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Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy

We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma. FLIM can be used to monitor or detect changes in the fluorescence lifetime of metabolic cofactors (e.g. N...

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Autores principales: Hinsdale, Taylor A., Malik, Bilal H., Cheng, Shuna, Benavides, Oscar R., Giger, Maryellen L., Wright, John M., Patel, Paras B., Jo, Javier A., Maitland, Kristen C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925521/
https://www.ncbi.nlm.nih.gov/pubmed/33654229
http://dx.doi.org/10.1038/s41598-021-84552-8
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author Hinsdale, Taylor A.
Malik, Bilal H.
Cheng, Shuna
Benavides, Oscar R.
Giger, Maryellen L.
Wright, John M.
Patel, Paras B.
Jo, Javier A.
Maitland, Kristen C.
author_facet Hinsdale, Taylor A.
Malik, Bilal H.
Cheng, Shuna
Benavides, Oscar R.
Giger, Maryellen L.
Wright, John M.
Patel, Paras B.
Jo, Javier A.
Maitland, Kristen C.
author_sort Hinsdale, Taylor A.
collection PubMed
description We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma. FLIM can be used to monitor or detect changes in the fluorescence lifetime of metabolic cofactors (e.g. NADH and FAD) associated with the onset of carcinogenesis. However, out of focus fluorescence often interferes with this lifetime measurement. Structured illumination fluorescence lifetime imaging (SI-FLIM) addresses this by providing depth-resolved lifetime measurements, and applied to oral mucosa, can localize the collected signal to the epithelium. In this study, the hamster model of oral carcinogenesis was used to evaluate SI-FLIM in premalignant and malignant oral mucosa. Cheek pouches were imaged in vivo and correlated to histopathological diagnoses. The potential of NADH fluorescence signal and lifetime, as measured by widefield FLIM and SI-FLIM, to differentiate dysplasia (pre-malignancy) from normal tissue was evaluated. ROC analysis was carried out with the task of discriminating between normal tissue and mild dysplasia, when changes in fluorescence characteristics are localized to the epithelium only. The results demonstrate that SI-FLIM (AUC = 0.83) is a significantly better (p-value = 0.031) marker for mild dysplasia when compared to widefield FLIM (AUC = 0.63).
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spelling pubmed-79255212021-03-04 Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy Hinsdale, Taylor A. Malik, Bilal H. Cheng, Shuna Benavides, Oscar R. Giger, Maryellen L. Wright, John M. Patel, Paras B. Jo, Javier A. Maitland, Kristen C. Sci Rep Article We demonstrate that structured illumination microscopy has the potential to enhance fluorescence lifetime imaging microscopy (FLIM) as an early detection method for oral squamous cell carcinoma. FLIM can be used to monitor or detect changes in the fluorescence lifetime of metabolic cofactors (e.g. NADH and FAD) associated with the onset of carcinogenesis. However, out of focus fluorescence often interferes with this lifetime measurement. Structured illumination fluorescence lifetime imaging (SI-FLIM) addresses this by providing depth-resolved lifetime measurements, and applied to oral mucosa, can localize the collected signal to the epithelium. In this study, the hamster model of oral carcinogenesis was used to evaluate SI-FLIM in premalignant and malignant oral mucosa. Cheek pouches were imaged in vivo and correlated to histopathological diagnoses. The potential of NADH fluorescence signal and lifetime, as measured by widefield FLIM and SI-FLIM, to differentiate dysplasia (pre-malignancy) from normal tissue was evaluated. ROC analysis was carried out with the task of discriminating between normal tissue and mild dysplasia, when changes in fluorescence characteristics are localized to the epithelium only. The results demonstrate that SI-FLIM (AUC = 0.83) is a significantly better (p-value = 0.031) marker for mild dysplasia when compared to widefield FLIM (AUC = 0.63). Nature Publishing Group UK 2021-03-02 /pmc/articles/PMC7925521/ /pubmed/33654229 http://dx.doi.org/10.1038/s41598-021-84552-8 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hinsdale, Taylor A.
Malik, Bilal H.
Cheng, Shuna
Benavides, Oscar R.
Giger, Maryellen L.
Wright, John M.
Patel, Paras B.
Jo, Javier A.
Maitland, Kristen C.
Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
title Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
title_full Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
title_fullStr Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
title_full_unstemmed Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
title_short Enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
title_sort enhanced detection of oral dysplasia by structured illumination fluorescence lifetime imaging microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925521/
https://www.ncbi.nlm.nih.gov/pubmed/33654229
http://dx.doi.org/10.1038/s41598-021-84552-8
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