Cargando…
The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica
Biological nematicides have been widely used to lower the losses generated by phytoparasitic nematodes. The purpose of this study was to evaluate the nematicidal effects of Escherichia coli BL21(DE3) against Meloidogyne javanica and to identify nematicide-related genes. Culture filtrates of BL21(DE3...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923116/ https://www.ncbi.nlm.nih.gov/pubmed/33670696 http://dx.doi.org/10.3390/pathogens10020222 |
_version_ | 1783658840439390208 |
---|---|
author | Xia, Yanfei Li, Shen Xu, Guohui Xie, Shanshan Liu, Xueting Lin, Xiaomin Wu, Huijun Gao, Xuewen |
author_facet | Xia, Yanfei Li, Shen Xu, Guohui Xie, Shanshan Liu, Xueting Lin, Xiaomin Wu, Huijun Gao, Xuewen |
author_sort | Xia, Yanfei |
collection | PubMed |
description | Biological nematicides have been widely used to lower the losses generated by phytoparasitic nematodes. The purpose of this study was to evaluate the nematicidal effects of Escherichia coli BL21(DE3) against Meloidogyne javanica and to identify nematicide-related genes. Culture filtrates of BL21(DE3) caused juvenile mortality and inhibited egg hatching in a dose-dependent manner. In the greenhouse, treatment of tomato seedlings with BL21(DE3) culture filtrates at 50 and 100% concentrations not only reduced the amount of M. javanica egg masses and galls, but improved plant root and shoot fresh weight. Culture filtrate analysis indicated that the nematicidal active ingredients of strain BL21(DE3) were non-proteinaceous, heat and cold resistant, sensitive to pH and volatile. To identify the genes associated with nematicidal activity, a BL21(DE3) library of 5000 mutants was produced using Tn5 transposase insertion. The culture filtrate of the MB12 mutant showed no nematicidal activity after 72 h of treatment and thermal asymmetrical interlaced PCR demonstrated that the carB gene was disrupted. Nematicidal activity was restored when the pH of the MB12 culture filtrate was adjusted to the original pH value (4.15) or following MB12 complementation with the carB gene, confirming a role for carB in mediating pH value and nematicidal activity. The outcomes of this pilot study indicate that BL21(DE3) is a potential microorganism for the continuable biological control of root-knot nematode in tomato and that carB affects the nematicidal activity of BL21(DE3) by modulating the pH environment. |
format | Online Article Text |
id | pubmed-7923116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79231162021-03-03 The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica Xia, Yanfei Li, Shen Xu, Guohui Xie, Shanshan Liu, Xueting Lin, Xiaomin Wu, Huijun Gao, Xuewen Pathogens Article Biological nematicides have been widely used to lower the losses generated by phytoparasitic nematodes. The purpose of this study was to evaluate the nematicidal effects of Escherichia coli BL21(DE3) against Meloidogyne javanica and to identify nematicide-related genes. Culture filtrates of BL21(DE3) caused juvenile mortality and inhibited egg hatching in a dose-dependent manner. In the greenhouse, treatment of tomato seedlings with BL21(DE3) culture filtrates at 50 and 100% concentrations not only reduced the amount of M. javanica egg masses and galls, but improved plant root and shoot fresh weight. Culture filtrate analysis indicated that the nematicidal active ingredients of strain BL21(DE3) were non-proteinaceous, heat and cold resistant, sensitive to pH and volatile. To identify the genes associated with nematicidal activity, a BL21(DE3) library of 5000 mutants was produced using Tn5 transposase insertion. The culture filtrate of the MB12 mutant showed no nematicidal activity after 72 h of treatment and thermal asymmetrical interlaced PCR demonstrated that the carB gene was disrupted. Nematicidal activity was restored when the pH of the MB12 culture filtrate was adjusted to the original pH value (4.15) or following MB12 complementation with the carB gene, confirming a role for carB in mediating pH value and nematicidal activity. The outcomes of this pilot study indicate that BL21(DE3) is a potential microorganism for the continuable biological control of root-knot nematode in tomato and that carB affects the nematicidal activity of BL21(DE3) by modulating the pH environment. MDPI 2021-02-18 /pmc/articles/PMC7923116/ /pubmed/33670696 http://dx.doi.org/10.3390/pathogens10020222 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xia, Yanfei Li, Shen Xu, Guohui Xie, Shanshan Liu, Xueting Lin, Xiaomin Wu, Huijun Gao, Xuewen The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica |
title | The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica |
title_full | The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica |
title_fullStr | The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica |
title_full_unstemmed | The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica |
title_short | The carB Gene of Escherichia coli BL21(DE3) is Associated with Nematicidal Activity against the Root-Knot Nematode Meloidogyne javanica |
title_sort | carb gene of escherichia coli bl21(de3) is associated with nematicidal activity against the root-knot nematode meloidogyne javanica |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923116/ https://www.ncbi.nlm.nih.gov/pubmed/33670696 http://dx.doi.org/10.3390/pathogens10020222 |
work_keys_str_mv | AT xiayanfei thecarbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT lishen thecarbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT xuguohui thecarbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT xieshanshan thecarbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT liuxueting thecarbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT linxiaomin thecarbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT wuhuijun thecarbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT gaoxuewen thecarbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT xiayanfei carbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT lishen carbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT xuguohui carbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT xieshanshan carbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT liuxueting carbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT linxiaomin carbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT wuhuijun carbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica AT gaoxuewen carbgeneofescherichiacolibl21de3isassociatedwithnematicidalactivityagainsttherootknotnematodemeloidogynejavanica |