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Vector-Borne Pathogens with Veterinary and Public Health Significance in Melophagus ovinus (Sheep Ked) from the Qinghai-Tibet Plateau

Melophagus ovinus (sheep ked) is a hematophagous ectoparasite that mainly parasitizes sheep. In addition to causing inflammation, wool loss, and skin damage to the animal hosts, M. ovinus also serves as a vector for a variety of pathogens and is highly likely to participate in the life and transmiss...

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Autores principales: Zhang, Qing-Xun, Wang, Ye, Li, Ying, Han, Shu-Yi, Wang, Bo, Yuan, Guo-Hui, Zhang, Pei-Yang, Yang, Zi-Wen, Wang, Shuang-Ling, Chen, Ji-Yong, Zhong, Hai-Shun, Han, Xue-Qing, He, Hong-Xuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926330/
https://www.ncbi.nlm.nih.gov/pubmed/33671750
http://dx.doi.org/10.3390/pathogens10020249
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author Zhang, Qing-Xun
Wang, Ye
Li, Ying
Han, Shu-Yi
Wang, Bo
Yuan, Guo-Hui
Zhang, Pei-Yang
Yang, Zi-Wen
Wang, Shuang-Ling
Chen, Ji-Yong
Zhong, Hai-Shun
Han, Xue-Qing
He, Hong-Xuan
author_facet Zhang, Qing-Xun
Wang, Ye
Li, Ying
Han, Shu-Yi
Wang, Bo
Yuan, Guo-Hui
Zhang, Pei-Yang
Yang, Zi-Wen
Wang, Shuang-Ling
Chen, Ji-Yong
Zhong, Hai-Shun
Han, Xue-Qing
He, Hong-Xuan
author_sort Zhang, Qing-Xun
collection PubMed
description Melophagus ovinus (sheep ked) is a hematophagous ectoparasite that mainly parasitizes sheep. In addition to causing inflammation, wool loss, and skin damage to the animal hosts, M. ovinus also serves as a vector for a variety of pathogens and is highly likely to participate in the life and transmission cycle of pathogenic organisms. Herein, we investigated the presence and molecular characterization of vector-borne pathogens in M. ovinus from Qinghai-Tibet Plateau, China. A total of 92 M. ovinus pools collected from the Qinghai province of China were screened for the presence of selected vector-borne pathogens. The overall positive rate of A. ovis, A. bovis, A. phagocytophilum, and T. ovis in M. ovinus was 39.1%, 17.4%, 9.8%, and 89.1%, respectively. All of the samples were negative for Border disease virus (BDV), other Anaplasma species, Babesia spp., Rickettsia spp., and Borrelia spp. Co-infection of different Anaplasma species and T. ovis occurred in 51.2% of all samples with T. ovis. The positive rates of A. ovis, A. bovis, and A. phagocytophilum in different regions and altitudes of the sampling sites were significantly different. Sequence and phylogenetic analysis of target genes confirmed their identity with corresponding pathogens. Our results elucidate the occurrence and molecular characterization of Anaplasma spp. and Theileria spp. in M. ovinus, which could act as potential zoonotic reservoirs. To the best of our knowledge, this is the first report of the detection of A. bovis and A. phagocytophilum DNA in M. ovinus. This study gives the first extensive molecular survey of vector-borne pathogens with veterinary and public health significance in M. ovinus from the Qinghai-Tibet Plateau, China.
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spelling pubmed-79263302021-03-04 Vector-Borne Pathogens with Veterinary and Public Health Significance in Melophagus ovinus (Sheep Ked) from the Qinghai-Tibet Plateau Zhang, Qing-Xun Wang, Ye Li, Ying Han, Shu-Yi Wang, Bo Yuan, Guo-Hui Zhang, Pei-Yang Yang, Zi-Wen Wang, Shuang-Ling Chen, Ji-Yong Zhong, Hai-Shun Han, Xue-Qing He, Hong-Xuan Pathogens Article Melophagus ovinus (sheep ked) is a hematophagous ectoparasite that mainly parasitizes sheep. In addition to causing inflammation, wool loss, and skin damage to the animal hosts, M. ovinus also serves as a vector for a variety of pathogens and is highly likely to participate in the life and transmission cycle of pathogenic organisms. Herein, we investigated the presence and molecular characterization of vector-borne pathogens in M. ovinus from Qinghai-Tibet Plateau, China. A total of 92 M. ovinus pools collected from the Qinghai province of China were screened for the presence of selected vector-borne pathogens. The overall positive rate of A. ovis, A. bovis, A. phagocytophilum, and T. ovis in M. ovinus was 39.1%, 17.4%, 9.8%, and 89.1%, respectively. All of the samples were negative for Border disease virus (BDV), other Anaplasma species, Babesia spp., Rickettsia spp., and Borrelia spp. Co-infection of different Anaplasma species and T. ovis occurred in 51.2% of all samples with T. ovis. The positive rates of A. ovis, A. bovis, and A. phagocytophilum in different regions and altitudes of the sampling sites were significantly different. Sequence and phylogenetic analysis of target genes confirmed their identity with corresponding pathogens. Our results elucidate the occurrence and molecular characterization of Anaplasma spp. and Theileria spp. in M. ovinus, which could act as potential zoonotic reservoirs. To the best of our knowledge, this is the first report of the detection of A. bovis and A. phagocytophilum DNA in M. ovinus. This study gives the first extensive molecular survey of vector-borne pathogens with veterinary and public health significance in M. ovinus from the Qinghai-Tibet Plateau, China. MDPI 2021-02-22 /pmc/articles/PMC7926330/ /pubmed/33671750 http://dx.doi.org/10.3390/pathogens10020249 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Qing-Xun
Wang, Ye
Li, Ying
Han, Shu-Yi
Wang, Bo
Yuan, Guo-Hui
Zhang, Pei-Yang
Yang, Zi-Wen
Wang, Shuang-Ling
Chen, Ji-Yong
Zhong, Hai-Shun
Han, Xue-Qing
He, Hong-Xuan
Vector-Borne Pathogens with Veterinary and Public Health Significance in Melophagus ovinus (Sheep Ked) from the Qinghai-Tibet Plateau
title Vector-Borne Pathogens with Veterinary and Public Health Significance in Melophagus ovinus (Sheep Ked) from the Qinghai-Tibet Plateau
title_full Vector-Borne Pathogens with Veterinary and Public Health Significance in Melophagus ovinus (Sheep Ked) from the Qinghai-Tibet Plateau
title_fullStr Vector-Borne Pathogens with Veterinary and Public Health Significance in Melophagus ovinus (Sheep Ked) from the Qinghai-Tibet Plateau
title_full_unstemmed Vector-Borne Pathogens with Veterinary and Public Health Significance in Melophagus ovinus (Sheep Ked) from the Qinghai-Tibet Plateau
title_short Vector-Borne Pathogens with Veterinary and Public Health Significance in Melophagus ovinus (Sheep Ked) from the Qinghai-Tibet Plateau
title_sort vector-borne pathogens with veterinary and public health significance in melophagus ovinus (sheep ked) from the qinghai-tibet plateau
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926330/
https://www.ncbi.nlm.nih.gov/pubmed/33671750
http://dx.doi.org/10.3390/pathogens10020249
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