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Actually Seeing What Is Going on – Intravital Microscopy in Tissue Engineering
Intravital microscopy (IVM) study approach offers several advantages over in vitro, ex vivo, and 3D models. IVM provides real-time imaging of cellular events, which provides us a comprehensive picture of dynamic processes. Rapid improvement in microscopy techniques has permitted deep tissue imaging...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925911/ https://www.ncbi.nlm.nih.gov/pubmed/33681162 http://dx.doi.org/10.3389/fbioe.2021.627462 |
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author | Vaghela, Ravikumar Arkudas, Andreas Horch, Raymund E. Hessenauer, Maximilian |
author_facet | Vaghela, Ravikumar Arkudas, Andreas Horch, Raymund E. Hessenauer, Maximilian |
author_sort | Vaghela, Ravikumar |
collection | PubMed |
description | Intravital microscopy (IVM) study approach offers several advantages over in vitro, ex vivo, and 3D models. IVM provides real-time imaging of cellular events, which provides us a comprehensive picture of dynamic processes. Rapid improvement in microscopy techniques has permitted deep tissue imaging at a higher resolution. Advances in fluorescence tagging methods enable tracking of specific cell types. Moreover, IVM can serve as an important tool to study different stages of tissue regeneration processes. Furthermore, the compatibility of different tissue engineered constructs can be analyzed. IVM is also a promising approach to investigate host reactions on implanted biomaterials. IVM can provide instant feedback for improvising tissue engineering strategies. In this review, we aim to provide an overview of the requirements and applications of different IVM approaches. First, we will discuss the history of IVM development, and then we will provide an overview of available optical modalities including the pros and cons. Later, we will summarize different fluorescence labeling methods. In the final section, we will discuss well-established chronic and acute IVM models for different organs. |
format | Online Article Text |
id | pubmed-7925911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79259112021-03-04 Actually Seeing What Is Going on – Intravital Microscopy in Tissue Engineering Vaghela, Ravikumar Arkudas, Andreas Horch, Raymund E. Hessenauer, Maximilian Front Bioeng Biotechnol Bioengineering and Biotechnology Intravital microscopy (IVM) study approach offers several advantages over in vitro, ex vivo, and 3D models. IVM provides real-time imaging of cellular events, which provides us a comprehensive picture of dynamic processes. Rapid improvement in microscopy techniques has permitted deep tissue imaging at a higher resolution. Advances in fluorescence tagging methods enable tracking of specific cell types. Moreover, IVM can serve as an important tool to study different stages of tissue regeneration processes. Furthermore, the compatibility of different tissue engineered constructs can be analyzed. IVM is also a promising approach to investigate host reactions on implanted biomaterials. IVM can provide instant feedback for improvising tissue engineering strategies. In this review, we aim to provide an overview of the requirements and applications of different IVM approaches. First, we will discuss the history of IVM development, and then we will provide an overview of available optical modalities including the pros and cons. Later, we will summarize different fluorescence labeling methods. In the final section, we will discuss well-established chronic and acute IVM models for different organs. Frontiers Media S.A. 2021-02-17 /pmc/articles/PMC7925911/ /pubmed/33681162 http://dx.doi.org/10.3389/fbioe.2021.627462 Text en Copyright © 2021 Vaghela, Arkudas, Horch and Hessenauer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Vaghela, Ravikumar Arkudas, Andreas Horch, Raymund E. Hessenauer, Maximilian Actually Seeing What Is Going on – Intravital Microscopy in Tissue Engineering |
title | Actually Seeing What Is Going on – Intravital Microscopy in Tissue Engineering |
title_full | Actually Seeing What Is Going on – Intravital Microscopy in Tissue Engineering |
title_fullStr | Actually Seeing What Is Going on – Intravital Microscopy in Tissue Engineering |
title_full_unstemmed | Actually Seeing What Is Going on – Intravital Microscopy in Tissue Engineering |
title_short | Actually Seeing What Is Going on – Intravital Microscopy in Tissue Engineering |
title_sort | actually seeing what is going on – intravital microscopy in tissue engineering |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925911/ https://www.ncbi.nlm.nih.gov/pubmed/33681162 http://dx.doi.org/10.3389/fbioe.2021.627462 |
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