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Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber

Plant cystatins are cysteine proteinase inhibitors that play key roles in defense responses. In this work, we describe an unexpected role for the cystatin-like protein DEFORMED FLORAL BUD1 (CsDFB1) as a transcriptional regulator of local auxin distribution in cucumber (Cucumis sativus L.). CsDFB1 wa...

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Autores principales: Nie, Jing, Shan, Nan, Liu, Huan, Yao, Xuehui, Wang, Ziwei, Bai, Ruoxue, Guo, Yicong, Duan, Ying, Wang, Changlin, Sui, Xiaolei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923377/
https://www.ncbi.nlm.nih.gov/pubmed/33602821
http://dx.doi.org/10.1073/pnas.2023942118
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author Nie, Jing
Shan, Nan
Liu, Huan
Yao, Xuehui
Wang, Ziwei
Bai, Ruoxue
Guo, Yicong
Duan, Ying
Wang, Changlin
Sui, Xiaolei
author_facet Nie, Jing
Shan, Nan
Liu, Huan
Yao, Xuehui
Wang, Ziwei
Bai, Ruoxue
Guo, Yicong
Duan, Ying
Wang, Changlin
Sui, Xiaolei
author_sort Nie, Jing
collection PubMed
description Plant cystatins are cysteine proteinase inhibitors that play key roles in defense responses. In this work, we describe an unexpected role for the cystatin-like protein DEFORMED FLORAL BUD1 (CsDFB1) as a transcriptional regulator of local auxin distribution in cucumber (Cucumis sativus L.). CsDFB1 was strongly expressed in the floral meristems, floral primordia, and vasculature. RNA interference (RNAi)-mediated silencing of CsDFB1 led to a significantly increased number of floral organs and vascular bundles, together with a pronounced accumulation of auxin. Conversely, accompanied by a decrease of auxin, overexpression of CsDFB1 resulted in a dramatic reduction in floral organ number and an obvious defect in vascular patterning, as well as organ fusion. CsDFB1 physically interacted with the cucumber ortholog of PHABULOSA (CsPHB), an HD-ZIP III transcription factor whose transcripts exhibit the same pattern as CsDFB1. Overexpression of CsPHB increased auxin accumulation in shoot tips and induced a floral phenotype similar to that of CsDFB1-RNAi lines. Furthermore, genetic and biochemical analyses revealed that CsDFB1 impairs CsPHB-mediated transcriptional regulation of the auxin biosynthetic gene YUCCA2 and the auxin efflux carrier PIN-FORMED1, and thus plays a pivotal role in auxin distribution. In summary, we propose that the CsDFB1-CsPHB module represents a regulatory pathway for local auxin distribution that governs floral organogenesis and vascular differentiation in cucumber.
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spelling pubmed-79233772021-03-10 Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber Nie, Jing Shan, Nan Liu, Huan Yao, Xuehui Wang, Ziwei Bai, Ruoxue Guo, Yicong Duan, Ying Wang, Changlin Sui, Xiaolei Proc Natl Acad Sci U S A Biological Sciences Plant cystatins are cysteine proteinase inhibitors that play key roles in defense responses. In this work, we describe an unexpected role for the cystatin-like protein DEFORMED FLORAL BUD1 (CsDFB1) as a transcriptional regulator of local auxin distribution in cucumber (Cucumis sativus L.). CsDFB1 was strongly expressed in the floral meristems, floral primordia, and vasculature. RNA interference (RNAi)-mediated silencing of CsDFB1 led to a significantly increased number of floral organs and vascular bundles, together with a pronounced accumulation of auxin. Conversely, accompanied by a decrease of auxin, overexpression of CsDFB1 resulted in a dramatic reduction in floral organ number and an obvious defect in vascular patterning, as well as organ fusion. CsDFB1 physically interacted with the cucumber ortholog of PHABULOSA (CsPHB), an HD-ZIP III transcription factor whose transcripts exhibit the same pattern as CsDFB1. Overexpression of CsPHB increased auxin accumulation in shoot tips and induced a floral phenotype similar to that of CsDFB1-RNAi lines. Furthermore, genetic and biochemical analyses revealed that CsDFB1 impairs CsPHB-mediated transcriptional regulation of the auxin biosynthetic gene YUCCA2 and the auxin efflux carrier PIN-FORMED1, and thus plays a pivotal role in auxin distribution. In summary, we propose that the CsDFB1-CsPHB module represents a regulatory pathway for local auxin distribution that governs floral organogenesis and vascular differentiation in cucumber. National Academy of Sciences 2021-02-23 2021-02-18 /pmc/articles/PMC7923377/ /pubmed/33602821 http://dx.doi.org/10.1073/pnas.2023942118 Text en Copyright © 2021 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Nie, Jing
Shan, Nan
Liu, Huan
Yao, Xuehui
Wang, Ziwei
Bai, Ruoxue
Guo, Yicong
Duan, Ying
Wang, Changlin
Sui, Xiaolei
Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber
title Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber
title_full Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber
title_fullStr Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber
title_full_unstemmed Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber
title_short Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber
title_sort transcriptional control of local auxin distribution by the csdfb1-csphb module regulates floral organogenesis in cucumber
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923377/
https://www.ncbi.nlm.nih.gov/pubmed/33602821
http://dx.doi.org/10.1073/pnas.2023942118
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