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Oleuropein alleviates gestational diabetes mellitus by activating AMPK signaling

BACKGROUND: Gestational diabetes mellitus (GDM) has a high incidence rate among pregnant women. The objective of the study was to assess the effect of plant-derived oleuropein in attenuating inflammatory and oxidative stress of GDM. METHODS: Oleuropein was administered to GDM mice at the doses of 5...

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Autores principales: Zhang, Zhiwei, Zhao, Hui, Wang, Aixia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscientifica Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923051/
https://www.ncbi.nlm.nih.gov/pubmed/33289688
http://dx.doi.org/10.1530/EC-20-0466
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author Zhang, Zhiwei
Zhao, Hui
Wang, Aixia
author_facet Zhang, Zhiwei
Zhao, Hui
Wang, Aixia
author_sort Zhang, Zhiwei
collection PubMed
description BACKGROUND: Gestational diabetes mellitus (GDM) has a high incidence rate among pregnant women. The objective of the study was to assess the effect of plant-derived oleuropein in attenuating inflammatory and oxidative stress of GDM. METHODS: Oleuropein was administered to GDM mice at the doses of 5 or 10 mg/kg/day. Body weight, blood glucose, insulin and hepatic glycogen levels were recorded. To evaluate the effect of oleuropein in reducing oxidative stress, ELISA was used to measure the hepatic oxidative stress markers. The inflammation levels of GDM mice were evaluated by measuring serum levels of IL-6 and TNF-α by ELISA and mRNA levels of IL-1β, TNF-α and IL-6 by real-time PCR (RT-PCR). The AMP-activated protein kinase (AMPK) signaling pathway was assessed by Western blot. Gestational outcome was analyzed through comparing litter size and birth weight. RESULTS: Oleuropein attenuated the elevated body weight of GDM mice and efficiently reduced blood glucose, insulin and hepatic glycogen levels. Oxidative stress and inflammation were alleviated by oleuropein treatment. The AMPK signaling was activated by oleuropein in GDM mice. Gestational outcome was markedly improved by oleuropein treatment. CONCLUSIONS: Our study suggests that oleuropein is effective in alleviating symptoms of GDM and improving gestational outcome in the mouse model. This effect is achieved by attenuating oxidative stress and inflammation, which is mediated by the activation of the AMPK signaling pathway.
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spelling pubmed-79230512021-03-05 Oleuropein alleviates gestational diabetes mellitus by activating AMPK signaling Zhang, Zhiwei Zhao, Hui Wang, Aixia Endocr Connect Research BACKGROUND: Gestational diabetes mellitus (GDM) has a high incidence rate among pregnant women. The objective of the study was to assess the effect of plant-derived oleuropein in attenuating inflammatory and oxidative stress of GDM. METHODS: Oleuropein was administered to GDM mice at the doses of 5 or 10 mg/kg/day. Body weight, blood glucose, insulin and hepatic glycogen levels were recorded. To evaluate the effect of oleuropein in reducing oxidative stress, ELISA was used to measure the hepatic oxidative stress markers. The inflammation levels of GDM mice were evaluated by measuring serum levels of IL-6 and TNF-α by ELISA and mRNA levels of IL-1β, TNF-α and IL-6 by real-time PCR (RT-PCR). The AMP-activated protein kinase (AMPK) signaling pathway was assessed by Western blot. Gestational outcome was analyzed through comparing litter size and birth weight. RESULTS: Oleuropein attenuated the elevated body weight of GDM mice and efficiently reduced blood glucose, insulin and hepatic glycogen levels. Oxidative stress and inflammation were alleviated by oleuropein treatment. The AMPK signaling was activated by oleuropein in GDM mice. Gestational outcome was markedly improved by oleuropein treatment. CONCLUSIONS: Our study suggests that oleuropein is effective in alleviating symptoms of GDM and improving gestational outcome in the mouse model. This effect is achieved by attenuating oxidative stress and inflammation, which is mediated by the activation of the AMPK signaling pathway. Bioscientifica Ltd 2020-11-30 /pmc/articles/PMC7923051/ /pubmed/33289688 http://dx.doi.org/10.1530/EC-20-0466 Text en © 2021 The authors http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. (http://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle Research
Zhang, Zhiwei
Zhao, Hui
Wang, Aixia
Oleuropein alleviates gestational diabetes mellitus by activating AMPK signaling
title Oleuropein alleviates gestational diabetes mellitus by activating AMPK signaling
title_full Oleuropein alleviates gestational diabetes mellitus by activating AMPK signaling
title_fullStr Oleuropein alleviates gestational diabetes mellitus by activating AMPK signaling
title_full_unstemmed Oleuropein alleviates gestational diabetes mellitus by activating AMPK signaling
title_short Oleuropein alleviates gestational diabetes mellitus by activating AMPK signaling
title_sort oleuropein alleviates gestational diabetes mellitus by activating ampk signaling
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923051/
https://www.ncbi.nlm.nih.gov/pubmed/33289688
http://dx.doi.org/10.1530/EC-20-0466
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