Cargando…
Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study
Diffuse large B-cell lymphoma (DLBCL) is the most common high-grade B-cell lymphoma found in Korea; it manifests with a variety of cellular morphologies and a high proliferation index. It is difficult to differentiate between DLBCL and Burkitt lymphoma (BL) based on immunohistochemistry, histology,...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922648/ https://www.ncbi.nlm.nih.gov/pubmed/33669569 http://dx.doi.org/10.3390/diagnostics11020343 |
_version_ | 1783658738297602048 |
---|---|
author | Chong, Yosep Kim, Tae Eun Cho, Uiju Jin, Min-Sun Yim, Kwangil Thakur, Nishant Kim, Jong Ok Cho, Inju Park, Gyeongsin |
author_facet | Chong, Yosep Kim, Tae Eun Cho, Uiju Jin, Min-Sun Yim, Kwangil Thakur, Nishant Kim, Jong Ok Cho, Inju Park, Gyeongsin |
author_sort | Chong, Yosep |
collection | PubMed |
description | Diffuse large B-cell lymphoma (DLBCL) is the most common high-grade B-cell lymphoma found in Korea; it manifests with a variety of cellular morphologies and a high proliferation index. It is difficult to differentiate between DLBCL and Burkitt lymphoma (BL) based on immunohistochemistry, histology, and Epstein-Barr virus infection status owing to the overlap in findings. In this study, we performed comparative morphometric analysis to understand the proportional difference in Ki-67 staining between DLBCL and BL. We analyzed Ki-67-stained slides of 103 DLBCLs and 29 BLs that were pathologically confirmed using a three-tier classification system (negative, 1+, 2+, and 3+) to compare Ki-67 expression between BL and activated B-cell and germinal center B-cell subtypes of DLBCL and DLBCL with high proliferation indices (>90% of 2+ and 3+ cells). Patients with DLBCL were older than those with BL (62.1 versus 51.0 years). The number and proportion of negative cells (passenger and true negative cells) were significantly lower in BLs than those in DLBCLs (337.4, 5.9% versus 690.3, 12.4%). The number and proportion of 3+ cells were significantly higher in BLs than those in DLBCLs (5213.6, 96.3% versus 3132.4, 62.0%). BLs and DLBCLs with a high proliferation index showed similar results as those between BLs and overall DLBCLs. We were able to differentiate BLs and DLBCLs with 98.1% sensitivity and 100.0% specificity using an optimal cut-off of 97.9% of 2+/3+ Ki-67-positive cells. Thus, the Ki-67 labeling index may be a good differential biomarker for DLBCLs and BLs. |
format | Online Article Text |
id | pubmed-7922648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79226482021-03-03 Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study Chong, Yosep Kim, Tae Eun Cho, Uiju Jin, Min-Sun Yim, Kwangil Thakur, Nishant Kim, Jong Ok Cho, Inju Park, Gyeongsin Diagnostics (Basel) Article Diffuse large B-cell lymphoma (DLBCL) is the most common high-grade B-cell lymphoma found in Korea; it manifests with a variety of cellular morphologies and a high proliferation index. It is difficult to differentiate between DLBCL and Burkitt lymphoma (BL) based on immunohistochemistry, histology, and Epstein-Barr virus infection status owing to the overlap in findings. In this study, we performed comparative morphometric analysis to understand the proportional difference in Ki-67 staining between DLBCL and BL. We analyzed Ki-67-stained slides of 103 DLBCLs and 29 BLs that were pathologically confirmed using a three-tier classification system (negative, 1+, 2+, and 3+) to compare Ki-67 expression between BL and activated B-cell and germinal center B-cell subtypes of DLBCL and DLBCL with high proliferation indices (>90% of 2+ and 3+ cells). Patients with DLBCL were older than those with BL (62.1 versus 51.0 years). The number and proportion of negative cells (passenger and true negative cells) were significantly lower in BLs than those in DLBCLs (337.4, 5.9% versus 690.3, 12.4%). The number and proportion of 3+ cells were significantly higher in BLs than those in DLBCLs (5213.6, 96.3% versus 3132.4, 62.0%). BLs and DLBCLs with a high proliferation index showed similar results as those between BLs and overall DLBCLs. We were able to differentiate BLs and DLBCLs with 98.1% sensitivity and 100.0% specificity using an optimal cut-off of 97.9% of 2+/3+ Ki-67-positive cells. Thus, the Ki-67 labeling index may be a good differential biomarker for DLBCLs and BLs. MDPI 2021-02-19 /pmc/articles/PMC7922648/ /pubmed/33669569 http://dx.doi.org/10.3390/diagnostics11020343 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chong, Yosep Kim, Tae Eun Cho, Uiju Jin, Min-Sun Yim, Kwangil Thakur, Nishant Kim, Jong Ok Cho, Inju Park, Gyeongsin Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study |
title | Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study |
title_full | Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study |
title_fullStr | Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study |
title_full_unstemmed | Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study |
title_short | Comparison of Ki-67 Labeling Index Patterns of Diffuse Large B-Cell Lymphomas and Burkitt Lymphomas Using Image Analysis: A Multicenter Study |
title_sort | comparison of ki-67 labeling index patterns of diffuse large b-cell lymphomas and burkitt lymphomas using image analysis: a multicenter study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922648/ https://www.ncbi.nlm.nih.gov/pubmed/33669569 http://dx.doi.org/10.3390/diagnostics11020343 |
work_keys_str_mv | AT chongyosep comparisonofki67labelingindexpatternsofdiffuselargebcelllymphomasandburkittlymphomasusingimageanalysisamulticenterstudy AT kimtaeeun comparisonofki67labelingindexpatternsofdiffuselargebcelllymphomasandburkittlymphomasusingimageanalysisamulticenterstudy AT chouiju comparisonofki67labelingindexpatternsofdiffuselargebcelllymphomasandburkittlymphomasusingimageanalysisamulticenterstudy AT jinminsun comparisonofki67labelingindexpatternsofdiffuselargebcelllymphomasandburkittlymphomasusingimageanalysisamulticenterstudy AT yimkwangil comparisonofki67labelingindexpatternsofdiffuselargebcelllymphomasandburkittlymphomasusingimageanalysisamulticenterstudy AT thakurnishant comparisonofki67labelingindexpatternsofdiffuselargebcelllymphomasandburkittlymphomasusingimageanalysisamulticenterstudy AT kimjongok comparisonofki67labelingindexpatternsofdiffuselargebcelllymphomasandburkittlymphomasusingimageanalysisamulticenterstudy AT choinju comparisonofki67labelingindexpatternsofdiffuselargebcelllymphomasandburkittlymphomasusingimageanalysisamulticenterstudy AT parkgyeongsin comparisonofki67labelingindexpatternsofdiffuselargebcelllymphomasandburkittlymphomasusingimageanalysisamulticenterstudy |