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Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences
Most eukaryotes possess two RecA-like recombinases (ubiquitous Rad51 and meiosis-specific Dmc1) to promote interhomolog recombination during meiosis. However, some eukaryotes have lost Dmc1. Given that mammalian and yeast Saccharomyces cerevisiae (Sc) Dmc1 have been shown to stabilize recombination...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923544/ https://www.ncbi.nlm.nih.gov/pubmed/33593897 http://dx.doi.org/10.1073/pnas.2007192118 |
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author | Li, Wan-Chen Lee, Chia-Yi Lan, Wei-Hsuan Woo, Tai-Ting Liu, Hou-Cheng Yeh, Hsin-Yi Chang, Hao-Yen Chuang, Yu-Chien Chen, Chiung-Ya Chuang, Chi-Ning Chen, Chia-Ling Hsueh, Yi-Ping Li, Hung-Wen Chi, Peter Wang, Ting-Fang |
author_facet | Li, Wan-Chen Lee, Chia-Yi Lan, Wei-Hsuan Woo, Tai-Ting Liu, Hou-Cheng Yeh, Hsin-Yi Chang, Hao-Yen Chuang, Yu-Chien Chen, Chiung-Ya Chuang, Chi-Ning Chen, Chia-Ling Hsueh, Yi-Ping Li, Hung-Wen Chi, Peter Wang, Ting-Fang |
author_sort | Li, Wan-Chen |
collection | PubMed |
description | Most eukaryotes possess two RecA-like recombinases (ubiquitous Rad51 and meiosis-specific Dmc1) to promote interhomolog recombination during meiosis. However, some eukaryotes have lost Dmc1. Given that mammalian and yeast Saccharomyces cerevisiae (Sc) Dmc1 have been shown to stabilize recombination intermediates containing mismatches better than Rad51, we used the Pezizomycotina filamentous fungus Trichoderma reesei to address if and how Rad51-only eukaryotes conduct interhomolog recombination in zygotes with high sequence heterogeneity. We applied multidisciplinary approaches (next- and third-generation sequencing technology, genetics, cytology, bioinformatics, biochemistry, and single-molecule biophysics) to show that T. reesei Rad51 (TrRad51) is indispensable for interhomolog recombination during meiosis and, like ScDmc1, TrRad51 possesses better mismatch tolerance than ScRad51 during homologous recombination. Our results also indicate that the ancestral TrRad51 evolved to acquire ScDmc1-like properties by creating multiple structural variations, including via amino acid residues in the L1 and L2 DNA-binding loops. |
format | Online Article Text |
id | pubmed-7923544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-79235442021-03-10 Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences Li, Wan-Chen Lee, Chia-Yi Lan, Wei-Hsuan Woo, Tai-Ting Liu, Hou-Cheng Yeh, Hsin-Yi Chang, Hao-Yen Chuang, Yu-Chien Chen, Chiung-Ya Chuang, Chi-Ning Chen, Chia-Ling Hsueh, Yi-Ping Li, Hung-Wen Chi, Peter Wang, Ting-Fang Proc Natl Acad Sci U S A Biological Sciences Most eukaryotes possess two RecA-like recombinases (ubiquitous Rad51 and meiosis-specific Dmc1) to promote interhomolog recombination during meiosis. However, some eukaryotes have lost Dmc1. Given that mammalian and yeast Saccharomyces cerevisiae (Sc) Dmc1 have been shown to stabilize recombination intermediates containing mismatches better than Rad51, we used the Pezizomycotina filamentous fungus Trichoderma reesei to address if and how Rad51-only eukaryotes conduct interhomolog recombination in zygotes with high sequence heterogeneity. We applied multidisciplinary approaches (next- and third-generation sequencing technology, genetics, cytology, bioinformatics, biochemistry, and single-molecule biophysics) to show that T. reesei Rad51 (TrRad51) is indispensable for interhomolog recombination during meiosis and, like ScDmc1, TrRad51 possesses better mismatch tolerance than ScRad51 during homologous recombination. Our results also indicate that the ancestral TrRad51 evolved to acquire ScDmc1-like properties by creating multiple structural variations, including via amino acid residues in the L1 and L2 DNA-binding loops. National Academy of Sciences 2021-02-23 2021-02-15 /pmc/articles/PMC7923544/ /pubmed/33593897 http://dx.doi.org/10.1073/pnas.2007192118 Text en Copyright © 2021 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Li, Wan-Chen Lee, Chia-Yi Lan, Wei-Hsuan Woo, Tai-Ting Liu, Hou-Cheng Yeh, Hsin-Yi Chang, Hao-Yen Chuang, Yu-Chien Chen, Chiung-Ya Chuang, Chi-Ning Chen, Chia-Ling Hsueh, Yi-Ping Li, Hung-Wen Chi, Peter Wang, Ting-Fang Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences |
title | Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences |
title_full | Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences |
title_fullStr | Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences |
title_full_unstemmed | Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences |
title_short | Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences |
title_sort | trichoderma reesei rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923544/ https://www.ncbi.nlm.nih.gov/pubmed/33593897 http://dx.doi.org/10.1073/pnas.2007192118 |
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