Cargando…
Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains
In this contribution, we studied the effect of electro‐fermentation on the butanol production of Clostridium pasteurianum strains by a targeted metabolomics approach. Two strains were examined: an electrocompetent wild type strain (R525) and a mutant strain (dhaB mutant) lacking formation of 1,3‐pro...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923553/ https://www.ncbi.nlm.nih.gov/pubmed/33716617 http://dx.doi.org/10.1002/elsc.202000035 |
_version_ | 1783658925646675968 |
---|---|
author | Arbter, Philipp Sabra, Wael Utesch, Tyll Hong, Yaeseong Zeng, An‐Ping |
author_facet | Arbter, Philipp Sabra, Wael Utesch, Tyll Hong, Yaeseong Zeng, An‐Ping |
author_sort | Arbter, Philipp |
collection | PubMed |
description | In this contribution, we studied the effect of electro‐fermentation on the butanol production of Clostridium pasteurianum strains by a targeted metabolomics approach. Two strains were examined: an electrocompetent wild type strain (R525) and a mutant strain (dhaB mutant) lacking formation of 1,3‐propanediol (PDO). The dhaB‐negative strain was able to grow on glycerol without formation of PDO, but displayed a high initial intracellular NADH/NAD ratio which was lowered subsequently by upregulation of the butanol production pathway. Both strains showed a 3–5 fold increase of the intracellular NADH/NAD ratio when exposed to cathodic current in a bioelectrochemical system (BES). This drove an activation of the butanol pathway and resulted in a higher molar butanol to PDO ratio for the R525 strain. Nonetheless, macroscopic electron balances suggest that no significant amount of electrons derived from the BES was harvested by the cells. Overall, this work points out that electro‐fermentation can be used to trigger metabolic pathways and improve product formation, even when the used microbe cannot be considered electroactive. Accordingly, further studies are required to unveil the underlying (regulatory) mechanisms. |
format | Online Article Text |
id | pubmed-7923553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79235532021-03-12 Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains Arbter, Philipp Sabra, Wael Utesch, Tyll Hong, Yaeseong Zeng, An‐Ping Eng Life Sci Research Articles In this contribution, we studied the effect of electro‐fermentation on the butanol production of Clostridium pasteurianum strains by a targeted metabolomics approach. Two strains were examined: an electrocompetent wild type strain (R525) and a mutant strain (dhaB mutant) lacking formation of 1,3‐propanediol (PDO). The dhaB‐negative strain was able to grow on glycerol without formation of PDO, but displayed a high initial intracellular NADH/NAD ratio which was lowered subsequently by upregulation of the butanol production pathway. Both strains showed a 3–5 fold increase of the intracellular NADH/NAD ratio when exposed to cathodic current in a bioelectrochemical system (BES). This drove an activation of the butanol pathway and resulted in a higher molar butanol to PDO ratio for the R525 strain. Nonetheless, macroscopic electron balances suggest that no significant amount of electrons derived from the BES was harvested by the cells. Overall, this work points out that electro‐fermentation can be used to trigger metabolic pathways and improve product formation, even when the used microbe cannot be considered electroactive. Accordingly, further studies are required to unveil the underlying (regulatory) mechanisms. John Wiley and Sons Inc. 2020-12-06 /pmc/articles/PMC7923553/ /pubmed/33716617 http://dx.doi.org/10.1002/elsc.202000035 Text en © 2020 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Arbter, Philipp Sabra, Wael Utesch, Tyll Hong, Yaeseong Zeng, An‐Ping Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains |
title | Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains |
title_full | Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains |
title_fullStr | Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains |
title_full_unstemmed | Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains |
title_short | Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains |
title_sort | metabolomic and kinetic investigations on the electricity‐aided production of butanol by clostridium pasteurianum strains |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923553/ https://www.ncbi.nlm.nih.gov/pubmed/33716617 http://dx.doi.org/10.1002/elsc.202000035 |
work_keys_str_mv | AT arbterphilipp metabolomicandkineticinvestigationsontheelectricityaidedproductionofbutanolbyclostridiumpasteurianumstrains AT sabrawael metabolomicandkineticinvestigationsontheelectricityaidedproductionofbutanolbyclostridiumpasteurianumstrains AT uteschtyll metabolomicandkineticinvestigationsontheelectricityaidedproductionofbutanolbyclostridiumpasteurianumstrains AT hongyaeseong metabolomicandkineticinvestigationsontheelectricityaidedproductionofbutanolbyclostridiumpasteurianumstrains AT zenganping metabolomicandkineticinvestigationsontheelectricityaidedproductionofbutanolbyclostridiumpasteurianumstrains |