Cargando…

Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from Tetraclinis articulata (Vahl) Mast. Growing in Tunisia

The aim of the present research was to determine the chemical composition and the cytotoxic effects of Tetraclinis articulata trunk bark essential oil (HEE) obtained by steam distillation and five fractions obtained by normal phase silica chromatographic separation. Chemical analysis allowed the ide...

Descripción completa

Detalles Bibliográficos
Autores principales: Jlizi, Salma, Lahmar, Aida, Zardi-Bergaoui, Afifa, Ascrizzi, Roberta, Flamini, Guido, Harrath, Abdel Halim, Chekir-Ghedira, Leila, Ben Jannet, Hichem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922514/
https://www.ncbi.nlm.nih.gov/pubmed/33669825
http://dx.doi.org/10.3390/molecules26041110
_version_ 1783658707665551360
author Jlizi, Salma
Lahmar, Aida
Zardi-Bergaoui, Afifa
Ascrizzi, Roberta
Flamini, Guido
Harrath, Abdel Halim
Chekir-Ghedira, Leila
Ben Jannet, Hichem
author_facet Jlizi, Salma
Lahmar, Aida
Zardi-Bergaoui, Afifa
Ascrizzi, Roberta
Flamini, Guido
Harrath, Abdel Halim
Chekir-Ghedira, Leila
Ben Jannet, Hichem
author_sort Jlizi, Salma
collection PubMed
description The aim of the present research was to determine the chemical composition and the cytotoxic effects of Tetraclinis articulata trunk bark essential oil (HEE) obtained by steam distillation and five fractions obtained by normal phase silica chromatographic separation. Chemical analysis allowed the identification of 54 known compounds. Relatively high amounts of oxygenated sesquiterpenes (44.4–70.2%) were detected, mainly consisting of caryophyllene oxide (13.1–26.6%), carotol (9.2–21.2%),14-hydroxy-9-epi-(E)-caryophyllene (3.2–15.5%) and humulene epoxide II (2.6–7.2%). The cytotoxic activity against human mammary carcinoma cell lines (MDA-MB-231) and colorectal carcinoma cell lines (SW620) of the essential oil and its fractions were assessed. All the samples displayed moderate to weak activity compared to 5-fluorouracil. The colorectal carcinoma cell line was relatively more sensitive to the essential oil and its fractions compared to the breast cancer cell line, showing IC(50) values from 25.7 to 96.5 μg/mL. In addition, the essential oil and its fraction E.2 revealed a cytotoxic activity against colorectal carcinoma cell line, with IC(50) values lower than 30 μg/mL. This is the first report on the chemical composition and cytotoxic activity of the trunk bark essential oil of T. articulata.
format Online
Article
Text
id pubmed-7922514
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79225142021-03-03 Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from Tetraclinis articulata (Vahl) Mast. Growing in Tunisia Jlizi, Salma Lahmar, Aida Zardi-Bergaoui, Afifa Ascrizzi, Roberta Flamini, Guido Harrath, Abdel Halim Chekir-Ghedira, Leila Ben Jannet, Hichem Molecules Article The aim of the present research was to determine the chemical composition and the cytotoxic effects of Tetraclinis articulata trunk bark essential oil (HEE) obtained by steam distillation and five fractions obtained by normal phase silica chromatographic separation. Chemical analysis allowed the identification of 54 known compounds. Relatively high amounts of oxygenated sesquiterpenes (44.4–70.2%) were detected, mainly consisting of caryophyllene oxide (13.1–26.6%), carotol (9.2–21.2%),14-hydroxy-9-epi-(E)-caryophyllene (3.2–15.5%) and humulene epoxide II (2.6–7.2%). The cytotoxic activity against human mammary carcinoma cell lines (MDA-MB-231) and colorectal carcinoma cell lines (SW620) of the essential oil and its fractions were assessed. All the samples displayed moderate to weak activity compared to 5-fluorouracil. The colorectal carcinoma cell line was relatively more sensitive to the essential oil and its fractions compared to the breast cancer cell line, showing IC(50) values from 25.7 to 96.5 μg/mL. In addition, the essential oil and its fraction E.2 revealed a cytotoxic activity against colorectal carcinoma cell line, with IC(50) values lower than 30 μg/mL. This is the first report on the chemical composition and cytotoxic activity of the trunk bark essential oil of T. articulata. MDPI 2021-02-19 /pmc/articles/PMC7922514/ /pubmed/33669825 http://dx.doi.org/10.3390/molecules26041110 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jlizi, Salma
Lahmar, Aida
Zardi-Bergaoui, Afifa
Ascrizzi, Roberta
Flamini, Guido
Harrath, Abdel Halim
Chekir-Ghedira, Leila
Ben Jannet, Hichem
Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from Tetraclinis articulata (Vahl) Mast. Growing in Tunisia
title Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from Tetraclinis articulata (Vahl) Mast. Growing in Tunisia
title_full Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from Tetraclinis articulata (Vahl) Mast. Growing in Tunisia
title_fullStr Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from Tetraclinis articulata (Vahl) Mast. Growing in Tunisia
title_full_unstemmed Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from Tetraclinis articulata (Vahl) Mast. Growing in Tunisia
title_short Chemical Composition and Cytotoxic Activity of the Fractionated Trunk Bark Essential Oil from Tetraclinis articulata (Vahl) Mast. Growing in Tunisia
title_sort chemical composition and cytotoxic activity of the fractionated trunk bark essential oil from tetraclinis articulata (vahl) mast. growing in tunisia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922514/
https://www.ncbi.nlm.nih.gov/pubmed/33669825
http://dx.doi.org/10.3390/molecules26041110
work_keys_str_mv AT jlizisalma chemicalcompositionandcytotoxicactivityofthefractionatedtrunkbarkessentialoilfromtetraclinisarticulatavahlmastgrowingintunisia
AT lahmaraida chemicalcompositionandcytotoxicactivityofthefractionatedtrunkbarkessentialoilfromtetraclinisarticulatavahlmastgrowingintunisia
AT zardibergaouiafifa chemicalcompositionandcytotoxicactivityofthefractionatedtrunkbarkessentialoilfromtetraclinisarticulatavahlmastgrowingintunisia
AT ascrizziroberta chemicalcompositionandcytotoxicactivityofthefractionatedtrunkbarkessentialoilfromtetraclinisarticulatavahlmastgrowingintunisia
AT flaminiguido chemicalcompositionandcytotoxicactivityofthefractionatedtrunkbarkessentialoilfromtetraclinisarticulatavahlmastgrowingintunisia
AT harrathabdelhalim chemicalcompositionandcytotoxicactivityofthefractionatedtrunkbarkessentialoilfromtetraclinisarticulatavahlmastgrowingintunisia
AT chekirghediraleila chemicalcompositionandcytotoxicactivityofthefractionatedtrunkbarkessentialoilfromtetraclinisarticulatavahlmastgrowingintunisia
AT benjannethichem chemicalcompositionandcytotoxicactivityofthefractionatedtrunkbarkessentialoilfromtetraclinisarticulatavahlmastgrowingintunisia