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Identification by MALDI-TOF MS and Antibiotic Resistance of Riemerella anatipestifer, Isolated from a Clinical Case in Commercial Broiler Chickens

The Gram-negative bacterium Riemerella anatipestifer (RA) is known to cause clinical disease with severe economic impacts primarily in ducks and less frequently in geese and turkeys. RA was isolated and identified in broiler chickens, from a clinical case in a commercial broiler farm located in the...

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Autores principales: Tzora, Athina, Skoufos, Stylianos, Bonos, Eleftherios, Fotou, Konstantina, Karamoutsios, Achilleas, Nelli, Aikaterini, Giannenas, Ilias, Tsinas, Anastasios, Skoufos, Ioannis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922512/
https://www.ncbi.nlm.nih.gov/pubmed/33671477
http://dx.doi.org/10.3390/vetsci8020029
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author Tzora, Athina
Skoufos, Stylianos
Bonos, Eleftherios
Fotou, Konstantina
Karamoutsios, Achilleas
Nelli, Aikaterini
Giannenas, Ilias
Tsinas, Anastasios
Skoufos, Ioannis
author_facet Tzora, Athina
Skoufos, Stylianos
Bonos, Eleftherios
Fotou, Konstantina
Karamoutsios, Achilleas
Nelli, Aikaterini
Giannenas, Ilias
Tsinas, Anastasios
Skoufos, Ioannis
author_sort Tzora, Athina
collection PubMed
description The Gram-negative bacterium Riemerella anatipestifer (RA) is known to cause clinical disease with severe economic impacts primarily in ducks and less frequently in geese and turkeys. RA was isolated and identified in broiler chickens, from a clinical case in a commercial broiler farm located in the southwest mainland of Greece. The morbidity and the mortality in the broiler house were estimated at 10% and 5% respectively. The observed clinical signs appeared at the age of 30 to 42 days with respiratory distress (dyspnea), white fluid feces and stunting. Post-mortem examinations displayed serositis, pericarditis, perihepatitis and airsacculitis. Edematous swelling around the tibio-tarsal joints was observed in some birds. Tissue samples from lesions were streaked on selective media. Three bacterial isolates were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Moreover, an antibiogram analysis was performed for the three RA strains, using a pattern of 16 common antibiotics to advocate the most effective drugs for a proper treatment. All the RA isolates were sensitive to ceftiofur, sulphamethoxazole–trimethoprim and amoxicillin, whereas all were resistant to gentamicin, tylosin, tetracyclin, colistin sulphate, spectinomycin, lincomycin and oxytetracycline.
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spelling pubmed-79225122021-03-03 Identification by MALDI-TOF MS and Antibiotic Resistance of Riemerella anatipestifer, Isolated from a Clinical Case in Commercial Broiler Chickens Tzora, Athina Skoufos, Stylianos Bonos, Eleftherios Fotou, Konstantina Karamoutsios, Achilleas Nelli, Aikaterini Giannenas, Ilias Tsinas, Anastasios Skoufos, Ioannis Vet Sci Case Report The Gram-negative bacterium Riemerella anatipestifer (RA) is known to cause clinical disease with severe economic impacts primarily in ducks and less frequently in geese and turkeys. RA was isolated and identified in broiler chickens, from a clinical case in a commercial broiler farm located in the southwest mainland of Greece. The morbidity and the mortality in the broiler house were estimated at 10% and 5% respectively. The observed clinical signs appeared at the age of 30 to 42 days with respiratory distress (dyspnea), white fluid feces and stunting. Post-mortem examinations displayed serositis, pericarditis, perihepatitis and airsacculitis. Edematous swelling around the tibio-tarsal joints was observed in some birds. Tissue samples from lesions were streaked on selective media. Three bacterial isolates were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Moreover, an antibiogram analysis was performed for the three RA strains, using a pattern of 16 common antibiotics to advocate the most effective drugs for a proper treatment. All the RA isolates were sensitive to ceftiofur, sulphamethoxazole–trimethoprim and amoxicillin, whereas all were resistant to gentamicin, tylosin, tetracyclin, colistin sulphate, spectinomycin, lincomycin and oxytetracycline. MDPI 2021-02-17 /pmc/articles/PMC7922512/ /pubmed/33671477 http://dx.doi.org/10.3390/vetsci8020029 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Case Report
Tzora, Athina
Skoufos, Stylianos
Bonos, Eleftherios
Fotou, Konstantina
Karamoutsios, Achilleas
Nelli, Aikaterini
Giannenas, Ilias
Tsinas, Anastasios
Skoufos, Ioannis
Identification by MALDI-TOF MS and Antibiotic Resistance of Riemerella anatipestifer, Isolated from a Clinical Case in Commercial Broiler Chickens
title Identification by MALDI-TOF MS and Antibiotic Resistance of Riemerella anatipestifer, Isolated from a Clinical Case in Commercial Broiler Chickens
title_full Identification by MALDI-TOF MS and Antibiotic Resistance of Riemerella anatipestifer, Isolated from a Clinical Case in Commercial Broiler Chickens
title_fullStr Identification by MALDI-TOF MS and Antibiotic Resistance of Riemerella anatipestifer, Isolated from a Clinical Case in Commercial Broiler Chickens
title_full_unstemmed Identification by MALDI-TOF MS and Antibiotic Resistance of Riemerella anatipestifer, Isolated from a Clinical Case in Commercial Broiler Chickens
title_short Identification by MALDI-TOF MS and Antibiotic Resistance of Riemerella anatipestifer, Isolated from a Clinical Case in Commercial Broiler Chickens
title_sort identification by maldi-tof ms and antibiotic resistance of riemerella anatipestifer, isolated from a clinical case in commercial broiler chickens
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922512/
https://www.ncbi.nlm.nih.gov/pubmed/33671477
http://dx.doi.org/10.3390/vetsci8020029
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