Cargando…
A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2
The SARS-CoV-2 pandemic has caused over 1 million deaths globally, mostly due to acute lung injury and acute respiratory distress syndrome, or direct complications resulting in multiple-organ failures. Little is known about the host tissue immune and cellular responses associated with COVID-19 infec...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924267/ https://www.ncbi.nlm.nih.gov/pubmed/33655247 http://dx.doi.org/10.1101/2021.02.25.430130 |
_version_ | 1783659054846967808 |
---|---|
author | Delorey, Toni M. Ziegler, Carly G. K. Heimberg, Graham Normand, Rachelly Yang, Yiming Segerstolpe, Asa Abbondanza, Domenic Fleming, Stephen J. Subramanian, Ayshwarya Montoro, Daniel T. Jagadeesh, Karthik A. Dey, Kushal K. Sen, Pritha Slyper, Michal Pita-Juárez, Yered H. Phillips, Devan Bloom-Ackerman, Zohar Barkas, Nick Ganna, Andrea Gomez, James Normandin, Erica Naderi, Pourya Popov, Yury V. Raju, Siddharth S. Niezen, Sebastian Tsai, Linus T.-Y. Siddle, Katherine J. Sud, Malika Tran, Victoria M. Vellarikkal, Shamsudheen K. Amir-Zilberstein, Liat Atri, Deepak S. Beechem, Joseph Brook, Olga R. Chen, Jonathan Divakar, Prajan Dorceus, Phylicia Engreitz, Jesse M. Essene, Adam Fitzgerald, Donna M. Fropf, Robin Gazal, Steven Gould, Joshua Grzyb, John Harvey, Tyler Hecht, Jonathan Hether, Tyler Jane-Valbuena, Judit Leney-Greene, Michael Ma, Hui McCabe, Cristin McLoughlin, Daniel E. Miller, Eric M. Muus, Christoph Niemi, Mari Padera, Robert Pan, Liuliu Pant, Deepti Pe’er, Carmel Pfiffner-Borges, Jenna Pinto, Christopher J. Plaisted, Jacob Reeves, Jason Ross, Marty Rudy, Melissa Rueckert, Erroll H. Siciliano, Michelle Sturm, Alexander Todres, Ellen Waghray, Avinash Warren, Sarah Zhang, Shuting Zollinger, Daniel R. Cosimi, Lisa Gupta, Rajat M. Hacohen, Nir Hide, Winston Price, Alkes L. Rajagopal, Jayaraj Tata, Purushothama Rao Riedel, Stefan Szabo, Gyongyi Tickle, Timothy L. Hung, Deborah Sabeti, Pardis C. Novak, Richard Rogers, Robert Ingber, Donald E. Jiang, Z. Gordon Juric, Dejan Babadi, Mehrtash Farhi, Samouil L. Stone, James R. Vlachos, Ioannis S. Solomon, Isaac H. Ashenberg, Orr Porter, Caroline B.M. Li, Bo Shalek, Alex K. Villani, Alexandra-Chloé Rozenblatt-Rosen, Orit Regev, Aviv |
author_facet | Delorey, Toni M. Ziegler, Carly G. K. Heimberg, Graham Normand, Rachelly Yang, Yiming Segerstolpe, Asa Abbondanza, Domenic Fleming, Stephen J. Subramanian, Ayshwarya Montoro, Daniel T. Jagadeesh, Karthik A. Dey, Kushal K. Sen, Pritha Slyper, Michal Pita-Juárez, Yered H. Phillips, Devan Bloom-Ackerman, Zohar Barkas, Nick Ganna, Andrea Gomez, James Normandin, Erica Naderi, Pourya Popov, Yury V. Raju, Siddharth S. Niezen, Sebastian Tsai, Linus T.-Y. Siddle, Katherine J. Sud, Malika Tran, Victoria M. Vellarikkal, Shamsudheen K. Amir-Zilberstein, Liat Atri, Deepak S. Beechem, Joseph Brook, Olga R. Chen, Jonathan Divakar, Prajan Dorceus, Phylicia Engreitz, Jesse M. Essene, Adam Fitzgerald, Donna M. Fropf, Robin Gazal, Steven Gould, Joshua Grzyb, John Harvey, Tyler Hecht, Jonathan Hether, Tyler Jane-Valbuena, Judit Leney-Greene, Michael Ma, Hui McCabe, Cristin McLoughlin, Daniel E. Miller, Eric M. Muus, Christoph Niemi, Mari Padera, Robert Pan, Liuliu Pant, Deepti Pe’er, Carmel Pfiffner-Borges, Jenna Pinto, Christopher J. Plaisted, Jacob Reeves, Jason Ross, Marty Rudy, Melissa Rueckert, Erroll H. Siciliano, Michelle Sturm, Alexander Todres, Ellen Waghray, Avinash Warren, Sarah Zhang, Shuting Zollinger, Daniel R. Cosimi, Lisa Gupta, Rajat M. Hacohen, Nir Hide, Winston Price, Alkes L. Rajagopal, Jayaraj Tata, Purushothama Rao Riedel, Stefan Szabo, Gyongyi Tickle, Timothy L. Hung, Deborah Sabeti, Pardis C. Novak, Richard Rogers, Robert Ingber, Donald E. Jiang, Z. Gordon Juric, Dejan Babadi, Mehrtash Farhi, Samouil L. Stone, James R. Vlachos, Ioannis S. Solomon, Isaac H. Ashenberg, Orr Porter, Caroline B.M. Li, Bo Shalek, Alex K. Villani, Alexandra-Chloé Rozenblatt-Rosen, Orit Regev, Aviv |
author_sort | Delorey, Toni M. |
collection | PubMed |
description | The SARS-CoV-2 pandemic has caused over 1 million deaths globally, mostly due to acute lung injury and acute respiratory distress syndrome, or direct complications resulting in multiple-organ failures. Little is known about the host tissue immune and cellular responses associated with COVID-19 infection, symptoms, and lethality. To address this, we collected tissues from 11 organs during the clinical autopsy of 17 individuals who succumbed to COVID-19, resulting in a tissue bank of approximately 420 specimens. We generated comprehensive cellular maps capturing COVID-19 biology related to patients’ demise through single-cell and single-nucleus RNA-Seq of lung, kidney, liver and heart tissues, and further contextualized our findings through spatial RNA profiling of distinct lung regions. We developed a computational framework that incorporates removal of ambient RNA and automated cell type annotation to facilitate comparison with other healthy and diseased tissue atlases. In the lung, we uncovered significantly altered transcriptional programs within the epithelial, immune, and stromal compartments and cell intrinsic changes in multiple cell types relative to lung tissue from healthy controls. We observed evidence of: alveolar type 2 (AT2) differentiation replacing depleted alveolar type 1 (AT1) lung epithelial cells, as previously seen in fibrosis; a concomitant increase in myofibroblasts reflective of defective tissue repair; and, putative TP63(+) intrapulmonary basal-like progenitor (IPBLP) cells, similar to cells identified in H1N1 influenza, that may serve as an emergency cellular reserve for severely damaged alveoli. Together, these findings suggest the activation and failure of multiple avenues for regeneration of the epithelium in these terminal lungs. SARS-CoV-2 RNA reads were enriched in lung mononuclear phagocytic cells and endothelial cells, and these cells expressed distinct host response transcriptional programs. We corroborated the compositional and transcriptional changes in lung tissue through spatial analysis of RNA profiles in situ and distinguished unique tissue host responses between regions with and without viral RNA, and in COVID-19 donor tissues relative to healthy lung. Finally, we analyzed genetic regions implicated in COVID-19 GWAS with transcriptomic data to implicate specific cell types and genes associated with disease severity. Overall, our COVID-19 cell atlas is a foundational dataset to better understand the biological impact of SARS-CoV-2 infection across the human body and empowers the identification of new therapeutic interventions and prevention strategies. |
format | Online Article Text |
id | pubmed-7924267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-79242672021-03-03 A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2 Delorey, Toni M. Ziegler, Carly G. K. Heimberg, Graham Normand, Rachelly Yang, Yiming Segerstolpe, Asa Abbondanza, Domenic Fleming, Stephen J. Subramanian, Ayshwarya Montoro, Daniel T. Jagadeesh, Karthik A. Dey, Kushal K. Sen, Pritha Slyper, Michal Pita-Juárez, Yered H. Phillips, Devan Bloom-Ackerman, Zohar Barkas, Nick Ganna, Andrea Gomez, James Normandin, Erica Naderi, Pourya Popov, Yury V. Raju, Siddharth S. Niezen, Sebastian Tsai, Linus T.-Y. Siddle, Katherine J. Sud, Malika Tran, Victoria M. Vellarikkal, Shamsudheen K. Amir-Zilberstein, Liat Atri, Deepak S. Beechem, Joseph Brook, Olga R. Chen, Jonathan Divakar, Prajan Dorceus, Phylicia Engreitz, Jesse M. Essene, Adam Fitzgerald, Donna M. Fropf, Robin Gazal, Steven Gould, Joshua Grzyb, John Harvey, Tyler Hecht, Jonathan Hether, Tyler Jane-Valbuena, Judit Leney-Greene, Michael Ma, Hui McCabe, Cristin McLoughlin, Daniel E. Miller, Eric M. Muus, Christoph Niemi, Mari Padera, Robert Pan, Liuliu Pant, Deepti Pe’er, Carmel Pfiffner-Borges, Jenna Pinto, Christopher J. Plaisted, Jacob Reeves, Jason Ross, Marty Rudy, Melissa Rueckert, Erroll H. Siciliano, Michelle Sturm, Alexander Todres, Ellen Waghray, Avinash Warren, Sarah Zhang, Shuting Zollinger, Daniel R. Cosimi, Lisa Gupta, Rajat M. Hacohen, Nir Hide, Winston Price, Alkes L. Rajagopal, Jayaraj Tata, Purushothama Rao Riedel, Stefan Szabo, Gyongyi Tickle, Timothy L. Hung, Deborah Sabeti, Pardis C. Novak, Richard Rogers, Robert Ingber, Donald E. Jiang, Z. Gordon Juric, Dejan Babadi, Mehrtash Farhi, Samouil L. Stone, James R. Vlachos, Ioannis S. Solomon, Isaac H. Ashenberg, Orr Porter, Caroline B.M. Li, Bo Shalek, Alex K. Villani, Alexandra-Chloé Rozenblatt-Rosen, Orit Regev, Aviv bioRxiv Article The SARS-CoV-2 pandemic has caused over 1 million deaths globally, mostly due to acute lung injury and acute respiratory distress syndrome, or direct complications resulting in multiple-organ failures. Little is known about the host tissue immune and cellular responses associated with COVID-19 infection, symptoms, and lethality. To address this, we collected tissues from 11 organs during the clinical autopsy of 17 individuals who succumbed to COVID-19, resulting in a tissue bank of approximately 420 specimens. We generated comprehensive cellular maps capturing COVID-19 biology related to patients’ demise through single-cell and single-nucleus RNA-Seq of lung, kidney, liver and heart tissues, and further contextualized our findings through spatial RNA profiling of distinct lung regions. We developed a computational framework that incorporates removal of ambient RNA and automated cell type annotation to facilitate comparison with other healthy and diseased tissue atlases. In the lung, we uncovered significantly altered transcriptional programs within the epithelial, immune, and stromal compartments and cell intrinsic changes in multiple cell types relative to lung tissue from healthy controls. We observed evidence of: alveolar type 2 (AT2) differentiation replacing depleted alveolar type 1 (AT1) lung epithelial cells, as previously seen in fibrosis; a concomitant increase in myofibroblasts reflective of defective tissue repair; and, putative TP63(+) intrapulmonary basal-like progenitor (IPBLP) cells, similar to cells identified in H1N1 influenza, that may serve as an emergency cellular reserve for severely damaged alveoli. Together, these findings suggest the activation and failure of multiple avenues for regeneration of the epithelium in these terminal lungs. SARS-CoV-2 RNA reads were enriched in lung mononuclear phagocytic cells and endothelial cells, and these cells expressed distinct host response transcriptional programs. We corroborated the compositional and transcriptional changes in lung tissue through spatial analysis of RNA profiles in situ and distinguished unique tissue host responses between regions with and without viral RNA, and in COVID-19 donor tissues relative to healthy lung. Finally, we analyzed genetic regions implicated in COVID-19 GWAS with transcriptomic data to implicate specific cell types and genes associated with disease severity. Overall, our COVID-19 cell atlas is a foundational dataset to better understand the biological impact of SARS-CoV-2 infection across the human body and empowers the identification of new therapeutic interventions and prevention strategies. Cold Spring Harbor Laboratory 2021-02-25 /pmc/articles/PMC7924267/ /pubmed/33655247 http://dx.doi.org/10.1101/2021.02.25.430130 Text en https://creativecommons.org/licenses/by-nd/4.0/This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, and only so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article Delorey, Toni M. Ziegler, Carly G. K. Heimberg, Graham Normand, Rachelly Yang, Yiming Segerstolpe, Asa Abbondanza, Domenic Fleming, Stephen J. Subramanian, Ayshwarya Montoro, Daniel T. Jagadeesh, Karthik A. Dey, Kushal K. Sen, Pritha Slyper, Michal Pita-Juárez, Yered H. Phillips, Devan Bloom-Ackerman, Zohar Barkas, Nick Ganna, Andrea Gomez, James Normandin, Erica Naderi, Pourya Popov, Yury V. Raju, Siddharth S. Niezen, Sebastian Tsai, Linus T.-Y. Siddle, Katherine J. Sud, Malika Tran, Victoria M. Vellarikkal, Shamsudheen K. Amir-Zilberstein, Liat Atri, Deepak S. Beechem, Joseph Brook, Olga R. Chen, Jonathan Divakar, Prajan Dorceus, Phylicia Engreitz, Jesse M. Essene, Adam Fitzgerald, Donna M. Fropf, Robin Gazal, Steven Gould, Joshua Grzyb, John Harvey, Tyler Hecht, Jonathan Hether, Tyler Jane-Valbuena, Judit Leney-Greene, Michael Ma, Hui McCabe, Cristin McLoughlin, Daniel E. Miller, Eric M. Muus, Christoph Niemi, Mari Padera, Robert Pan, Liuliu Pant, Deepti Pe’er, Carmel Pfiffner-Borges, Jenna Pinto, Christopher J. Plaisted, Jacob Reeves, Jason Ross, Marty Rudy, Melissa Rueckert, Erroll H. Siciliano, Michelle Sturm, Alexander Todres, Ellen Waghray, Avinash Warren, Sarah Zhang, Shuting Zollinger, Daniel R. Cosimi, Lisa Gupta, Rajat M. Hacohen, Nir Hide, Winston Price, Alkes L. Rajagopal, Jayaraj Tata, Purushothama Rao Riedel, Stefan Szabo, Gyongyi Tickle, Timothy L. Hung, Deborah Sabeti, Pardis C. Novak, Richard Rogers, Robert Ingber, Donald E. Jiang, Z. Gordon Juric, Dejan Babadi, Mehrtash Farhi, Samouil L. Stone, James R. Vlachos, Ioannis S. Solomon, Isaac H. Ashenberg, Orr Porter, Caroline B.M. Li, Bo Shalek, Alex K. Villani, Alexandra-Chloé Rozenblatt-Rosen, Orit Regev, Aviv A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2 |
title | A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2 |
title_full | A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2 |
title_fullStr | A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2 |
title_full_unstemmed | A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2 |
title_short | A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2 |
title_sort | single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of sars-cov-2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924267/ https://www.ncbi.nlm.nih.gov/pubmed/33655247 http://dx.doi.org/10.1101/2021.02.25.430130 |
work_keys_str_mv | AT deloreytonim asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT zieglercarlygk asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT heimberggraham asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT normandrachelly asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT yangyiming asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT segerstolpeasa asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT abbondanzadomenic asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT flemingstephenj asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT subramanianayshwarya asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT montorodanielt asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT jagadeeshkarthika asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT deykushalk asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT senpritha asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT slypermichal asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT pitajuarezyeredh asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT phillipsdevan asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT bloomackermanzohar asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT barkasnick asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT gannaandrea asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT gomezjames asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT normandinerica asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT naderipourya asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT popovyuryv asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rajusiddharths asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT niezensebastian asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT tsailinusty asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT siddlekatherinej asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT sudmalika asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT tranvictoriam asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT vellarikkalshamsudheenk asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT amirzilbersteinliat asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT atrideepaks asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT beechemjoseph asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT brookolgar asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT chenjonathan asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT divakarprajan asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT dorceusphylicia asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT engreitzjessem asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT esseneadam asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT fitzgeralddonnam asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT fropfrobin asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT gazalsteven asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT gouldjoshua asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT grzybjohn asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT harveytyler asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT hechtjonathan asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT hethertyler asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT janevalbuenajudit asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT leneygreenemichael asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT mahui asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT mccabecristin asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT mcloughlindaniele asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT millerericm asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT muuschristoph asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT niemimari asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT paderarobert asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT panliuliu asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT pantdeepti asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT peercarmel asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT pfiffnerborgesjenna asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT pintochristopherj asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT plaistedjacob asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT reevesjason asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rossmarty asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rudymelissa asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rueckerterrollh asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT sicilianomichelle asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT sturmalexander asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT todresellen asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT waghrayavinash asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT warrensarah asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT zhangshuting asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT zollingerdanielr asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT cosimilisa asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT guptarajatm asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT hacohennir asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT hidewinston asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT pricealkesl asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rajagopaljayaraj asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT tatapurushothamarao asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT riedelstefan asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT szabogyongyi asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT tickletimothyl asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT hungdeborah asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT sabetipardisc asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT novakrichard asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rogersrobert asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT ingberdonalde asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT jiangzgordon asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT juricdejan asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT babadimehrtash asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT farhisamouill asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT stonejamesr asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT vlachosioanniss asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT solomonisaach asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT ashenbergorr asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT portercarolinebm asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT libo asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT shalekalexk asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT villanialexandrachloe asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rozenblattrosenorit asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT regevaviv asinglecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT deloreytonim singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT zieglercarlygk singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT heimberggraham singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT normandrachelly singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT yangyiming singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT segerstolpeasa singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT abbondanzadomenic singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT flemingstephenj singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT subramanianayshwarya singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT montorodanielt singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT jagadeeshkarthika singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT deykushalk singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT senpritha singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT slypermichal singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT pitajuarezyeredh singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT phillipsdevan singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT bloomackermanzohar singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT barkasnick singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT gannaandrea singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT gomezjames singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT normandinerica singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT naderipourya singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT popovyuryv singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rajusiddharths singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT niezensebastian singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT tsailinusty singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT siddlekatherinej singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT sudmalika singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT tranvictoriam singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT vellarikkalshamsudheenk singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT amirzilbersteinliat singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT atrideepaks singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT beechemjoseph singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT brookolgar singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT chenjonathan singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT divakarprajan singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT dorceusphylicia singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT engreitzjessem singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT esseneadam singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT fitzgeralddonnam singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT fropfrobin singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT gazalsteven singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT gouldjoshua singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT grzybjohn singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT harveytyler singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT hechtjonathan singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT hethertyler singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT janevalbuenajudit singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT leneygreenemichael singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT mahui singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT mccabecristin singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT mcloughlindaniele singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT millerericm singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT muuschristoph singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT niemimari singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT paderarobert singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT panliuliu singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT pantdeepti singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT peercarmel singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT pfiffnerborgesjenna singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT pintochristopherj singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT plaistedjacob singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT reevesjason singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rossmarty singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rudymelissa singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rueckerterrollh singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT sicilianomichelle singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT sturmalexander singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT todresellen singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT waghrayavinash singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT warrensarah singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT zhangshuting singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT zollingerdanielr singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT cosimilisa singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT guptarajatm singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT hacohennir singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT hidewinston singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT pricealkesl singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rajagopaljayaraj singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT tatapurushothamarao singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT riedelstefan singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT szabogyongyi singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT tickletimothyl singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT hungdeborah singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT sabetipardisc singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT novakrichard singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rogersrobert singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT ingberdonalde singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT jiangzgordon singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT juricdejan singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT babadimehrtash singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT farhisamouill singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT stonejamesr singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT vlachosioanniss singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT solomonisaach singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT ashenbergorr singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT portercarolinebm singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT libo singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT shalekalexk singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT villanialexandrachloe singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT rozenblattrosenorit singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 AT regevaviv singlecellandspatialatlasofautopsytissuesrevealspathologyandcellulartargetsofsarscov2 |