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The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows

Physiological balance between pro- and antioxidative processes is crucial for placentation and further development of fetus and placenta. Parameters of pro- and antioxidative profile may serve as markers of proper course of pregnancy. The aim of study was to assess whether the balance between pro- a...

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Autores principales: Wawrzykowski, Jacek, Jamioł, Monika, Mojsym, Wioleta, Kankofer, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925458/
https://www.ncbi.nlm.nih.gov/pubmed/33507474
http://dx.doi.org/10.1007/s11033-021-06143-0
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author Wawrzykowski, Jacek
Jamioł, Monika
Mojsym, Wioleta
Kankofer, Marta
author_facet Wawrzykowski, Jacek
Jamioł, Monika
Mojsym, Wioleta
Kankofer, Marta
author_sort Wawrzykowski, Jacek
collection PubMed
description Physiological balance between pro- and antioxidative processes is crucial for placentation and further development of fetus and placenta. Parameters of pro- and antioxidative profile may serve as markers of proper course of pregnancy. The aim of study was to assess whether the balance between pro- and antioxidative parameters during placentation phase in bovine placenta is maintained. Placental and blood samples were collected from healthy, HF, pregnant (2nd-3rd month) cows (n = 8) in slaughterhouse and in farm, respectively. Formylokinurenine and bityrosine content were measured spectrofluorimetrically in blood plasma and tissue homogenates while metabolites of lipid peroxidation, total antioxidant capacity, SH groups and activity of antioxidative enzymes (glutathione peroxidase and superoxide dismutase) were determined in examined tissues by spectrophotometry. Western blotting was used to confirm the presence of enzymatic proteins in placenta. Results: Local profile in tissues was more pronounced than general profile in blood plasma. Activities of antioxidative enzymes were significantly (p < 0.05) higher in 2nd compared to 3rd month of pregnancy in maternal part of placenta while prooxidant parameters showed opposite relationship. Obtained results showed significant differences when compared to data from non-pregnant animals or time of parturition. Further studies are necessary for elucidation of placentation phase in cows.
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spelling pubmed-79254582021-03-19 The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows Wawrzykowski, Jacek Jamioł, Monika Mojsym, Wioleta Kankofer, Marta Mol Biol Rep Original Article Physiological balance between pro- and antioxidative processes is crucial for placentation and further development of fetus and placenta. Parameters of pro- and antioxidative profile may serve as markers of proper course of pregnancy. The aim of study was to assess whether the balance between pro- and antioxidative parameters during placentation phase in bovine placenta is maintained. Placental and blood samples were collected from healthy, HF, pregnant (2nd-3rd month) cows (n = 8) in slaughterhouse and in farm, respectively. Formylokinurenine and bityrosine content were measured spectrofluorimetrically in blood plasma and tissue homogenates while metabolites of lipid peroxidation, total antioxidant capacity, SH groups and activity of antioxidative enzymes (glutathione peroxidase and superoxide dismutase) were determined in examined tissues by spectrophotometry. Western blotting was used to confirm the presence of enzymatic proteins in placenta. Results: Local profile in tissues was more pronounced than general profile in blood plasma. Activities of antioxidative enzymes were significantly (p < 0.05) higher in 2nd compared to 3rd month of pregnancy in maternal part of placenta while prooxidant parameters showed opposite relationship. Obtained results showed significant differences when compared to data from non-pregnant animals or time of parturition. Further studies are necessary for elucidation of placentation phase in cows. Springer Netherlands 2021-01-28 2021 /pmc/articles/PMC7925458/ /pubmed/33507474 http://dx.doi.org/10.1007/s11033-021-06143-0 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Wawrzykowski, Jacek
Jamioł, Monika
Mojsym, Wioleta
Kankofer, Marta
The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows
title The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows
title_full The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows
title_fullStr The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows
title_full_unstemmed The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows
title_short The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows
title_sort comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925458/
https://www.ncbi.nlm.nih.gov/pubmed/33507474
http://dx.doi.org/10.1007/s11033-021-06143-0
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