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Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors
In this study, the biocatalytic performance of a Baeyer‐Villiger monooxygenase (BVMO) catalyzing the reaction of cyclohexanone to ε‐caprolactone was investigated in Pseudomonas biofilms. Biofilm growth and development of two Pseudomonas taiwanensis VLB120 variants, Ps_BVMO and Ps_BVMO_DGC, were eval...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923564/ https://www.ncbi.nlm.nih.gov/pubmed/33716623 http://dx.doi.org/10.1002/elsc.202000072 |
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author | Heuschkel, Ingeborg Hanisch, Selina Volke, Daniel C. Löfgren, Erik Hoschek, Anna Nikel, Pablo I. Karande, Rohan Bühler, Katja |
author_facet | Heuschkel, Ingeborg Hanisch, Selina Volke, Daniel C. Löfgren, Erik Hoschek, Anna Nikel, Pablo I. Karande, Rohan Bühler, Katja |
author_sort | Heuschkel, Ingeborg |
collection | PubMed |
description | In this study, the biocatalytic performance of a Baeyer‐Villiger monooxygenase (BVMO) catalyzing the reaction of cyclohexanone to ε‐caprolactone was investigated in Pseudomonas biofilms. Biofilm growth and development of two Pseudomonas taiwanensis VLB120 variants, Ps_BVMO and Ps_BVMO_DGC, were evaluated in drip flow reactors (DFRs) and rotating bed reactors (RBRs). Engineering a hyperactive diguanylate cyclase (DGC) from Caulobacter crescentus into Ps_BVMO resulted in faster biofilm growth compared to the control Ps_BVMO strain in the DFRs. The maximum product formation rates of 92 and 87 g m(–2) d(–1) were observed for mature Ps_BVMO and Ps_ BVMO_DGC biofilms, respectively. The application of the engineered variants in the RBR was challenged by low biofilm surface coverage (50–60%) of rotating bed cassettes, side‐products formation, oxygen limitation, and a severe drop in production rates with time. By implementing an active oxygen supply mode and a twin capillary spray feed, the biofilm surface coverage was maximized to 70–80%. BVMO activity was severely inhibited by cyclohexanol formation, resulting in a decrease in product formation rates. By controlling the cyclohexanone feed concentration at 4 mM, a stable product formation rate of 14 g m(–2) d(–1) and a substrate conversion of 60% was achieved in the RBR. |
format | Online Article Text |
id | pubmed-7923564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79235642021-03-12 Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors Heuschkel, Ingeborg Hanisch, Selina Volke, Daniel C. Löfgren, Erik Hoschek, Anna Nikel, Pablo I. Karande, Rohan Bühler, Katja Eng Life Sci Research Articles In this study, the biocatalytic performance of a Baeyer‐Villiger monooxygenase (BVMO) catalyzing the reaction of cyclohexanone to ε‐caprolactone was investigated in Pseudomonas biofilms. Biofilm growth and development of two Pseudomonas taiwanensis VLB120 variants, Ps_BVMO and Ps_BVMO_DGC, were evaluated in drip flow reactors (DFRs) and rotating bed reactors (RBRs). Engineering a hyperactive diguanylate cyclase (DGC) from Caulobacter crescentus into Ps_BVMO resulted in faster biofilm growth compared to the control Ps_BVMO strain in the DFRs. The maximum product formation rates of 92 and 87 g m(–2) d(–1) were observed for mature Ps_BVMO and Ps_ BVMO_DGC biofilms, respectively. The application of the engineered variants in the RBR was challenged by low biofilm surface coverage (50–60%) of rotating bed cassettes, side‐products formation, oxygen limitation, and a severe drop in production rates with time. By implementing an active oxygen supply mode and a twin capillary spray feed, the biofilm surface coverage was maximized to 70–80%. BVMO activity was severely inhibited by cyclohexanol formation, resulting in a decrease in product formation rates. By controlling the cyclohexanone feed concentration at 4 mM, a stable product formation rate of 14 g m(–2) d(–1) and a substrate conversion of 60% was achieved in the RBR. John Wiley and Sons Inc. 2021-02-02 /pmc/articles/PMC7923564/ /pubmed/33716623 http://dx.doi.org/10.1002/elsc.202000072 Text en © 2021 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Heuschkel, Ingeborg Hanisch, Selina Volke, Daniel C. Löfgren, Erik Hoschek, Anna Nikel, Pablo I. Karande, Rohan Bühler, Katja Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors |
title |
Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors |
title_full |
Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors |
title_fullStr |
Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors |
title_full_unstemmed |
Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors |
title_short |
Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors |
title_sort | pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923564/ https://www.ncbi.nlm.nih.gov/pubmed/33716623 http://dx.doi.org/10.1002/elsc.202000072 |
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