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Rheological and Microstructural Evaluation of Collagen-Based Scaffolds Crosslinked with Fructose

In recent years, tissue engineering research has led to the development of this field by designing scaffolds with better properties that can fulfill its purpose of better and faster tissue regeneration, consequently improving people’s quality of life. Scaffolds are matrices, predominantly composed o...

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Autores principales: Sánchez-Cid, Pablo, Jiménez‑Rosado, Mercedes, Perez-Puyana, Victor, Guerrero, Antonio, Romero, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923766/
https://www.ncbi.nlm.nih.gov/pubmed/33672532
http://dx.doi.org/10.3390/polym13040632
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author Sánchez-Cid, Pablo
Jiménez‑Rosado, Mercedes
Perez-Puyana, Victor
Guerrero, Antonio
Romero, Alberto
author_facet Sánchez-Cid, Pablo
Jiménez‑Rosado, Mercedes
Perez-Puyana, Victor
Guerrero, Antonio
Romero, Alberto
author_sort Sánchez-Cid, Pablo
collection PubMed
description In recent years, tissue engineering research has led to the development of this field by designing scaffolds with better properties that can fulfill its purpose of better and faster tissue regeneration, consequently improving people’s quality of life. Scaffolds are matrices, predominantly composed of polymeric materials, whose main function is to offer support for cell adhesion and subsequent growth, leading to the regeneration of the damaged tissue. The widely used biopolymer in tissue engineering is collagen, which is the most abundant protein in animals. Its use is due to its structure, biocompatibility, ease of modification, and processability. In this work, collagen-based scaffolds were developed with different concentrations and processing techniques, by obtaining hydrogels and aerogels that were characterized with an emphasis on their morphology and mechanical properties. Moreover, fructose was added in some cases as a chemical crosslinking agent to study its influence on the scaffolds’ properties. The obtained results revealed that the scaffolds with higher collagen concentrations were more rigid and deformable. Comparing both systems, the aerogels were more rigid, although the hydrogels were more deformable and had higher pore size homogeneity. Fructose addition produced a slight increase in the critical strain, together with an increase in the elastic modulus.
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spelling pubmed-79237662021-03-03 Rheological and Microstructural Evaluation of Collagen-Based Scaffolds Crosslinked with Fructose Sánchez-Cid, Pablo Jiménez‑Rosado, Mercedes Perez-Puyana, Victor Guerrero, Antonio Romero, Alberto Polymers (Basel) Article In recent years, tissue engineering research has led to the development of this field by designing scaffolds with better properties that can fulfill its purpose of better and faster tissue regeneration, consequently improving people’s quality of life. Scaffolds are matrices, predominantly composed of polymeric materials, whose main function is to offer support for cell adhesion and subsequent growth, leading to the regeneration of the damaged tissue. The widely used biopolymer in tissue engineering is collagen, which is the most abundant protein in animals. Its use is due to its structure, biocompatibility, ease of modification, and processability. In this work, collagen-based scaffolds were developed with different concentrations and processing techniques, by obtaining hydrogels and aerogels that were characterized with an emphasis on their morphology and mechanical properties. Moreover, fructose was added in some cases as a chemical crosslinking agent to study its influence on the scaffolds’ properties. The obtained results revealed that the scaffolds with higher collagen concentrations were more rigid and deformable. Comparing both systems, the aerogels were more rigid, although the hydrogels were more deformable and had higher pore size homogeneity. Fructose addition produced a slight increase in the critical strain, together with an increase in the elastic modulus. MDPI 2021-02-20 /pmc/articles/PMC7923766/ /pubmed/33672532 http://dx.doi.org/10.3390/polym13040632 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sánchez-Cid, Pablo
Jiménez‑Rosado, Mercedes
Perez-Puyana, Victor
Guerrero, Antonio
Romero, Alberto
Rheological and Microstructural Evaluation of Collagen-Based Scaffolds Crosslinked with Fructose
title Rheological and Microstructural Evaluation of Collagen-Based Scaffolds Crosslinked with Fructose
title_full Rheological and Microstructural Evaluation of Collagen-Based Scaffolds Crosslinked with Fructose
title_fullStr Rheological and Microstructural Evaluation of Collagen-Based Scaffolds Crosslinked with Fructose
title_full_unstemmed Rheological and Microstructural Evaluation of Collagen-Based Scaffolds Crosslinked with Fructose
title_short Rheological and Microstructural Evaluation of Collagen-Based Scaffolds Crosslinked with Fructose
title_sort rheological and microstructural evaluation of collagen-based scaffolds crosslinked with fructose
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923766/
https://www.ncbi.nlm.nih.gov/pubmed/33672532
http://dx.doi.org/10.3390/polym13040632
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