Cargando…

Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk

Background: Human milk banks have a pivotal role in provide optimal food for those infants who are not fully breastfeed, by allowing human milk from donors to be collected, processed and appropriately distributed. Donor human milk (DHM) is usually preserved by Holder pasteurization, considered to be...

Descripción completa

Detalles Bibliográficos
Autores principales: Jarzynka, Sylwia, Strom, Kamila, Barbarska, Olga, Pawlikowska, Emilia, Minkiewicz-Zochniak, Anna, Rosiak, Elzbieta, Oledzka, Gabriela, Wesolowska, Aleksandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926441/
https://www.ncbi.nlm.nih.gov/pubmed/33671763
http://dx.doi.org/10.3390/ijerph18042147
_version_ 1783659467025416192
author Jarzynka, Sylwia
Strom, Kamila
Barbarska, Olga
Pawlikowska, Emilia
Minkiewicz-Zochniak, Anna
Rosiak, Elzbieta
Oledzka, Gabriela
Wesolowska, Aleksandra
author_facet Jarzynka, Sylwia
Strom, Kamila
Barbarska, Olga
Pawlikowska, Emilia
Minkiewicz-Zochniak, Anna
Rosiak, Elzbieta
Oledzka, Gabriela
Wesolowska, Aleksandra
author_sort Jarzynka, Sylwia
collection PubMed
description Background: Human milk banks have a pivotal role in provide optimal food for those infants who are not fully breastfeed, by allowing human milk from donors to be collected, processed and appropriately distributed. Donor human milk (DHM) is usually preserved by Holder pasteurization, considered to be the gold standard to ensure the microbiology safety and nutritional value of milk. However, as stated by the European Milk Banking Association (EMBA) there is a need to implement the improvement of the operating procedure of human milk banks including preserving and storing techniques. Aim: The purpose of this study was to assess the effectiveness and safety of the selected new combination of methods for preserving donor human milk in comparison with thermal treatment (Holder pasteurization). Methods: We assessed (1) the concentration of bioactive components (insulin, adiponectin, leptin, activity of pancreatic lipase, and hepatocyte growth factor) and (2) microbiological safety in raw and pasteurized, high-pressure processed and lyophilization human breast milk. Results: The combination of two techniques, high-pressure processing and freeze-drying, showed the best potential for preserving the nutritional value of human milk and were evaluated for microbiological safety. Microbiological safety assessment excluded the possibility of using freeze-drying alone for human milk sample preservation. However, it can be used as a method for long-term storage of milk samples, which have previously been preserved via other processes. Conclusion: The results show that high-pressure treatment is the best method for preservation that ensures microbiological safety and biological activity but subsequent freeze-drying allowed long-term storage without loss of properties.
format Online
Article
Text
id pubmed-7926441
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79264412021-03-04 Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk Jarzynka, Sylwia Strom, Kamila Barbarska, Olga Pawlikowska, Emilia Minkiewicz-Zochniak, Anna Rosiak, Elzbieta Oledzka, Gabriela Wesolowska, Aleksandra Int J Environ Res Public Health Article Background: Human milk banks have a pivotal role in provide optimal food for those infants who are not fully breastfeed, by allowing human milk from donors to be collected, processed and appropriately distributed. Donor human milk (DHM) is usually preserved by Holder pasteurization, considered to be the gold standard to ensure the microbiology safety and nutritional value of milk. However, as stated by the European Milk Banking Association (EMBA) there is a need to implement the improvement of the operating procedure of human milk banks including preserving and storing techniques. Aim: The purpose of this study was to assess the effectiveness and safety of the selected new combination of methods for preserving donor human milk in comparison with thermal treatment (Holder pasteurization). Methods: We assessed (1) the concentration of bioactive components (insulin, adiponectin, leptin, activity of pancreatic lipase, and hepatocyte growth factor) and (2) microbiological safety in raw and pasteurized, high-pressure processed and lyophilization human breast milk. Results: The combination of two techniques, high-pressure processing and freeze-drying, showed the best potential for preserving the nutritional value of human milk and were evaluated for microbiological safety. Microbiological safety assessment excluded the possibility of using freeze-drying alone for human milk sample preservation. However, it can be used as a method for long-term storage of milk samples, which have previously been preserved via other processes. Conclusion: The results show that high-pressure treatment is the best method for preservation that ensures microbiological safety and biological activity but subsequent freeze-drying allowed long-term storage without loss of properties. MDPI 2021-02-22 /pmc/articles/PMC7926441/ /pubmed/33671763 http://dx.doi.org/10.3390/ijerph18042147 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Jarzynka, Sylwia
Strom, Kamila
Barbarska, Olga
Pawlikowska, Emilia
Minkiewicz-Zochniak, Anna
Rosiak, Elzbieta
Oledzka, Gabriela
Wesolowska, Aleksandra
Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk
title Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk
title_full Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk
title_fullStr Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk
title_full_unstemmed Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk
title_short Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk
title_sort combination of high-pressure processing and freeze-drying as the most effective techniques in maintaining biological values and microbiological safety of donor milk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926441/
https://www.ncbi.nlm.nih.gov/pubmed/33671763
http://dx.doi.org/10.3390/ijerph18042147
work_keys_str_mv AT jarzynkasylwia combinationofhighpressureprocessingandfreezedryingasthemosteffectivetechniquesinmaintainingbiologicalvaluesandmicrobiologicalsafetyofdonormilk
AT stromkamila combinationofhighpressureprocessingandfreezedryingasthemosteffectivetechniquesinmaintainingbiologicalvaluesandmicrobiologicalsafetyofdonormilk
AT barbarskaolga combinationofhighpressureprocessingandfreezedryingasthemosteffectivetechniquesinmaintainingbiologicalvaluesandmicrobiologicalsafetyofdonormilk
AT pawlikowskaemilia combinationofhighpressureprocessingandfreezedryingasthemosteffectivetechniquesinmaintainingbiologicalvaluesandmicrobiologicalsafetyofdonormilk
AT minkiewiczzochniakanna combinationofhighpressureprocessingandfreezedryingasthemosteffectivetechniquesinmaintainingbiologicalvaluesandmicrobiologicalsafetyofdonormilk
AT rosiakelzbieta combinationofhighpressureprocessingandfreezedryingasthemosteffectivetechniquesinmaintainingbiologicalvaluesandmicrobiologicalsafetyofdonormilk
AT oledzkagabriela combinationofhighpressureprocessingandfreezedryingasthemosteffectivetechniquesinmaintainingbiologicalvaluesandmicrobiologicalsafetyofdonormilk
AT wesolowskaaleksandra combinationofhighpressureprocessingandfreezedryingasthemosteffectivetechniquesinmaintainingbiologicalvaluesandmicrobiologicalsafetyofdonormilk