Cargando…

DNA Manipulation and Single-Molecule Imaging

DNA replication, repair, and recombination in the cell play a significant role in the regulation of the inheritance, maintenance, and transfer of genetic information. To elucidate the biomolecular mechanism in the cell, some molecular models of DNA replication, repair, and recombination have been pr...

Descripción completa

Detalles Bibliográficos
Autores principales: Takahashi, Shunsuke, Oshige, Masahiko, Katsura, Shinji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922115/
https://www.ncbi.nlm.nih.gov/pubmed/33671359
http://dx.doi.org/10.3390/molecules26041050
_version_ 1783658615648813056
author Takahashi, Shunsuke
Oshige, Masahiko
Katsura, Shinji
author_facet Takahashi, Shunsuke
Oshige, Masahiko
Katsura, Shinji
author_sort Takahashi, Shunsuke
collection PubMed
description DNA replication, repair, and recombination in the cell play a significant role in the regulation of the inheritance, maintenance, and transfer of genetic information. To elucidate the biomolecular mechanism in the cell, some molecular models of DNA replication, repair, and recombination have been proposed. These biological studies have been conducted using bulk assays, such as gel electrophoresis. Because in bulk assays, several millions of biomolecules are subjected to analysis, the results of the biological analysis only reveal the average behavior of a large number of biomolecules. Therefore, revealing the elementary biological processes of a protein acting on DNA (e.g., the binding of protein to DNA, DNA synthesis, the pause of DNA synthesis, and the release of protein from DNA) is difficult. Single-molecule imaging allows the analysis of the dynamic behaviors of individual biomolecules that are hidden during bulk experiments. Thus, the methods for single-molecule imaging have provided new insights into almost all of the aspects of the elementary processes of DNA replication, repair, and recombination. However, in an aqueous solution, DNA molecules are in a randomly coiled state. Thus, the manipulation of the physical form of the single DNA molecules is important. In this review, we provide an overview of the unique studies on DNA manipulation and single-molecule imaging to analyze the dynamic interaction between DNA and protein.
format Online
Article
Text
id pubmed-7922115
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79221152021-03-03 DNA Manipulation and Single-Molecule Imaging Takahashi, Shunsuke Oshige, Masahiko Katsura, Shinji Molecules Review DNA replication, repair, and recombination in the cell play a significant role in the regulation of the inheritance, maintenance, and transfer of genetic information. To elucidate the biomolecular mechanism in the cell, some molecular models of DNA replication, repair, and recombination have been proposed. These biological studies have been conducted using bulk assays, such as gel electrophoresis. Because in bulk assays, several millions of biomolecules are subjected to analysis, the results of the biological analysis only reveal the average behavior of a large number of biomolecules. Therefore, revealing the elementary biological processes of a protein acting on DNA (e.g., the binding of protein to DNA, DNA synthesis, the pause of DNA synthesis, and the release of protein from DNA) is difficult. Single-molecule imaging allows the analysis of the dynamic behaviors of individual biomolecules that are hidden during bulk experiments. Thus, the methods for single-molecule imaging have provided new insights into almost all of the aspects of the elementary processes of DNA replication, repair, and recombination. However, in an aqueous solution, DNA molecules are in a randomly coiled state. Thus, the manipulation of the physical form of the single DNA molecules is important. In this review, we provide an overview of the unique studies on DNA manipulation and single-molecule imaging to analyze the dynamic interaction between DNA and protein. MDPI 2021-02-17 /pmc/articles/PMC7922115/ /pubmed/33671359 http://dx.doi.org/10.3390/molecules26041050 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Takahashi, Shunsuke
Oshige, Masahiko
Katsura, Shinji
DNA Manipulation and Single-Molecule Imaging
title DNA Manipulation and Single-Molecule Imaging
title_full DNA Manipulation and Single-Molecule Imaging
title_fullStr DNA Manipulation and Single-Molecule Imaging
title_full_unstemmed DNA Manipulation and Single-Molecule Imaging
title_short DNA Manipulation and Single-Molecule Imaging
title_sort dna manipulation and single-molecule imaging
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922115/
https://www.ncbi.nlm.nih.gov/pubmed/33671359
http://dx.doi.org/10.3390/molecules26041050
work_keys_str_mv AT takahashishunsuke dnamanipulationandsinglemoleculeimaging
AT oshigemasahiko dnamanipulationandsinglemoleculeimaging
AT katsurashinji dnamanipulationandsinglemoleculeimaging