Effect of a Phytogenic Water Additive on Growth Performance, Blood Metabolites and Gene Expression of Amino Acid Transporters in Nursery Pigs Fed with Low-Protein/High-Carbohydrate Diets

SIMPLE SUMMARY: Low-protein (LP) diets can be potentially used to reduce the excretion of nitrogenous compounds and feed cost in commercial swine production; however, new strategies are required to be developed to improve the growth performance of pigs receiving these diets. Little is known about the effect of phytogenic additives on the performance of pigs fed with LP diets and the underlying factors involved. The objective of this study was to assess the effect of a phytogenic water additive (PWA) on growth performance, nutrient digestibility, blood metabolites, plasma amino acid (AA) concentration, and gut and skeletal muscle AA transporters in nursery pigs fed with LP diets. Supplemental PWA increased the concentration of circulating essential AA, reduced the transcript of some of the AA transporters in the small intestine and skeletal muscle, improved growth performance when the dietary protein was adequate, and increased muscle lean%, but reduced muscle fat% when the dietary protein was deficient. The used PWA in this study had differential effects on blood calcium and its digestibility depending on the level of dietary protein. This study suggests that PWA can be used for improving the meat composition in protein-restricted pigs, but PWA improves growth performance only when dietary protein is adequate. ABSTRACT: The objective of this study was to investigate the effect of a phytogenic water additive (PWA) on growth performance and underlying factors involved in pigs fed with low-protein (LP)/high-carbohydrate diets. Forty-eight weaned barrows were allotted to six treatments for 4 weeks: CON-NS, control (CON) diet-no PWA; CON-LS, CON diet-low dose PWA (4 mL/L); CON-HS, CON diet-high dose PWA (8 mL/L); LP-NS, LP diet-no PWA; LP-LS, LP diet-low dose PWA; LP-HS, LP diet-high dose PWA. Relative to CON-NS, pigs fed with CON-HS had increased average daily gain, body weight and serum calcium (Ca) and phosphorous (P) and had decreased mRNA abundance of solute carrier family 7 member 11 and solute carrier family 6 member 19 in jejunum. Compared to LP-NS, pigs fed with LP-HS had increased muscle lean%, decreased muscle fat%, decreased serum Ca and increased serum P. Compared to their NS counterparts, CON-LS, CON-HS, and LP-LS increased the concentration of plasma essential AA and those fed with CON-HS and LP-HS tended to reduce the abundance of the solute carrier family 7 member 1 transcript in skeletal muscle. Thus, PWA improved the performance of weaned pigs fed with protein-adequate diets likely through increased blood essential AA and affected the muscle composition when dietary protein was deficient.