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New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes
Changes that occur within oviducts after fertilization are dependent on post-ovulation events, including oocyte-oviduct interactions. Although general processes are well-defined, the molecular basis are poorly understood. Recently, new marker genes involved in ‘cell development’, ‘cell growth’, ‘cel...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923230/ https://www.ncbi.nlm.nih.gov/pubmed/33669854 http://dx.doi.org/10.3390/ijms22042082 |
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author | Kulus, Magdalena Kranc, Wiesława Wojtanowicz-Markiewicz, Katarzyna Celichowski, Piotr Światły-Błaszkiewicz, Agata Matuszewska, Eliza Sujka-Kordowska, Patrycja Konwerska, Aneta Zdun, Maciej Bryl, Rut Wieczorkiewicz, Maria Kulus, Jakub Stelmach, Bogusława Stefańska, Katarzyna Budna-Tukan, Joanna Petitte, James N. Mozdziak, Paul Ratajczak, Kornel Matysiak, Jan Jaśkowski, Jędrzej M. Nowicki, Michał Kempisty, Bartosz |
author_facet | Kulus, Magdalena Kranc, Wiesława Wojtanowicz-Markiewicz, Katarzyna Celichowski, Piotr Światły-Błaszkiewicz, Agata Matuszewska, Eliza Sujka-Kordowska, Patrycja Konwerska, Aneta Zdun, Maciej Bryl, Rut Wieczorkiewicz, Maria Kulus, Jakub Stelmach, Bogusława Stefańska, Katarzyna Budna-Tukan, Joanna Petitte, James N. Mozdziak, Paul Ratajczak, Kornel Matysiak, Jan Jaśkowski, Jędrzej M. Nowicki, Michał Kempisty, Bartosz |
author_sort | Kulus, Magdalena |
collection | PubMed |
description | Changes that occur within oviducts after fertilization are dependent on post-ovulation events, including oocyte-oviduct interactions. Although general processes are well-defined, the molecular basis are poorly understood. Recently, new marker genes involved in ‘cell development’, ‘cell growth’, ‘cell differentiation’ and ‘cell maturation’ processes have been identified in porcine oocytes. The aim of the study was to assess the expression profile of genes in primary in vitro cultured oviductal epithelial cells (OECs), clustered in Gene Ontology groups which enveloped markers also identified in porcine oocytes. OECs (from 45 gilts) were surgically removed and cultured in vitro for ≤ 30 days, and then subjected to molecular analyses. The transcriptomic and proteomic profiles of cells cultured during 7, 15 and 30 days were investigated. Additionally, morphological/histochemical analyzes were performed. The results of genes expression profiles were validated after using RT-qPCR. The results showed a significant upregulation of UNC45B, NOX4, VLDLR, ITGB3, FMOD, SGCE, COL1A2, LOX, LIPG, THY1 and downregulation of SERPINB2, CD274, TXNIP, CELA1, DDX60, CRABP2, SLC5A1, IDO1, ANPEP, FST. Detailed knowledge of the molecular pathways occurring in the OECs and the gametes that contact them may contribute both to developments of basic science of physiology, and new possibilities in advanced biotechnology of assisted reproduction. |
format | Online Article Text |
id | pubmed-7923230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79232302021-03-03 New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes Kulus, Magdalena Kranc, Wiesława Wojtanowicz-Markiewicz, Katarzyna Celichowski, Piotr Światły-Błaszkiewicz, Agata Matuszewska, Eliza Sujka-Kordowska, Patrycja Konwerska, Aneta Zdun, Maciej Bryl, Rut Wieczorkiewicz, Maria Kulus, Jakub Stelmach, Bogusława Stefańska, Katarzyna Budna-Tukan, Joanna Petitte, James N. Mozdziak, Paul Ratajczak, Kornel Matysiak, Jan Jaśkowski, Jędrzej M. Nowicki, Michał Kempisty, Bartosz Int J Mol Sci Article Changes that occur within oviducts after fertilization are dependent on post-ovulation events, including oocyte-oviduct interactions. Although general processes are well-defined, the molecular basis are poorly understood. Recently, new marker genes involved in ‘cell development’, ‘cell growth’, ‘cell differentiation’ and ‘cell maturation’ processes have been identified in porcine oocytes. The aim of the study was to assess the expression profile of genes in primary in vitro cultured oviductal epithelial cells (OECs), clustered in Gene Ontology groups which enveloped markers also identified in porcine oocytes. OECs (from 45 gilts) were surgically removed and cultured in vitro for ≤ 30 days, and then subjected to molecular analyses. The transcriptomic and proteomic profiles of cells cultured during 7, 15 and 30 days were investigated. Additionally, morphological/histochemical analyzes were performed. The results of genes expression profiles were validated after using RT-qPCR. The results showed a significant upregulation of UNC45B, NOX4, VLDLR, ITGB3, FMOD, SGCE, COL1A2, LOX, LIPG, THY1 and downregulation of SERPINB2, CD274, TXNIP, CELA1, DDX60, CRABP2, SLC5A1, IDO1, ANPEP, FST. Detailed knowledge of the molecular pathways occurring in the OECs and the gametes that contact them may contribute both to developments of basic science of physiology, and new possibilities in advanced biotechnology of assisted reproduction. MDPI 2021-02-19 /pmc/articles/PMC7923230/ /pubmed/33669854 http://dx.doi.org/10.3390/ijms22042082 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kulus, Magdalena Kranc, Wiesława Wojtanowicz-Markiewicz, Katarzyna Celichowski, Piotr Światły-Błaszkiewicz, Agata Matuszewska, Eliza Sujka-Kordowska, Patrycja Konwerska, Aneta Zdun, Maciej Bryl, Rut Wieczorkiewicz, Maria Kulus, Jakub Stelmach, Bogusława Stefańska, Katarzyna Budna-Tukan, Joanna Petitte, James N. Mozdziak, Paul Ratajczak, Kornel Matysiak, Jan Jaśkowski, Jędrzej M. Nowicki, Michał Kempisty, Bartosz New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes |
title | New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes |
title_full | New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes |
title_fullStr | New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes |
title_full_unstemmed | New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes |
title_short | New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes |
title_sort | new gene markers expressed in porcine oviductal epithelial cells cultured primary in vitro are involved in ontological groups representing physiological processes of porcine oocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923230/ https://www.ncbi.nlm.nih.gov/pubmed/33669854 http://dx.doi.org/10.3390/ijms22042082 |
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