Cargando…
Isolation and Pathogenic Characterization of Pigeon Paramyxovirus Type 1 via Different Inoculation Routes in Pigeons
Pigeon paramyxovirus type I (PPMV-1) causes regular outbreaks in pigeons and even poses a pandemic threat among chickens and other birds. The birds infected with PPMV-1 mainly show a pathological damage in the respiratory system, digestive system, and nervous system. However, there were few reports...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925627/ https://www.ncbi.nlm.nih.gov/pubmed/33681314 http://dx.doi.org/10.3389/fvets.2020.569901 |
_version_ | 1783659310638694400 |
---|---|
author | Chang, Han Feng, Shengyong Wang, Yutian Li, Fuhuang Su, Qianqian Wang, Bo Du, Juan He, Hongxuan |
author_facet | Chang, Han Feng, Shengyong Wang, Yutian Li, Fuhuang Su, Qianqian Wang, Bo Du, Juan He, Hongxuan |
author_sort | Chang, Han |
collection | PubMed |
description | Pigeon paramyxovirus type I (PPMV-1) causes regular outbreaks in pigeons and even poses a pandemic threat among chickens and other birds. The birds infected with PPMV-1 mainly show a pathological damage in the respiratory system, digestive system, and nervous system. However, there were few reports on the efficiency of the virus entering the host via routes of different systems. In the present study, a PPMV-1 strain was obtained from a dead wild pigeon in 2016 in Beijing, China. The mean death time (MDT) and the intracerebral pathogenicity (ICPI) of our isolate showed medium virulence. Phylogenetic analysis based on F gene sequence showed that the isolate belonged to subgenotype VIb, class II, which dominated in China in recent years. Then, we evaluated the infection efficiency of different routes. Pigeons were randomly divided into five groups of six as follows: intracephalic (IC), intranasal (IN), and intraoral (IO) infection routes, cohabitation infection (CO), and negative control (N negative). All pigeons were inoculated with 100 μl·10(6) EID(50) PPMV-1 virus. After infection, pathological lesions, virus shedding, body weight change, survival rate, and tissue tropism were tested to compare the efficiency of the different infected routes. The mortality of groups IC, IN, IO, and CO were 100, 66.7, 50, and 33.3%, respectively. Weight loss in group IC was higher than the other groups, followed by groups IN and IO. The lesions observed in PPMV-1-infected pigeons were severe, especially in the lung and intestine in group IC. Viral shedding was observed from 2 dpi in groups IC and IN, but the shedding rate was higher in group IN than group IC. The longest period was in group CO. Tissue tropism experiment showed that our isolate has a wide range of tissue distribution, and the virus titer in the heart and intestine of group IC and in the brain of group IN was higher. Our data may help us to evaluate the risk of transmission of PPMV-1. |
format | Online Article Text |
id | pubmed-7925627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79256272021-03-04 Isolation and Pathogenic Characterization of Pigeon Paramyxovirus Type 1 via Different Inoculation Routes in Pigeons Chang, Han Feng, Shengyong Wang, Yutian Li, Fuhuang Su, Qianqian Wang, Bo Du, Juan He, Hongxuan Front Vet Sci Veterinary Science Pigeon paramyxovirus type I (PPMV-1) causes regular outbreaks in pigeons and even poses a pandemic threat among chickens and other birds. The birds infected with PPMV-1 mainly show a pathological damage in the respiratory system, digestive system, and nervous system. However, there were few reports on the efficiency of the virus entering the host via routes of different systems. In the present study, a PPMV-1 strain was obtained from a dead wild pigeon in 2016 in Beijing, China. The mean death time (MDT) and the intracerebral pathogenicity (ICPI) of our isolate showed medium virulence. Phylogenetic analysis based on F gene sequence showed that the isolate belonged to subgenotype VIb, class II, which dominated in China in recent years. Then, we evaluated the infection efficiency of different routes. Pigeons were randomly divided into five groups of six as follows: intracephalic (IC), intranasal (IN), and intraoral (IO) infection routes, cohabitation infection (CO), and negative control (N negative). All pigeons were inoculated with 100 μl·10(6) EID(50) PPMV-1 virus. After infection, pathological lesions, virus shedding, body weight change, survival rate, and tissue tropism were tested to compare the efficiency of the different infected routes. The mortality of groups IC, IN, IO, and CO were 100, 66.7, 50, and 33.3%, respectively. Weight loss in group IC was higher than the other groups, followed by groups IN and IO. The lesions observed in PPMV-1-infected pigeons were severe, especially in the lung and intestine in group IC. Viral shedding was observed from 2 dpi in groups IC and IN, but the shedding rate was higher in group IN than group IC. The longest period was in group CO. Tissue tropism experiment showed that our isolate has a wide range of tissue distribution, and the virus titer in the heart and intestine of group IC and in the brain of group IN was higher. Our data may help us to evaluate the risk of transmission of PPMV-1. Frontiers Media S.A. 2021-02-17 /pmc/articles/PMC7925627/ /pubmed/33681314 http://dx.doi.org/10.3389/fvets.2020.569901 Text en Copyright © 2021 Chang, Feng, Wang, Li, Su, Wang, Du and He. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Chang, Han Feng, Shengyong Wang, Yutian Li, Fuhuang Su, Qianqian Wang, Bo Du, Juan He, Hongxuan Isolation and Pathogenic Characterization of Pigeon Paramyxovirus Type 1 via Different Inoculation Routes in Pigeons |
title | Isolation and Pathogenic Characterization of Pigeon Paramyxovirus Type 1 via Different Inoculation Routes in Pigeons |
title_full | Isolation and Pathogenic Characterization of Pigeon Paramyxovirus Type 1 via Different Inoculation Routes in Pigeons |
title_fullStr | Isolation and Pathogenic Characterization of Pigeon Paramyxovirus Type 1 via Different Inoculation Routes in Pigeons |
title_full_unstemmed | Isolation and Pathogenic Characterization of Pigeon Paramyxovirus Type 1 via Different Inoculation Routes in Pigeons |
title_short | Isolation and Pathogenic Characterization of Pigeon Paramyxovirus Type 1 via Different Inoculation Routes in Pigeons |
title_sort | isolation and pathogenic characterization of pigeon paramyxovirus type 1 via different inoculation routes in pigeons |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925627/ https://www.ncbi.nlm.nih.gov/pubmed/33681314 http://dx.doi.org/10.3389/fvets.2020.569901 |
work_keys_str_mv | AT changhan isolationandpathogeniccharacterizationofpigeonparamyxovirustype1viadifferentinoculationroutesinpigeons AT fengshengyong isolationandpathogeniccharacterizationofpigeonparamyxovirustype1viadifferentinoculationroutesinpigeons AT wangyutian isolationandpathogeniccharacterizationofpigeonparamyxovirustype1viadifferentinoculationroutesinpigeons AT lifuhuang isolationandpathogeniccharacterizationofpigeonparamyxovirustype1viadifferentinoculationroutesinpigeons AT suqianqian isolationandpathogeniccharacterizationofpigeonparamyxovirustype1viadifferentinoculationroutesinpigeons AT wangbo isolationandpathogeniccharacterizationofpigeonparamyxovirustype1viadifferentinoculationroutesinpigeons AT dujuan isolationandpathogeniccharacterizationofpigeonparamyxovirustype1viadifferentinoculationroutesinpigeons AT hehongxuan isolationandpathogeniccharacterizationofpigeonparamyxovirustype1viadifferentinoculationroutesinpigeons |