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Expression of Lymphatic Markers in the Berger’s Space and Bursa Premacularis

We previously reported that the bursa premacularis (BPM), a peculiar vitreous structure located above the macula, contains numerous cells expressing markers of lymphatic endothelial cells, such as podoplanin and LYVE-1. Herein, we examined the expression of lymphatic markers in the Berger’s space (B...

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Autores principales: Morishita, Seita, Sato, Takaki, Oosuka, Shou, Horie, Taeko, Kida, Teruyo, Oku, Hidehiro, Nakamura, Kimitoshi, Takai, Shinji, Jin, Denan, Ikeda, Tsunehiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923221/
https://www.ncbi.nlm.nih.gov/pubmed/33669860
http://dx.doi.org/10.3390/ijms22042086
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author Morishita, Seita
Sato, Takaki
Oosuka, Shou
Horie, Taeko
Kida, Teruyo
Oku, Hidehiro
Nakamura, Kimitoshi
Takai, Shinji
Jin, Denan
Ikeda, Tsunehiko
author_facet Morishita, Seita
Sato, Takaki
Oosuka, Shou
Horie, Taeko
Kida, Teruyo
Oku, Hidehiro
Nakamura, Kimitoshi
Takai, Shinji
Jin, Denan
Ikeda, Tsunehiko
author_sort Morishita, Seita
collection PubMed
description We previously reported that the bursa premacularis (BPM), a peculiar vitreous structure located above the macula, contains numerous cells expressing markers of lymphatic endothelial cells, such as podoplanin and LYVE-1. Herein, we examined the expression of lymphatic markers in the Berger’s space (BS), BPM, and vitreous core (VC). BS, BPM, and VC specimens were selectively collected in macular hole and epiretinal membrane patients during vitrectomy and were then immunostained with antibodies for podoplanin, LYVE-1, and fibrillin-1 and -2. By visualization using triamcinolone acetonide, the BS was recognized as a sac-like structure with a septum located behind the lens as well as BPM. Those tissues adhered to the lens or retina in a circular manner by means of a ligament-like structure. Immunostaining showed intense expression of podoplanin and LYVE-1 in the BS. Both BS and BPM stained strongly positive for fibrillin-1 and -2. The VC was faintly stained with antibodies for those lymph-node markers. Our findings indicate that both BS and BPM possibly belong to the lymphatic system, such as lymph nodes, draining excess fluid and waste products into lymphatic vessels in the dura mater of the optic nerve and the ciliary body, respectively, via intravitreal canals.
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spelling pubmed-79232212021-03-03 Expression of Lymphatic Markers in the Berger’s Space and Bursa Premacularis Morishita, Seita Sato, Takaki Oosuka, Shou Horie, Taeko Kida, Teruyo Oku, Hidehiro Nakamura, Kimitoshi Takai, Shinji Jin, Denan Ikeda, Tsunehiko Int J Mol Sci Article We previously reported that the bursa premacularis (BPM), a peculiar vitreous structure located above the macula, contains numerous cells expressing markers of lymphatic endothelial cells, such as podoplanin and LYVE-1. Herein, we examined the expression of lymphatic markers in the Berger’s space (BS), BPM, and vitreous core (VC). BS, BPM, and VC specimens were selectively collected in macular hole and epiretinal membrane patients during vitrectomy and were then immunostained with antibodies for podoplanin, LYVE-1, and fibrillin-1 and -2. By visualization using triamcinolone acetonide, the BS was recognized as a sac-like structure with a septum located behind the lens as well as BPM. Those tissues adhered to the lens or retina in a circular manner by means of a ligament-like structure. Immunostaining showed intense expression of podoplanin and LYVE-1 in the BS. Both BS and BPM stained strongly positive for fibrillin-1 and -2. The VC was faintly stained with antibodies for those lymph-node markers. Our findings indicate that both BS and BPM possibly belong to the lymphatic system, such as lymph nodes, draining excess fluid and waste products into lymphatic vessels in the dura mater of the optic nerve and the ciliary body, respectively, via intravitreal canals. MDPI 2021-02-19 /pmc/articles/PMC7923221/ /pubmed/33669860 http://dx.doi.org/10.3390/ijms22042086 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Morishita, Seita
Sato, Takaki
Oosuka, Shou
Horie, Taeko
Kida, Teruyo
Oku, Hidehiro
Nakamura, Kimitoshi
Takai, Shinji
Jin, Denan
Ikeda, Tsunehiko
Expression of Lymphatic Markers in the Berger’s Space and Bursa Premacularis
title Expression of Lymphatic Markers in the Berger’s Space and Bursa Premacularis
title_full Expression of Lymphatic Markers in the Berger’s Space and Bursa Premacularis
title_fullStr Expression of Lymphatic Markers in the Berger’s Space and Bursa Premacularis
title_full_unstemmed Expression of Lymphatic Markers in the Berger’s Space and Bursa Premacularis
title_short Expression of Lymphatic Markers in the Berger’s Space and Bursa Premacularis
title_sort expression of lymphatic markers in the berger’s space and bursa premacularis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923221/
https://www.ncbi.nlm.nih.gov/pubmed/33669860
http://dx.doi.org/10.3390/ijms22042086
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