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Characterization of Kazachstania slooffiae, a Proposed Commensal in the Porcine Gut

Kazachstania slooffiae is a fungus commonly isolated from the gastrointestinal tract and feces of post-weaning pigs. Studies have implicated its ability to positively alter piglet gut health through potential symbioses with beneficial bacteria, including Lactobacillus and Prevotella, in providing am...

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Autores principales: Summers, Katie Lynn, Foster Frey, Juli, Arfken, Ann M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922399/
https://www.ncbi.nlm.nih.gov/pubmed/33671322
http://dx.doi.org/10.3390/jof7020146
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author Summers, Katie Lynn
Foster Frey, Juli
Arfken, Ann M.
author_facet Summers, Katie Lynn
Foster Frey, Juli
Arfken, Ann M.
author_sort Summers, Katie Lynn
collection PubMed
description Kazachstania slooffiae is a fungus commonly isolated from the gastrointestinal tract and feces of post-weaning pigs. Studies have implicated its ability to positively alter piglet gut health through potential symbioses with beneficial bacteria, including Lactobacillus and Prevotella, in providing amino acids as an energy source for microbial and piglet growth, and it has been found to be positively correlated with short-chain fatty acids in the piglet gut. However, basic mycological information remains limited, hampering in vitro studies. In this study, we characterized the growth parameters, biofilm formation ability, susceptibility to antimicrobials, and genetic relatedness of K. slooffiae to other fungal isolates. Optimal fungal growth conditions were determined, no antifungal resistance was found against multiple classes of antifungal drugs (azoles, echinocandins, polyenes, or pyrimidine analogues), and dimorphic growth was observed. K. slooffiae produced biofilms that became more complex in the presence of Lactobacillus acidophilus supernatant, suggesting positive interactions with this bacterium in the gut, while Enterococcus faecalis supernatant decreased density, suggesting an antagonistic interaction. This study characterizes the in vitro growth conditions that are optimal for further studies of K. slooffiae, which is an important step in defining the role and interactions of K. slooffiae in the porcine gut environment.
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spelling pubmed-79223992021-03-03 Characterization of Kazachstania slooffiae, a Proposed Commensal in the Porcine Gut Summers, Katie Lynn Foster Frey, Juli Arfken, Ann M. J Fungi (Basel) Article Kazachstania slooffiae is a fungus commonly isolated from the gastrointestinal tract and feces of post-weaning pigs. Studies have implicated its ability to positively alter piglet gut health through potential symbioses with beneficial bacteria, including Lactobacillus and Prevotella, in providing amino acids as an energy source for microbial and piglet growth, and it has been found to be positively correlated with short-chain fatty acids in the piglet gut. However, basic mycological information remains limited, hampering in vitro studies. In this study, we characterized the growth parameters, biofilm formation ability, susceptibility to antimicrobials, and genetic relatedness of K. slooffiae to other fungal isolates. Optimal fungal growth conditions were determined, no antifungal resistance was found against multiple classes of antifungal drugs (azoles, echinocandins, polyenes, or pyrimidine analogues), and dimorphic growth was observed. K. slooffiae produced biofilms that became more complex in the presence of Lactobacillus acidophilus supernatant, suggesting positive interactions with this bacterium in the gut, while Enterococcus faecalis supernatant decreased density, suggesting an antagonistic interaction. This study characterizes the in vitro growth conditions that are optimal for further studies of K. slooffiae, which is an important step in defining the role and interactions of K. slooffiae in the porcine gut environment. MDPI 2021-02-17 /pmc/articles/PMC7922399/ /pubmed/33671322 http://dx.doi.org/10.3390/jof7020146 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Summers, Katie Lynn
Foster Frey, Juli
Arfken, Ann M.
Characterization of Kazachstania slooffiae, a Proposed Commensal in the Porcine Gut
title Characterization of Kazachstania slooffiae, a Proposed Commensal in the Porcine Gut
title_full Characterization of Kazachstania slooffiae, a Proposed Commensal in the Porcine Gut
title_fullStr Characterization of Kazachstania slooffiae, a Proposed Commensal in the Porcine Gut
title_full_unstemmed Characterization of Kazachstania slooffiae, a Proposed Commensal in the Porcine Gut
title_short Characterization of Kazachstania slooffiae, a Proposed Commensal in the Porcine Gut
title_sort characterization of kazachstania slooffiae, a proposed commensal in the porcine gut
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922399/
https://www.ncbi.nlm.nih.gov/pubmed/33671322
http://dx.doi.org/10.3390/jof7020146
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