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A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature
Microvascular dysfunction plays a fundamental role in the pathogenesis of salivary gland disorders. Restoring and preserving microvascular integrity might therefore represent a promising strategy for the treatment of these pathologies. The mechanisms underlying microvascular dysfunction in salivary...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925411/ https://www.ncbi.nlm.nih.gov/pubmed/33679695 http://dx.doi.org/10.3389/fimmu.2020.604470 |
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author | Uhl, Bernd Braun, Constanze Dominik, Julian Luft, Joshua Canis, Martin Reichel, Christoph A. |
author_facet | Uhl, Bernd Braun, Constanze Dominik, Julian Luft, Joshua Canis, Martin Reichel, Christoph A. |
author_sort | Uhl, Bernd |
collection | PubMed |
description | Microvascular dysfunction plays a fundamental role in the pathogenesis of salivary gland disorders. Restoring and preserving microvascular integrity might therefore represent a promising strategy for the treatment of these pathologies. The mechanisms underlying microvascular dysfunction in salivary glands, however, are still obscure, partly due to the unavailability of adequate in vivo models. Here, we present a novel experimental approach that allows comprehensive in vivo analyses of the salivary gland microvasculature in mice. For this purpose, we employed different microscopy techniques including multi-photon in vivo microscopy to quantitatively analyze interactions of distinct immune cell subsets in the submandibular gland microvasculature required for their infiltration into the surrounding parenchyma and their effects on microvascular function. Confocal microscopy and multi-channel flow cytometry in tissue sections/homogenates complemented these real-time analyses by determining the molecular phenotype of the participating cells. To this end, we identified key adhesion and signaling molecules that regulate the subset- and tissue-specific trafficking of leukocytes into inflamed glands and control the associated microvascular leakage. Hence, we established an experimental approach that allows in vivo analyses of microvascular processes in healthy and diseased salivary glands. This enables us to delineate distinct pathogenetic factors as novel therapeutic targets in salivary gland diseases. |
format | Online Article Text |
id | pubmed-7925411 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79254112021-03-04 A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature Uhl, Bernd Braun, Constanze Dominik, Julian Luft, Joshua Canis, Martin Reichel, Christoph A. Front Immunol Immunology Microvascular dysfunction plays a fundamental role in the pathogenesis of salivary gland disorders. Restoring and preserving microvascular integrity might therefore represent a promising strategy for the treatment of these pathologies. The mechanisms underlying microvascular dysfunction in salivary glands, however, are still obscure, partly due to the unavailability of adequate in vivo models. Here, we present a novel experimental approach that allows comprehensive in vivo analyses of the salivary gland microvasculature in mice. For this purpose, we employed different microscopy techniques including multi-photon in vivo microscopy to quantitatively analyze interactions of distinct immune cell subsets in the submandibular gland microvasculature required for their infiltration into the surrounding parenchyma and their effects on microvascular function. Confocal microscopy and multi-channel flow cytometry in tissue sections/homogenates complemented these real-time analyses by determining the molecular phenotype of the participating cells. To this end, we identified key adhesion and signaling molecules that regulate the subset- and tissue-specific trafficking of leukocytes into inflamed glands and control the associated microvascular leakage. Hence, we established an experimental approach that allows in vivo analyses of microvascular processes in healthy and diseased salivary glands. This enables us to delineate distinct pathogenetic factors as novel therapeutic targets in salivary gland diseases. Frontiers Media S.A. 2021-02-17 /pmc/articles/PMC7925411/ /pubmed/33679695 http://dx.doi.org/10.3389/fimmu.2020.604470 Text en Copyright © 2021 Uhl, Braun, Dominik, Luft, Canis and Reichel http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Uhl, Bernd Braun, Constanze Dominik, Julian Luft, Joshua Canis, Martin Reichel, Christoph A. A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature |
title | A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature |
title_full | A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature |
title_fullStr | A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature |
title_full_unstemmed | A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature |
title_short | A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature |
title_sort | novel experimental approach for in vivo analyses of the salivary gland microvasculature |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925411/ https://www.ncbi.nlm.nih.gov/pubmed/33679695 http://dx.doi.org/10.3389/fimmu.2020.604470 |
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