been conducted using bulk assays, such as gel electrophoresis. Because in bulk assays, several millions of biomolecules are subjected to analysis, the results of the biological analysis only reveal the average behavior of a large number of biomolecules. Therefore, revealing the elementary biological processes of a protein acting on DNA (e.g., the binding of protein to DNA, DNA synthesis, the pause of DNA synthesis, and the release of protein from DNA) is difficult. Single-molecule imaging allows the analysis of the dynamic behaviors of individual biomolecules that are hidden during bulk experiments. Thus, the methods for single-molecule imaging have provided new insights into almost all of the aspects of the elementary processes of DNA replication, repair, and recombination. However, in an aqueous solution, DNA molecules are in a randomly coiled state. Thus, the manipulation of the physical form of the single DNA molecules is important. In this review, we provide an overview of the unique studies on DNA manipulation and single-molecule imaging to analyze the dynamic interaction between DNA and protein. MDPI 2021-02-17 /pmc/articles/PMC7922115/ /pubmed/33671359 http://dx.doi.org/10.3390/molecules26041050 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Takahashi, Shunsuke Oshige, Masahiko Katsura, Shinji DNA Manipulation and Single-Molecule Imaging |
title | DNA Manipulation and Single-Molecule Imaging |
title_full | DNA Manipulation and Single-Molecule Imaging |
title_fullStr | DNA Manipulation and Single-Molecule Imaging |
title_full_unstemmed | DNA Manipulation and Single-Molecule Imaging |
title_short | DNA Manipulation and Single-Molecule Imaging |
title_sort | dna manipulation and single-molecule imaging |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922115/ https://www.ncbi.nlm.nih.gov/pubmed/33671359 http://dx.doi.org/10.3390/molecules26041050 |
work_keys_str_mv | AT takahashishunsuke dnamanipulationandsinglemoleculeimaging AT oshigemasahiko dnamanipulationandsinglemoleculeimaging AT katsurashinji dnamanipulationandsinglemoleculeimaging |