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Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test

Because Japanese cedar pollen (JCP) contains beta-1,3-d-glucan (BG), there is concern that its lingering presence in the atmosphere, especially during its scattering period, may cause false positives in the factor-G-based Limulus amebocyte lysate (LAL) assay used to test for deep mycosis (i.e., G-te...

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Autores principales: Kanno, Takashi, Kim, Changmin, Yamanaka, Daisuke, Ishibashi, Ken-ichi, Tanaka, Hiroshi, Ohno, Naohito, Adachi, Yoshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924878/
https://www.ncbi.nlm.nih.gov/pubmed/33669963
http://dx.doi.org/10.3390/ijms22042135
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author Kanno, Takashi
Kim, Changmin
Yamanaka, Daisuke
Ishibashi, Ken-ichi
Tanaka, Hiroshi
Ohno, Naohito
Adachi, Yoshiyuki
author_facet Kanno, Takashi
Kim, Changmin
Yamanaka, Daisuke
Ishibashi, Ken-ichi
Tanaka, Hiroshi
Ohno, Naohito
Adachi, Yoshiyuki
author_sort Kanno, Takashi
collection PubMed
description Because Japanese cedar pollen (JCP) contains beta-1,3-d-glucan (BG), there is concern that its lingering presence in the atmosphere, especially during its scattering period, may cause false positives in the factor-G-based Limulus amebocyte lysate (LAL) assay used to test for deep mycosis (i.e., G-test). Hence, we examined whether the LAL assay would react positively with substances contained in JCP by using the G-test to measure JCP particles and extracts. BG was purified from the JCP extract on a BG-specific affinity column, and the percentage extractability was measured using three different BG-specific quantitative methods. The G-test detected 0.4 pg BG in a single JCP particle and 10 fg from a single particle in the extract. The percentage extractability of JCP-derived BG was not significantly different among the three quantitative methods. As the JCP particles should technically have been removed during serum separation, they should be less likely to be a direct false-positive factor. However, given that the LAL-assay-positive substances in the JCP extract were not distinguishable by the three BG-specific quantitative methods, we conclude that they may cause the background to rise. Therefore, in Japan false positives arising from JCP contamination should be considered when testing patients for deep mycosis.
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spelling pubmed-79248782021-03-03 Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test Kanno, Takashi Kim, Changmin Yamanaka, Daisuke Ishibashi, Ken-ichi Tanaka, Hiroshi Ohno, Naohito Adachi, Yoshiyuki Int J Mol Sci Article Because Japanese cedar pollen (JCP) contains beta-1,3-d-glucan (BG), there is concern that its lingering presence in the atmosphere, especially during its scattering period, may cause false positives in the factor-G-based Limulus amebocyte lysate (LAL) assay used to test for deep mycosis (i.e., G-test). Hence, we examined whether the LAL assay would react positively with substances contained in JCP by using the G-test to measure JCP particles and extracts. BG was purified from the JCP extract on a BG-specific affinity column, and the percentage extractability was measured using three different BG-specific quantitative methods. The G-test detected 0.4 pg BG in a single JCP particle and 10 fg from a single particle in the extract. The percentage extractability of JCP-derived BG was not significantly different among the three quantitative methods. As the JCP particles should technically have been removed during serum separation, they should be less likely to be a direct false-positive factor. However, given that the LAL-assay-positive substances in the JCP extract were not distinguishable by the three BG-specific quantitative methods, we conclude that they may cause the background to rise. Therefore, in Japan false positives arising from JCP contamination should be considered when testing patients for deep mycosis. MDPI 2021-02-21 /pmc/articles/PMC7924878/ /pubmed/33669963 http://dx.doi.org/10.3390/ijms22042135 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kanno, Takashi
Kim, Changmin
Yamanaka, Daisuke
Ishibashi, Ken-ichi
Tanaka, Hiroshi
Ohno, Naohito
Adachi, Yoshiyuki
Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test
title Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test
title_full Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test
title_fullStr Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test
title_full_unstemmed Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test
title_short Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test
title_sort possibility of japanese cedar pollen causing false positives in the deep mycosis test
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924878/
https://www.ncbi.nlm.nih.gov/pubmed/33669963
http://dx.doi.org/10.3390/ijms22042135
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