Cargando…
Novel methylated DNA markers accurately discriminate Lynch syndrome associated colorectal neoplasia
AIM: Acquired molecular changes in Lynch syndrome (LS) colorectal tumors have been largely unstudied. We identified methylated DNA markers (MDMs) for discrimination of colorectal neoplasia in LS and determined if these MDMs were comparably discriminant in sporadic patients. PATIENTS & METHODS: F...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Medicine Ltd
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923255/ https://www.ncbi.nlm.nih.gov/pubmed/33350853 http://dx.doi.org/10.2217/epi-2020-0132 |
_version_ | 1783658870501015552 |
---|---|
author | Ballester, Veroushka Taylor, William R Slettedahl, Seth W Mahoney, Douglas W Yab, Tracy C Sinicrope, Frank A Boland, Clement R Lidgard, Graham P Cruz-Correa, Marcia R Smyrk, Thomas C Boardman, Lisa A Ahlquist, David A Kisiel, John B |
author_facet | Ballester, Veroushka Taylor, William R Slettedahl, Seth W Mahoney, Douglas W Yab, Tracy C Sinicrope, Frank A Boland, Clement R Lidgard, Graham P Cruz-Correa, Marcia R Smyrk, Thomas C Boardman, Lisa A Ahlquist, David A Kisiel, John B |
author_sort | Ballester, Veroushka |
collection | PubMed |
description | AIM: Acquired molecular changes in Lynch syndrome (LS) colorectal tumors have been largely unstudied. We identified methylated DNA markers (MDMs) for discrimination of colorectal neoplasia in LS and determined if these MDMs were comparably discriminant in sporadic patients. PATIENTS & METHODS: For LS discovery, we evaluated DNA from 53 colorectal case and control tissues using next generation sequencing. For validation, blinded methylation-specific PCR assays to the selected MDMs were performed on 197 cases and controls. RESULTS: OPLAH was the most discriminant MDM with areas under the receiver operating characteristic curve ≥0.97 for colorectal neoplasia in LS and sporadic tissues. ALKBH5, was uniquely hypermethylated in LS neoplasms. CONCLUSION: Highly discriminant MDMs for colorectal neoplasia in LS were identified with potential use in screening and surveillance. |
format | Online Article Text |
id | pubmed-7923255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Future Medicine Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-79232552021-03-03 Novel methylated DNA markers accurately discriminate Lynch syndrome associated colorectal neoplasia Ballester, Veroushka Taylor, William R Slettedahl, Seth W Mahoney, Douglas W Yab, Tracy C Sinicrope, Frank A Boland, Clement R Lidgard, Graham P Cruz-Correa, Marcia R Smyrk, Thomas C Boardman, Lisa A Ahlquist, David A Kisiel, John B Epigenomics Research Article AIM: Acquired molecular changes in Lynch syndrome (LS) colorectal tumors have been largely unstudied. We identified methylated DNA markers (MDMs) for discrimination of colorectal neoplasia in LS and determined if these MDMs were comparably discriminant in sporadic patients. PATIENTS & METHODS: For LS discovery, we evaluated DNA from 53 colorectal case and control tissues using next generation sequencing. For validation, blinded methylation-specific PCR assays to the selected MDMs were performed on 197 cases and controls. RESULTS: OPLAH was the most discriminant MDM with areas under the receiver operating characteristic curve ≥0.97 for colorectal neoplasia in LS and sporadic tissues. ALKBH5, was uniquely hypermethylated in LS neoplasms. CONCLUSION: Highly discriminant MDMs for colorectal neoplasia in LS were identified with potential use in screening and surveillance. Future Medicine Ltd 2020-12-22 2020-12 /pmc/articles/PMC7923255/ /pubmed/33350853 http://dx.doi.org/10.2217/epi-2020-0132 Text en © 2020 John B Kisiel This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Research Article Ballester, Veroushka Taylor, William R Slettedahl, Seth W Mahoney, Douglas W Yab, Tracy C Sinicrope, Frank A Boland, Clement R Lidgard, Graham P Cruz-Correa, Marcia R Smyrk, Thomas C Boardman, Lisa A Ahlquist, David A Kisiel, John B Novel methylated DNA markers accurately discriminate Lynch syndrome associated colorectal neoplasia |
title | Novel methylated DNA markers accurately discriminate Lynch syndrome associated colorectal neoplasia |
title_full | Novel methylated DNA markers accurately discriminate Lynch syndrome associated colorectal neoplasia |
title_fullStr | Novel methylated DNA markers accurately discriminate Lynch syndrome associated colorectal neoplasia |
title_full_unstemmed | Novel methylated DNA markers accurately discriminate Lynch syndrome associated colorectal neoplasia |
title_short | Novel methylated DNA markers accurately discriminate Lynch syndrome associated colorectal neoplasia |
title_sort | novel methylated dna markers accurately discriminate lynch syndrome associated colorectal neoplasia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923255/ https://www.ncbi.nlm.nih.gov/pubmed/33350853 http://dx.doi.org/10.2217/epi-2020-0132 |
work_keys_str_mv | AT ballesterveroushka novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT taylorwilliamr novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT slettedahlsethw novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT mahoneydouglasw novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT yabtracyc novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT sinicropefranka novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT bolandclementr novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT lidgardgrahamp novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT cruzcorreamarciar novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT smyrkthomasc novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT boardmanlisaa novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT ahlquistdavida novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia AT kisieljohnb novelmethylateddnamarkersaccuratelydiscriminatelynchsyndromeassociatedcolorectalneoplasia |