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Distribution and preservation of the components of the engulfment. What is beyond representative genomes?
Engulfment requires the coordinated, targeted synthesis and degradation of peptidoglycan at the leading edge of the engulfing membrane to allow the mother cell to completely engulf the forespore. Proteins such as the DMP and Q:AH complexes in Bacillus subtilis are essential for engulfment, as are a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924749/ https://www.ncbi.nlm.nih.gov/pubmed/33651833 http://dx.doi.org/10.1371/journal.pone.0246651 |
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author | Soto-Avila, Lizeth Merce, Ricardo Ciria Santos, Walter Castañeda, Nori Gutierrez-Ríos, Rosa-María |
author_facet | Soto-Avila, Lizeth Merce, Ricardo Ciria Santos, Walter Castañeda, Nori Gutierrez-Ríos, Rosa-María |
author_sort | Soto-Avila, Lizeth |
collection | PubMed |
description | Engulfment requires the coordinated, targeted synthesis and degradation of peptidoglycan at the leading edge of the engulfing membrane to allow the mother cell to completely engulf the forespore. Proteins such as the DMP and Q:AH complexes in Bacillus subtilis are essential for engulfment, as are a set of accessory proteins including GerM and SpoIIB, among others. Experimental and bioinformatic studies of these proteins in bacteria distinct from Bacillus subtilis indicate that fundamental differences exist regarding the organization and mechanisms used to successfully perform engulfment. As a consequence, the distribution and prevalence of the proteins involved in engulfment and other proteins that participate in different sporulation stages have been studied using bioinformatic approaches. These works are based on the prediction of orthologs in the genomes of representative Firmicutes and have been helpful in tracing hypotheses about the origin and evolution of sporulation genes, some of which have been postulated as sporulation signatures. To date, an extensive study of these signatures outside of the representative Firmicutes is not available. Here, we asked whether phyletic profiles of proteins involved in engulfment can be used as signatures able to describe the sporulation phenotype. We tested this hypothesis in a set of 954 Firmicutes, finding preserved phyletic profiles defining signatures at the genus level. Finally, a phylogenetic reconstruction based on non-redundant phyletic profiles at the family level shows the non-monophyletic origin of these proteins due to gain/loss events along the phylum Firmicutes. |
format | Online Article Text |
id | pubmed-7924749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-79247492021-03-10 Distribution and preservation of the components of the engulfment. What is beyond representative genomes? Soto-Avila, Lizeth Merce, Ricardo Ciria Santos, Walter Castañeda, Nori Gutierrez-Ríos, Rosa-María PLoS One Research Article Engulfment requires the coordinated, targeted synthesis and degradation of peptidoglycan at the leading edge of the engulfing membrane to allow the mother cell to completely engulf the forespore. Proteins such as the DMP and Q:AH complexes in Bacillus subtilis are essential for engulfment, as are a set of accessory proteins including GerM and SpoIIB, among others. Experimental and bioinformatic studies of these proteins in bacteria distinct from Bacillus subtilis indicate that fundamental differences exist regarding the organization and mechanisms used to successfully perform engulfment. As a consequence, the distribution and prevalence of the proteins involved in engulfment and other proteins that participate in different sporulation stages have been studied using bioinformatic approaches. These works are based on the prediction of orthologs in the genomes of representative Firmicutes and have been helpful in tracing hypotheses about the origin and evolution of sporulation genes, some of which have been postulated as sporulation signatures. To date, an extensive study of these signatures outside of the representative Firmicutes is not available. Here, we asked whether phyletic profiles of proteins involved in engulfment can be used as signatures able to describe the sporulation phenotype. We tested this hypothesis in a set of 954 Firmicutes, finding preserved phyletic profiles defining signatures at the genus level. Finally, a phylogenetic reconstruction based on non-redundant phyletic profiles at the family level shows the non-monophyletic origin of these proteins due to gain/loss events along the phylum Firmicutes. Public Library of Science 2021-03-02 /pmc/articles/PMC7924749/ /pubmed/33651833 http://dx.doi.org/10.1371/journal.pone.0246651 Text en © 2021 Soto-Avila et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Soto-Avila, Lizeth Merce, Ricardo Ciria Santos, Walter Castañeda, Nori Gutierrez-Ríos, Rosa-María Distribution and preservation of the components of the engulfment. What is beyond representative genomes? |
title | Distribution and preservation of the components of the engulfment. What is beyond representative genomes? |
title_full | Distribution and preservation of the components of the engulfment. What is beyond representative genomes? |
title_fullStr | Distribution and preservation of the components of the engulfment. What is beyond representative genomes? |
title_full_unstemmed | Distribution and preservation of the components of the engulfment. What is beyond representative genomes? |
title_short | Distribution and preservation of the components of the engulfment. What is beyond representative genomes? |
title_sort | distribution and preservation of the components of the engulfment. what is beyond representative genomes? |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924749/ https://www.ncbi.nlm.nih.gov/pubmed/33651833 http://dx.doi.org/10.1371/journal.pone.0246651 |
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