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Lipid Droplets Are a Physiological Nucleoporin Reservoir
Lipid Droplets (LD) are dynamic organelles that originate in the Endoplasmic Reticulum and mostly bud off toward the cytoplasm, where they store neutral lipids for energy and protection purposes. LD also have diverse proteins on their surface, many of which are necessary for the their correct homeos...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926788/ https://www.ncbi.nlm.nih.gov/pubmed/33671805 http://dx.doi.org/10.3390/cells10020472 |
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author | Kumanski, Sylvain Viart, Benjamin T. Kossida, Sofia Moriel-Carretero, María |
author_facet | Kumanski, Sylvain Viart, Benjamin T. Kossida, Sofia Moriel-Carretero, María |
author_sort | Kumanski, Sylvain |
collection | PubMed |
description | Lipid Droplets (LD) are dynamic organelles that originate in the Endoplasmic Reticulum and mostly bud off toward the cytoplasm, where they store neutral lipids for energy and protection purposes. LD also have diverse proteins on their surface, many of which are necessary for the their correct homeostasis. However, these organelles also act as reservoirs of proteins that can be made available elsewhere in the cell. In this sense, they act as sinks that titrate key regulators of many cellular processes. Among the specialized factors that reside on cytoplasmic LD are proteins destined for functions in the nucleus, but little is known about them and their impact on nuclear processes. By screening for nuclear proteins in publicly available LD proteomes, we found that they contain a subset of nucleoporins from the Nuclear Pore Complex (NPC). Exploring this, we demonstrate that LD act as a physiological reservoir, for nucleoporins, that impacts the conformation of NPCs and hence their function in nucleo-cytoplasmic transport, chromatin configuration, and genome stability. Furthermore, our in silico modeling predicts a role for LD-released fatty acids in regulating the transit of nucleoporins from LD through the cytoplasm and to nuclear pores. |
format | Online Article Text |
id | pubmed-7926788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79267882021-03-04 Lipid Droplets Are a Physiological Nucleoporin Reservoir Kumanski, Sylvain Viart, Benjamin T. Kossida, Sofia Moriel-Carretero, María Cells Article Lipid Droplets (LD) are dynamic organelles that originate in the Endoplasmic Reticulum and mostly bud off toward the cytoplasm, where they store neutral lipids for energy and protection purposes. LD also have diverse proteins on their surface, many of which are necessary for the their correct homeostasis. However, these organelles also act as reservoirs of proteins that can be made available elsewhere in the cell. In this sense, they act as sinks that titrate key regulators of many cellular processes. Among the specialized factors that reside on cytoplasmic LD are proteins destined for functions in the nucleus, but little is known about them and their impact on nuclear processes. By screening for nuclear proteins in publicly available LD proteomes, we found that they contain a subset of nucleoporins from the Nuclear Pore Complex (NPC). Exploring this, we demonstrate that LD act as a physiological reservoir, for nucleoporins, that impacts the conformation of NPCs and hence their function in nucleo-cytoplasmic transport, chromatin configuration, and genome stability. Furthermore, our in silico modeling predicts a role for LD-released fatty acids in regulating the transit of nucleoporins from LD through the cytoplasm and to nuclear pores. MDPI 2021-02-22 /pmc/articles/PMC7926788/ /pubmed/33671805 http://dx.doi.org/10.3390/cells10020472 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kumanski, Sylvain Viart, Benjamin T. Kossida, Sofia Moriel-Carretero, María Lipid Droplets Are a Physiological Nucleoporin Reservoir |
title | Lipid Droplets Are a Physiological Nucleoporin Reservoir |
title_full | Lipid Droplets Are a Physiological Nucleoporin Reservoir |
title_fullStr | Lipid Droplets Are a Physiological Nucleoporin Reservoir |
title_full_unstemmed | Lipid Droplets Are a Physiological Nucleoporin Reservoir |
title_short | Lipid Droplets Are a Physiological Nucleoporin Reservoir |
title_sort | lipid droplets are a physiological nucleoporin reservoir |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926788/ https://www.ncbi.nlm.nih.gov/pubmed/33671805 http://dx.doi.org/10.3390/cells10020472 |
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