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Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells

Background: Eupatilin is an active flavon extracted from the Artemisia species and has properties such as antioxidant, anti-inflammatory, and anti-cancer. We examined the effect of eupatilin using fine particulate matter (FPM) and human bronchial epithelial cell line (BEAS-2B) to confirm the potenti...

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Autores principales: Lee, Dong Chang, Oh, Jeong-Min, Choi, Hyunsu, Kim, Sung Won, Kim, Soo Whan, Kim, Byung Guk, Cho, Jin Hee, Lee, Joohyung, Kim, Ji-Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922545/
https://www.ncbi.nlm.nih.gov/pubmed/33670750
http://dx.doi.org/10.3390/toxics9020038
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author Lee, Dong Chang
Oh, Jeong-Min
Choi, Hyunsu
Kim, Sung Won
Kim, Soo Whan
Kim, Byung Guk
Cho, Jin Hee
Lee, Joohyung
Kim, Ji-Sun
author_facet Lee, Dong Chang
Oh, Jeong-Min
Choi, Hyunsu
Kim, Sung Won
Kim, Soo Whan
Kim, Byung Guk
Cho, Jin Hee
Lee, Joohyung
Kim, Ji-Sun
author_sort Lee, Dong Chang
collection PubMed
description Background: Eupatilin is an active flavon extracted from the Artemisia species and has properties such as antioxidant, anti-inflammatory, and anti-cancer. We examined the effect of eupatilin using fine particulate matter (FPM) and human bronchial epithelial cell line (BEAS-2B) to confirm the potential of eupatilin as a therapeutic agent for respiratory diseases caused by FPM. Methods: Reactive oxygen species (ROS) levels were checked by flow cytometry to identify if FPM and eupatilin affect ROS production. Western blotting was performed to identify the mechanism of action of eupatilin in FPM-exposed BEAS-2B cells. Results: When cells were exposed to FPM above 12.5 μg/mL concentration for 24 h, ROS production increased significantly compared to the control. When eupatilin was added to cells exposed to FPM, the ROS level decreased proportionally with the eupatilin dose. The phosphorylation of Akt, NF-κB p65, and p38 MAPK induced by FPM was significantly reduced by eupatilin, respectively. Conclusion: FPM cause respiratory disease by producing ROS in bronchial epithelial cells. Eupatilin has been shown to inhibit ROS production through altering signaling pathways. The ROS inhibiting property of eupatilin can be exploited in FPM induced respiratory disorders.
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spelling pubmed-79225452021-03-03 Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells Lee, Dong Chang Oh, Jeong-Min Choi, Hyunsu Kim, Sung Won Kim, Soo Whan Kim, Byung Guk Cho, Jin Hee Lee, Joohyung Kim, Ji-Sun Toxics Article Background: Eupatilin is an active flavon extracted from the Artemisia species and has properties such as antioxidant, anti-inflammatory, and anti-cancer. We examined the effect of eupatilin using fine particulate matter (FPM) and human bronchial epithelial cell line (BEAS-2B) to confirm the potential of eupatilin as a therapeutic agent for respiratory diseases caused by FPM. Methods: Reactive oxygen species (ROS) levels were checked by flow cytometry to identify if FPM and eupatilin affect ROS production. Western blotting was performed to identify the mechanism of action of eupatilin in FPM-exposed BEAS-2B cells. Results: When cells were exposed to FPM above 12.5 μg/mL concentration for 24 h, ROS production increased significantly compared to the control. When eupatilin was added to cells exposed to FPM, the ROS level decreased proportionally with the eupatilin dose. The phosphorylation of Akt, NF-κB p65, and p38 MAPK induced by FPM was significantly reduced by eupatilin, respectively. Conclusion: FPM cause respiratory disease by producing ROS in bronchial epithelial cells. Eupatilin has been shown to inhibit ROS production through altering signaling pathways. The ROS inhibiting property of eupatilin can be exploited in FPM induced respiratory disorders. MDPI 2021-02-18 /pmc/articles/PMC7922545/ /pubmed/33670750 http://dx.doi.org/10.3390/toxics9020038 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Dong Chang
Oh, Jeong-Min
Choi, Hyunsu
Kim, Sung Won
Kim, Soo Whan
Kim, Byung Guk
Cho, Jin Hee
Lee, Joohyung
Kim, Ji-Sun
Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells
title Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells
title_full Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells
title_fullStr Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells
title_full_unstemmed Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells
title_short Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells
title_sort eupatilin inhibits reactive oxygen species generation via akt/nf-κb/mapk signaling pathways in particulate matter-exposed human bronchial epithelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922545/
https://www.ncbi.nlm.nih.gov/pubmed/33670750
http://dx.doi.org/10.3390/toxics9020038
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