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Determination of Biogenic Amines in Different Parts of Lycium barbarum L. by HPLC with Precolumn Dansylation

The aim of this work was to characterize biogenic amines (BAs) in different parts of Lycium barbarum L. using HPLC with dansyl chloride derivatization, and jointly, to provide referential data for further exploration and utilization of Lycium barbarum L. The linear correlation coefficients for all B...

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Autores principales: Ai, Yun, Sun, Yan Ni, Liu, Li, Yao, Fang Yuan, Zhang, Yan, Guo, Feng Yi, Zhao, Wen Jie, Liu, Jian Li, Zhang, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922342/
https://www.ncbi.nlm.nih.gov/pubmed/33671270
http://dx.doi.org/10.3390/molecules26041046
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author Ai, Yun
Sun, Yan Ni
Liu, Li
Yao, Fang Yuan
Zhang, Yan
Guo, Feng Yi
Zhao, Wen Jie
Liu, Jian Li
Zhang, Ning
author_facet Ai, Yun
Sun, Yan Ni
Liu, Li
Yao, Fang Yuan
Zhang, Yan
Guo, Feng Yi
Zhao, Wen Jie
Liu, Jian Li
Zhang, Ning
author_sort Ai, Yun
collection PubMed
description The aim of this work was to characterize biogenic amines (BAs) in different parts of Lycium barbarum L. using HPLC with dansyl chloride derivatization, and jointly, to provide referential data for further exploration and utilization of Lycium barbarum L. The linear correlation coefficients for all BAs were above 0.9989. The limits of detection and quantification were 0.015–0.075 and 0.05–0.25 μg/mL, respectively. The relative standard deviations for the intra-day and inter-day precision were 0.66–2.69% and 0.91–4.38%. The described method has good repeatability and intermediate precision for the quantitative determination of BAs in different parts of Lycium barbarum L. Satisfactory recovery for all amines was obtained (79.3–110.3%). The result showed that there were four kinds of BAs. The highest putrescine content (20.9 ± 3.2 mg/kg) was found in the flower. The highest histamine content (102.7 ± 5.8 mg/kg) was detected in the bark, and the highest spermidine (13.3 ± 1.6 mg/kg) and spermine (23.7 ± 2.0 mg/kg) contents were detected in the young leaves. The high histamine (HIS) content in the bark may be one of the reasons why all of the parts of Lycium barbarum L., except the bark, are used for medicine or food in China. Meanwhile, the issue of the high concentration of HIS should be considered when exploiting or utilizing the bark of Lycium barbarum L.
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spelling pubmed-79223422021-03-03 Determination of Biogenic Amines in Different Parts of Lycium barbarum L. by HPLC with Precolumn Dansylation Ai, Yun Sun, Yan Ni Liu, Li Yao, Fang Yuan Zhang, Yan Guo, Feng Yi Zhao, Wen Jie Liu, Jian Li Zhang, Ning Molecules Article The aim of this work was to characterize biogenic amines (BAs) in different parts of Lycium barbarum L. using HPLC with dansyl chloride derivatization, and jointly, to provide referential data for further exploration and utilization of Lycium barbarum L. The linear correlation coefficients for all BAs were above 0.9989. The limits of detection and quantification were 0.015–0.075 and 0.05–0.25 μg/mL, respectively. The relative standard deviations for the intra-day and inter-day precision were 0.66–2.69% and 0.91–4.38%. The described method has good repeatability and intermediate precision for the quantitative determination of BAs in different parts of Lycium barbarum L. Satisfactory recovery for all amines was obtained (79.3–110.3%). The result showed that there were four kinds of BAs. The highest putrescine content (20.9 ± 3.2 mg/kg) was found in the flower. The highest histamine content (102.7 ± 5.8 mg/kg) was detected in the bark, and the highest spermidine (13.3 ± 1.6 mg/kg) and spermine (23.7 ± 2.0 mg/kg) contents were detected in the young leaves. The high histamine (HIS) content in the bark may be one of the reasons why all of the parts of Lycium barbarum L., except the bark, are used for medicine or food in China. Meanwhile, the issue of the high concentration of HIS should be considered when exploiting or utilizing the bark of Lycium barbarum L. MDPI 2021-02-17 /pmc/articles/PMC7922342/ /pubmed/33671270 http://dx.doi.org/10.3390/molecules26041046 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ai, Yun
Sun, Yan Ni
Liu, Li
Yao, Fang Yuan
Zhang, Yan
Guo, Feng Yi
Zhao, Wen Jie
Liu, Jian Li
Zhang, Ning
Determination of Biogenic Amines in Different Parts of Lycium barbarum L. by HPLC with Precolumn Dansylation
title Determination of Biogenic Amines in Different Parts of Lycium barbarum L. by HPLC with Precolumn Dansylation
title_full Determination of Biogenic Amines in Different Parts of Lycium barbarum L. by HPLC with Precolumn Dansylation
title_fullStr Determination of Biogenic Amines in Different Parts of Lycium barbarum L. by HPLC with Precolumn Dansylation
title_full_unstemmed Determination of Biogenic Amines in Different Parts of Lycium barbarum L. by HPLC with Precolumn Dansylation
title_short Determination of Biogenic Amines in Different Parts of Lycium barbarum L. by HPLC with Precolumn Dansylation
title_sort determination of biogenic amines in different parts of lycium barbarum l. by hplc with precolumn dansylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922342/
https://www.ncbi.nlm.nih.gov/pubmed/33671270
http://dx.doi.org/10.3390/molecules26041046
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