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Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis

Phage therapy is one of main alternative option for antibiotic treatment of bacterial infections, particularly in the era of appearance of pathogenic strains revealing resistance to most or even all known antibiotics. Enterococcus faecalis is one of such pathogens causing serious human infections. I...

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Autores principales: Topka-Bielecka, Gracja, Nejman-Faleńczyk, Bożena, Bloch, Sylwia, Dydecka, Aleksandra, Necel, Agnieszka, Węgrzyn, Alicja, Węgrzyn, Grzegorz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922982/
https://www.ncbi.nlm.nih.gov/pubmed/33669643
http://dx.doi.org/10.3390/v13020318
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author Topka-Bielecka, Gracja
Nejman-Faleńczyk, Bożena
Bloch, Sylwia
Dydecka, Aleksandra
Necel, Agnieszka
Węgrzyn, Alicja
Węgrzyn, Grzegorz
author_facet Topka-Bielecka, Gracja
Nejman-Faleńczyk, Bożena
Bloch, Sylwia
Dydecka, Aleksandra
Necel, Agnieszka
Węgrzyn, Alicja
Węgrzyn, Grzegorz
author_sort Topka-Bielecka, Gracja
collection PubMed
description Phage therapy is one of main alternative option for antibiotic treatment of bacterial infections, particularly in the era of appearance of pathogenic strains revealing resistance to most or even all known antibiotics. Enterococcus faecalis is one of such pathogens causing serious human infections. In the light of high level of biodiversity of bacteriophages and specificity of phages to bacterial species or even strains, development of effective phage therapy depend, between others, on identification and characterization of a large collection of these viruses, including understanding of their interactions with host bacterial cells. Recently, isolation of molecular characterization of bacteriophage vB_EfaS-271, infecting E. faecalis strains have been reported. In this report, phage–host interactions are reported, including ability of vB_EfaS-271 to infect bacteria forming biofilms, efficiency of eliminating bacterial cells from cultures depending on multiplicity of infection (m.o.i.), toxicity of purified phage particles to mammalian cells, and efficiency of appearance of phage-resistant bacteria. The presented results indicate that vB_EfaS-271 can significantly decrease number of viable E. faecalis cells in biofilms and in liquid cultures and reveals no considerable toxicity to mammalian cells. Efficiency of formation of phage-resistant bacteria was dependent on m.o.i. and was higher when the virion-cell ratio was as high as 10 than at low (between 0.01 and 0.0001) m.o.i. values. We conclude that vB_EfaS-271 may be considered as a candidate for its further use in phage therapy.
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spelling pubmed-79229822021-03-03 Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis Topka-Bielecka, Gracja Nejman-Faleńczyk, Bożena Bloch, Sylwia Dydecka, Aleksandra Necel, Agnieszka Węgrzyn, Alicja Węgrzyn, Grzegorz Viruses Article Phage therapy is one of main alternative option for antibiotic treatment of bacterial infections, particularly in the era of appearance of pathogenic strains revealing resistance to most or even all known antibiotics. Enterococcus faecalis is one of such pathogens causing serious human infections. In the light of high level of biodiversity of bacteriophages and specificity of phages to bacterial species or even strains, development of effective phage therapy depend, between others, on identification and characterization of a large collection of these viruses, including understanding of their interactions with host bacterial cells. Recently, isolation of molecular characterization of bacteriophage vB_EfaS-271, infecting E. faecalis strains have been reported. In this report, phage–host interactions are reported, including ability of vB_EfaS-271 to infect bacteria forming biofilms, efficiency of eliminating bacterial cells from cultures depending on multiplicity of infection (m.o.i.), toxicity of purified phage particles to mammalian cells, and efficiency of appearance of phage-resistant bacteria. The presented results indicate that vB_EfaS-271 can significantly decrease number of viable E. faecalis cells in biofilms and in liquid cultures and reveals no considerable toxicity to mammalian cells. Efficiency of formation of phage-resistant bacteria was dependent on m.o.i. and was higher when the virion-cell ratio was as high as 10 than at low (between 0.01 and 0.0001) m.o.i. values. We conclude that vB_EfaS-271 may be considered as a candidate for its further use in phage therapy. MDPI 2021-02-19 /pmc/articles/PMC7922982/ /pubmed/33669643 http://dx.doi.org/10.3390/v13020318 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Topka-Bielecka, Gracja
Nejman-Faleńczyk, Bożena
Bloch, Sylwia
Dydecka, Aleksandra
Necel, Agnieszka
Węgrzyn, Alicja
Węgrzyn, Grzegorz
Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis
title Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis
title_full Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis
title_fullStr Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis
title_full_unstemmed Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis
title_short Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis
title_sort phage–bacteria interactions in potential applications of bacteriophage vb_efas-271 against enterococcus faecalis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922982/
https://www.ncbi.nlm.nih.gov/pubmed/33669643
http://dx.doi.org/10.3390/v13020318
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