An improved method for precise genome editing in zebrafish using CRISPR-Cas9 technique

Current methods of CRISPR-Cas9-mediated site-specific mutagenesis create deletions and small insertions at the target site which are repaired by imprecise non-homologous end-joining. Targeting of the Cas9 nuclease relies on a short guide RNA (gRNA) corresponding to the genome sequence approximately...

Descripción completa

Detalles Bibliográficos
Autores principales: Gasanov, Eugene V., Jędrychowska, Justyna, Pastor, Michal, Wiweger, Malgorzata, Methner, Axel, Korzh, Vladimir P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925485/
https://www.ncbi.nlm.nih.gov/pubmed/33481178
http://dx.doi.org/10.1007/s11033-020-06125-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925485/
https://www.ncbi.nlm.nih.gov/pubmed/33481178
http://dx.doi.org/10.1007/s11033-020-06125-8

Ejemplares similares