Cargando…

Study of Sexual-Linked Genes (OGI and MeGI) on the Performance of Androecious Persimmons (Diospyros kaki Thunb.)

It is reported that the production of floral sexual phenotype in hexaploid monoecious persimmon (Diospyros kaki) is closely related to a pseudogene called OGI, and a short interspersed nuclear element (SINE)-like insertion (named Kali) in the OGI promoter leads to the gene silence. As a result, DNA...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Liyuan, Han, Weijuan, Diao, Songfeng, Suo, Yujing, Li, Huawei, Mai, Yini, Wang, Yiru, Sun, Peng, Fu, Jianmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922513/
https://www.ncbi.nlm.nih.gov/pubmed/33670570
http://dx.doi.org/10.3390/plants10020390
_version_ 1783658707446398976
author Wang, Liyuan
Han, Weijuan
Diao, Songfeng
Suo, Yujing
Li, Huawei
Mai, Yini
Wang, Yiru
Sun, Peng
Fu, Jianmin
author_facet Wang, Liyuan
Han, Weijuan
Diao, Songfeng
Suo, Yujing
Li, Huawei
Mai, Yini
Wang, Yiru
Sun, Peng
Fu, Jianmin
author_sort Wang, Liyuan
collection PubMed
description It is reported that the production of floral sexual phenotype in hexaploid monoecious persimmon (Diospyros kaki) is closely related to a pseudogene called OGI, and a short interspersed nuclear element (SINE)-like insertion (named Kali) in the OGI promoter leads to the gene silence. As a result, DNA methylation level of MeGI promoter determines the development of male or female flowers. However, the molecular mechanism in androecious D. kaki, which only bear male flowers, remains elusive. Here, real-time quantitative polymerase chain reaction (RT-qPCR), molecular cloning, and bisulfite PCR sequencing technique were carried out using 87 materials, including 56 androecious resources, 15 monoecious, and 16 gynoecious cultivars, to investigate the performance of OGI and MeGI on the specific androecious type of D. kaki in China. In conclusion, the Kali insertion was exactly located in the OGI promoter region, and the OGI gene and the Kali sequence were existing and conserved in androecious D. kaki. Meanwhile, we also demonstrated that the MeGI gene was widespread in our investigated samples. Ultimately, our result convincingly provided evidence that the low expression of OGI is probably ascribed to the presence of Kali displaying strong methylation in the OGI promoter, and low expression of MeGI, as well as high DNA methylation level, in the promoter was closely connected with the production of male flowers; this result was consistent with the monoecious persimmon model. Our findings provide predominant genetic aspects for investigation into androecious D. kaki, and future perfecting the sex-determining mechanisms in persimmon.
format Online
Article
Text
id pubmed-7922513
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79225132021-03-03 Study of Sexual-Linked Genes (OGI and MeGI) on the Performance of Androecious Persimmons (Diospyros kaki Thunb.) Wang, Liyuan Han, Weijuan Diao, Songfeng Suo, Yujing Li, Huawei Mai, Yini Wang, Yiru Sun, Peng Fu, Jianmin Plants (Basel) Article It is reported that the production of floral sexual phenotype in hexaploid monoecious persimmon (Diospyros kaki) is closely related to a pseudogene called OGI, and a short interspersed nuclear element (SINE)-like insertion (named Kali) in the OGI promoter leads to the gene silence. As a result, DNA methylation level of MeGI promoter determines the development of male or female flowers. However, the molecular mechanism in androecious D. kaki, which only bear male flowers, remains elusive. Here, real-time quantitative polymerase chain reaction (RT-qPCR), molecular cloning, and bisulfite PCR sequencing technique were carried out using 87 materials, including 56 androecious resources, 15 monoecious, and 16 gynoecious cultivars, to investigate the performance of OGI and MeGI on the specific androecious type of D. kaki in China. In conclusion, the Kali insertion was exactly located in the OGI promoter region, and the OGI gene and the Kali sequence were existing and conserved in androecious D. kaki. Meanwhile, we also demonstrated that the MeGI gene was widespread in our investigated samples. Ultimately, our result convincingly provided evidence that the low expression of OGI is probably ascribed to the presence of Kali displaying strong methylation in the OGI promoter, and low expression of MeGI, as well as high DNA methylation level, in the promoter was closely connected with the production of male flowers; this result was consistent with the monoecious persimmon model. Our findings provide predominant genetic aspects for investigation into androecious D. kaki, and future perfecting the sex-determining mechanisms in persimmon. MDPI 2021-02-18 /pmc/articles/PMC7922513/ /pubmed/33670570 http://dx.doi.org/10.3390/plants10020390 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Liyuan
Han, Weijuan
Diao, Songfeng
Suo, Yujing
Li, Huawei
Mai, Yini
Wang, Yiru
Sun, Peng
Fu, Jianmin
Study of Sexual-Linked Genes (OGI and MeGI) on the Performance of Androecious Persimmons (Diospyros kaki Thunb.)
title Study of Sexual-Linked Genes (OGI and MeGI) on the Performance of Androecious Persimmons (Diospyros kaki Thunb.)
title_full Study of Sexual-Linked Genes (OGI and MeGI) on the Performance of Androecious Persimmons (Diospyros kaki Thunb.)
title_fullStr Study of Sexual-Linked Genes (OGI and MeGI) on the Performance of Androecious Persimmons (Diospyros kaki Thunb.)
title_full_unstemmed Study of Sexual-Linked Genes (OGI and MeGI) on the Performance of Androecious Persimmons (Diospyros kaki Thunb.)
title_short Study of Sexual-Linked Genes (OGI and MeGI) on the Performance of Androecious Persimmons (Diospyros kaki Thunb.)
title_sort study of sexual-linked genes (ogi and megi) on the performance of androecious persimmons (diospyros kaki thunb.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922513/
https://www.ncbi.nlm.nih.gov/pubmed/33670570
http://dx.doi.org/10.3390/plants10020390
work_keys_str_mv AT wangliyuan studyofsexuallinkedgenesogiandmegiontheperformanceofandroeciouspersimmonsdiospyroskakithunb
AT hanweijuan studyofsexuallinkedgenesogiandmegiontheperformanceofandroeciouspersimmonsdiospyroskakithunb
AT diaosongfeng studyofsexuallinkedgenesogiandmegiontheperformanceofandroeciouspersimmonsdiospyroskakithunb
AT suoyujing studyofsexuallinkedgenesogiandmegiontheperformanceofandroeciouspersimmonsdiospyroskakithunb
AT lihuawei studyofsexuallinkedgenesogiandmegiontheperformanceofandroeciouspersimmonsdiospyroskakithunb
AT maiyini studyofsexuallinkedgenesogiandmegiontheperformanceofandroeciouspersimmonsdiospyroskakithunb
AT wangyiru studyofsexuallinkedgenesogiandmegiontheperformanceofandroeciouspersimmonsdiospyroskakithunb
AT sunpeng studyofsexuallinkedgenesogiandmegiontheperformanceofandroeciouspersimmonsdiospyroskakithunb
AT fujianmin studyofsexuallinkedgenesogiandmegiontheperformanceofandroeciouspersimmonsdiospyroskakithunb