Cargando…

Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72(T) Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis

The novel extreme obligate alkaliphilic Bacillus marmarensis DSM 21297 is known to produce polyhydroxybutyrate (PHB). However, the detailed mechanism of PHB synthesis in B. marmarensis is still unknown. Here, we investigated which metabolic pathways and metabolic enzymes are responsible for PHB synt...

Descripción completa

Detalles Bibliográficos
Autores principales: Atakav, Yağmur, Pinar, Orkun, Kazan, Dilek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926669/
https://www.ncbi.nlm.nih.gov/pubmed/33672214
http://dx.doi.org/10.3390/microorganisms9020462
_version_ 1783659514493403136
author Atakav, Yağmur
Pinar, Orkun
Kazan, Dilek
author_facet Atakav, Yağmur
Pinar, Orkun
Kazan, Dilek
author_sort Atakav, Yağmur
collection PubMed
description The novel extreme obligate alkaliphilic Bacillus marmarensis DSM 21297 is known to produce polyhydroxybutyrate (PHB). However, the detailed mechanism of PHB synthesis in B. marmarensis is still unknown. Here, we investigated which metabolic pathways and metabolic enzymes are responsible for PHB synthesis in order to understand the regulatory pathway and optimize PHB synthesis in B. marmarensis. In accordance with the fact that beta-galactosidase, 3-hydroxyacyl-CoA dehydrogenase, and Enoyl-CoA hydratase together with acyl-CoA dehydrogenase and lipase were annotated in B. marmarensis according to the RAST server, we used glucose, lactose, and olive oil to understand the preferred metabolic pathway for the PHB synthesis. It was found that B. marmarensis produces PHB from glucose, lactose, and olive oil. However, the highest PHB titer and the highest amount of PHB synthesized per dry cell mass (Y(P/X)) were achieved in the presence of lactose, as compared to glucose and olive oil. Additionally, in the absence of peptone, the amount of PHB synthesized is reduced for each carbon source. Interestingly, none of the carbon sources studied yielded an efficient PHB synthesis, and supplementation of the medium with potassium ions did not enhance PHB synthesis. According to these experimental results and the presence of annotated metabolic enzymes based on the RAST server, PHB accumulation in the cells of B. marmarensis could be improved by the level of the expression of 3-hydroxybutyryl-CoA dehydrogenase (1.1.1.157), which increases the production of NADPH. Additionally, the accumulation of 3-hydroxyacyl-CoA could enhance the production of PHB in B. marmarensis in the presence of fatty acids. To our knowledge, this is the first report investigating the regulatory system involved in the control of PHB metabolism of B. marmarensis.
format Online
Article
Text
id pubmed-7926669
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79266692021-03-04 Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72(T) Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis Atakav, Yağmur Pinar, Orkun Kazan, Dilek Microorganisms Article The novel extreme obligate alkaliphilic Bacillus marmarensis DSM 21297 is known to produce polyhydroxybutyrate (PHB). However, the detailed mechanism of PHB synthesis in B. marmarensis is still unknown. Here, we investigated which metabolic pathways and metabolic enzymes are responsible for PHB synthesis in order to understand the regulatory pathway and optimize PHB synthesis in B. marmarensis. In accordance with the fact that beta-galactosidase, 3-hydroxyacyl-CoA dehydrogenase, and Enoyl-CoA hydratase together with acyl-CoA dehydrogenase and lipase were annotated in B. marmarensis according to the RAST server, we used glucose, lactose, and olive oil to understand the preferred metabolic pathway for the PHB synthesis. It was found that B. marmarensis produces PHB from glucose, lactose, and olive oil. However, the highest PHB titer and the highest amount of PHB synthesized per dry cell mass (Y(P/X)) were achieved in the presence of lactose, as compared to glucose and olive oil. Additionally, in the absence of peptone, the amount of PHB synthesized is reduced for each carbon source. Interestingly, none of the carbon sources studied yielded an efficient PHB synthesis, and supplementation of the medium with potassium ions did not enhance PHB synthesis. According to these experimental results and the presence of annotated metabolic enzymes based on the RAST server, PHB accumulation in the cells of B. marmarensis could be improved by the level of the expression of 3-hydroxybutyryl-CoA dehydrogenase (1.1.1.157), which increases the production of NADPH. Additionally, the accumulation of 3-hydroxyacyl-CoA could enhance the production of PHB in B. marmarensis in the presence of fatty acids. To our knowledge, this is the first report investigating the regulatory system involved in the control of PHB metabolism of B. marmarensis. MDPI 2021-02-23 /pmc/articles/PMC7926669/ /pubmed/33672214 http://dx.doi.org/10.3390/microorganisms9020462 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Atakav, Yağmur
Pinar, Orkun
Kazan, Dilek
Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72(T) Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis
title Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72(T) Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis
title_full Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72(T) Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis
title_fullStr Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72(T) Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis
title_full_unstemmed Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72(T) Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis
title_short Investigation of the Physiology of the Obligate Alkaliphilic Bacillus marmarensis GMBE 72(T) Considering Its Alkaline Adaptation Mechanism for Poly(3-hydroxybutyrate) Synthesis
title_sort investigation of the physiology of the obligate alkaliphilic bacillus marmarensis gmbe 72(t) considering its alkaline adaptation mechanism for poly(3-hydroxybutyrate) synthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926669/
https://www.ncbi.nlm.nih.gov/pubmed/33672214
http://dx.doi.org/10.3390/microorganisms9020462
work_keys_str_mv AT atakavyagmur investigationofthephysiologyoftheobligatealkaliphilicbacillusmarmarensisgmbe72tconsideringitsalkalineadaptationmechanismforpoly3hydroxybutyratesynthesis
AT pinarorkun investigationofthephysiologyoftheobligatealkaliphilicbacillusmarmarensisgmbe72tconsideringitsalkalineadaptationmechanismforpoly3hydroxybutyratesynthesis
AT kazandilek investigationofthephysiologyoftheobligatealkaliphilicbacillusmarmarensisgmbe72tconsideringitsalkalineadaptationmechanismforpoly3hydroxybutyratesynthesis