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The Effectiveness of Dichloroacetate on Human Glioblastoma Xenograft Growth Depends on Na+ and Mg2+ Cations

The study’s aim was to investigate the effectiveness of sodium dichloroacetate (NaDCA) or magnesium dichloroacetate (MgDCA) on adult U87 MG and pediatric PBT24 cell lines glioblastoma (GB) xenografts in a chicken chorioallantoic membrane (CAM) model. The study groups were: treated with 10 mM, 5 mM o...

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Autores principales: Stakišaitis, Donatas, Damanskienė, Eligija, Curkūnavičiūtė, Rūta, Juknevičienė, Milda, Alonso, Marta Maria, Valančiūtė, Angelija, Ročka, Saulius, Balnytė, Ingrida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923996/
https://www.ncbi.nlm.nih.gov/pubmed/33716589
http://dx.doi.org/10.1177/1559325821990166
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author Stakišaitis, Donatas
Damanskienė, Eligija
Curkūnavičiūtė, Rūta
Juknevičienė, Milda
Alonso, Marta Maria
Valančiūtė, Angelija
Ročka, Saulius
Balnytė, Ingrida
author_facet Stakišaitis, Donatas
Damanskienė, Eligija
Curkūnavičiūtė, Rūta
Juknevičienė, Milda
Alonso, Marta Maria
Valančiūtė, Angelija
Ročka, Saulius
Balnytė, Ingrida
author_sort Stakišaitis, Donatas
collection PubMed
description The study’s aim was to investigate the effectiveness of sodium dichloroacetate (NaDCA) or magnesium dichloroacetate (MgDCA) on adult U87 MG and pediatric PBT24 cell lines glioblastoma (GB) xenografts in a chicken chorioallantoic membrane (CAM) model. The study groups were: treated with 10 mM, 5 mM of NaDCA, and 5 mM, 2.5 mM of MgDCA, and controls. The U87 MG and PBT24 xenografts growth, frequency of tumor invasion into CAM, CAM thickening, and the number of blood vessels in CAM differed depending on the dichloroacetate salt treatment. NaDCA impact on U87 MG and PBT24 tumor on proliferating cell nunclear antigen (PCNA) and enhancer of zeste homolog 2 (EZH2) expression in the tumor was different, depending on the NaDCA dose. The 5 mM MgDCA impact was more potent and had similar effects on U87 MG and PBT24 tumors, and its impact was also reflected in changes in PCNA and EZH2 expression in tumor cells. The U87 MG and PBT24 tumor response variations to treatment with different NaDCA concentration on tumor growth or a contrast between NaDCA and MgDCA effectiveness may reflect some differences in U87 MG and PBT24 cell biology.
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spelling pubmed-79239962021-03-11 The Effectiveness of Dichloroacetate on Human Glioblastoma Xenograft Growth Depends on Na+ and Mg2+ Cations Stakišaitis, Donatas Damanskienė, Eligija Curkūnavičiūtė, Rūta Juknevičienė, Milda Alonso, Marta Maria Valančiūtė, Angelija Ročka, Saulius Balnytė, Ingrida Dose Response Original Article The study’s aim was to investigate the effectiveness of sodium dichloroacetate (NaDCA) or magnesium dichloroacetate (MgDCA) on adult U87 MG and pediatric PBT24 cell lines glioblastoma (GB) xenografts in a chicken chorioallantoic membrane (CAM) model. The study groups were: treated with 10 mM, 5 mM of NaDCA, and 5 mM, 2.5 mM of MgDCA, and controls. The U87 MG and PBT24 xenografts growth, frequency of tumor invasion into CAM, CAM thickening, and the number of blood vessels in CAM differed depending on the dichloroacetate salt treatment. NaDCA impact on U87 MG and PBT24 tumor on proliferating cell nunclear antigen (PCNA) and enhancer of zeste homolog 2 (EZH2) expression in the tumor was different, depending on the NaDCA dose. The 5 mM MgDCA impact was more potent and had similar effects on U87 MG and PBT24 tumors, and its impact was also reflected in changes in PCNA and EZH2 expression in tumor cells. The U87 MG and PBT24 tumor response variations to treatment with different NaDCA concentration on tumor growth or a contrast between NaDCA and MgDCA effectiveness may reflect some differences in U87 MG and PBT24 cell biology. SAGE Publications 2021-02-27 /pmc/articles/PMC7923996/ /pubmed/33716589 http://dx.doi.org/10.1177/1559325821990166 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Stakišaitis, Donatas
Damanskienė, Eligija
Curkūnavičiūtė, Rūta
Juknevičienė, Milda
Alonso, Marta Maria
Valančiūtė, Angelija
Ročka, Saulius
Balnytė, Ingrida
The Effectiveness of Dichloroacetate on Human Glioblastoma Xenograft Growth Depends on Na+ and Mg2+ Cations
title The Effectiveness of Dichloroacetate on Human Glioblastoma Xenograft Growth Depends on Na+ and Mg2+ Cations
title_full The Effectiveness of Dichloroacetate on Human Glioblastoma Xenograft Growth Depends on Na+ and Mg2+ Cations
title_fullStr The Effectiveness of Dichloroacetate on Human Glioblastoma Xenograft Growth Depends on Na+ and Mg2+ Cations
title_full_unstemmed The Effectiveness of Dichloroacetate on Human Glioblastoma Xenograft Growth Depends on Na+ and Mg2+ Cations
title_short The Effectiveness of Dichloroacetate on Human Glioblastoma Xenograft Growth Depends on Na+ and Mg2+ Cations
title_sort effectiveness of dichloroacetate on human glioblastoma xenograft growth depends on na+ and mg2+ cations
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7923996/
https://www.ncbi.nlm.nih.gov/pubmed/33716589
http://dx.doi.org/10.1177/1559325821990166
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